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- W379735639 abstract "This chapter discusses a study analyzing the immunosensing with commercially available low-loss surface acoustic wave devices. Surface transverse waves (STW) devices were used as the frequency determining element of an oscillator circuit. The adsorption of proteins from solution could be monitored directly by the resulting change in resonant frequency. When uncoated sensors were subjected to spontaneous adsorption of bovine serum albumin (BSA) in buffer solution, the relative frequency changes showed a linear relationship with respect to the operating frequency. This implies that the mass loading effect is dominant in this system. Immunosensors were prepared by coating the STW devices with antibodies. Pretreatment was found to be necessary to improve the stability of the transducer electrodes in aqueous media, while facilitating the covalent immobilization of antibodies. Sensors coated with anti-glucose oxidase showed an irreversible frequency shift when treated with a glucose oxidase solution. This shift was not observed when sensors coated with a different antibody were treated with the same solution. It is concluded that the irreversible shift arises from specific binding of the antigen." @default.
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- W379735639 date "1994-01-01" @default.
- W379735639 modified "2023-10-17" @default.
- W379735639 title "Immunosensing with Commercially Available Low-loss Surface Acoustic Wave Devices" @default.
- W379735639 doi "https://doi.org/10.1016/b978-1-85617-242-4.50247-0" @default.
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