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- W376052272 abstract "This chapter highlights the cytochemical localization of enzymes in plant cells. Most methods used in the cytochemical detection of plant enzymes are similar to, or derived from, those that have been developed for animal cytochemistry. These include the use of diaminobenzidine (DAB), which is used to detect the activity of oxidases, ferricyanide, cerium chloride, and immunogold labeling. Immunogold techniques are the most powerful and specific methods available for the cytochemical detection of enzymes. The cytochemical stains permit the intracellular localization of some enzymes; however, their use is limited by the nature of the reaction they participate in and by their relative nonspecificity for individual enzymes. Immunological methods demand certain specific requirements for the effective interpretation of data. These include the monospecificity of the antibody used in the localization studies, the techniques used in the fixation and embedding of a plant material, and the condition of incubation used for a specific labeling. Standard biochemical methodology—using a combination of salt fractionation, gel filtration, ion-exchange chromatography, and chromatofocusing—usually permits the isolation of abundant enzymes in a relatively pure state." @default.
- W376052272 created "2016-06-24" @default.
- W376052272 creator A5043664275 @default.
- W376052272 creator A5077016585 @default.
- W376052272 date "1991-01-01" @default.
- W376052272 modified "2023-09-26" @default.
- W376052272 title "Cytochemical Localization of Enzymes in Plant Cells" @default.
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