Matches in SemOpenAlex for { <https://semopenalex.org/work/W38036571> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W38036571 endingPage "6" @default.
- W38036571 startingPage "571" @default.
- W38036571 abstract "Conjugation of the protein-reactive drugs D-penicillamine (PA) and benzylpenicillin (BP) to immune cells to generate drug-derived antigenic determinants has been implicated in drug-induced allergies and autoimmunity. We have therefore developed an in vitro system to demonstrate and characterize the formation of cellular antigens by these drugs. Binding of PA and BP to rat peritoneal exudate cells was detected by a cell ELISA, employing rabbit antisera specific for each drug, and an indicator system employing a second antibody coupled to biotin-streptavidin-beta-galactosidase. For both drugs, binding was detected over the concentration range 125-1000 micrograms/ml. PA bound cells rapidly (maximum binding within 10 min), whereas BP bound relatively slowly (maximum binding occurring later than 4 hr). A possible role for intracellular processing and cellular metabolic activity in the generation of these drug-derived antigenic determinants was examined. Pretreatment of the cells with the fixative paraformaldehyde significantly enhanced binding of PA but not BP. Treatment of cells with the lysosomotropic agents ammonium chloride or chloroquine, or with the metabolic inactivator sodium azide, did not affect the binding of either drug compared with untreated control cells. However, treatment with the oxidising agent copper sulphate, or the cellular activator phorbol myristate acetate, did significantly enhance binding of both drugs to the cells. Therefore, binding of PA and BP to the cell surface appears not to require an intracellular processing event to generate a recognizable antigenic determinant, but is enhanced by treatments that stimulate oxidative processes." @default.
- W38036571 created "2016-06-24" @default.
- W38036571 creator A5038606401 @default.
- W38036571 creator A5065400515 @default.
- W38036571 creator A5073774141 @default.
- W38036571 date "1991-04-01" @default.
- W38036571 modified "2023-10-18" @default.
- W38036571 title "Penicillamine and penicillin can generate antigenic determinants on rat peritoneal cells in vitro." @default.
- W38036571 cites W1493082932 @default.
- W38036571 cites W1542088265 @default.
- W38036571 cites W1560987808 @default.
- W38036571 cites W1591443863 @default.
- W38036571 cites W1597843977 @default.
- W38036571 cites W1992577200 @default.
- W38036571 cites W1996374953 @default.
- W38036571 cites W2031069334 @default.
- W38036571 cites W2035876187 @default.
- W38036571 cites W2039387657 @default.
- W38036571 cites W2062595052 @default.
- W38036571 cites W2063001144 @default.
- W38036571 cites W2073203158 @default.
- W38036571 cites W2090958426 @default.
- W38036571 cites W2091317160 @default.
- W38036571 cites W2104901056 @default.
- W38036571 cites W2141306681 @default.
- W38036571 cites W2152280976 @default.
- W38036571 cites W2162581073 @default.
- W38036571 cites W2255788937 @default.
- W38036571 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1384379" @default.
- W38036571 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1709917" @default.
- W38036571 hasPublicationYear "1991" @default.
- W38036571 type Work @default.
- W38036571 sameAs 38036571 @default.
- W38036571 citedByCount "6" @default.
- W38036571 countsByYear W380365712015 @default.
- W38036571 crossrefType "journal-article" @default.
- W38036571 hasAuthorship W38036571A5038606401 @default.
- W38036571 hasAuthorship W38036571A5065400515 @default.
- W38036571 hasAuthorship W38036571A5073774141 @default.
- W38036571 hasConcept C147483822 @default.
- W38036571 hasConcept C153911025 @default.
- W38036571 hasConcept C178790620 @default.
- W38036571 hasConcept C185592680 @default.
- W38036571 hasConcept C202751555 @default.
- W38036571 hasConcept C203014093 @default.
- W38036571 hasConcept C2779348387 @default.
- W38036571 hasConcept C55493867 @default.
- W38036571 hasConcept C79879829 @default.
- W38036571 hasConcept C86803240 @default.
- W38036571 hasConcept C98274493 @default.
- W38036571 hasConceptScore W38036571C147483822 @default.
- W38036571 hasConceptScore W38036571C153911025 @default.
- W38036571 hasConceptScore W38036571C178790620 @default.
- W38036571 hasConceptScore W38036571C185592680 @default.
- W38036571 hasConceptScore W38036571C202751555 @default.
- W38036571 hasConceptScore W38036571C203014093 @default.
- W38036571 hasConceptScore W38036571C2779348387 @default.
- W38036571 hasConceptScore W38036571C55493867 @default.
- W38036571 hasConceptScore W38036571C79879829 @default.
- W38036571 hasConceptScore W38036571C86803240 @default.
- W38036571 hasConceptScore W38036571C98274493 @default.
- W38036571 hasIssue "4" @default.
- W38036571 hasLocation W380365711 @default.
- W38036571 hasOpenAccess W38036571 @default.
- W38036571 hasPrimaryLocation W380365711 @default.
- W38036571 hasRelatedWork W1975781565 @default.
- W38036571 hasRelatedWork W2003865477 @default.
- W38036571 hasRelatedWork W2033530968 @default.
- W38036571 hasRelatedWork W2047469775 @default.
- W38036571 hasRelatedWork W2187331472 @default.
- W38036571 hasRelatedWork W2415321605 @default.
- W38036571 hasRelatedWork W2499534011 @default.
- W38036571 hasRelatedWork W2899084033 @default.
- W38036571 hasRelatedWork W2952200739 @default.
- W38036571 hasRelatedWork W2952309953 @default.
- W38036571 hasVolume "72" @default.
- W38036571 isParatext "false" @default.
- W38036571 isRetracted "false" @default.
- W38036571 magId "38036571" @default.
- W38036571 workType "article" @default.