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• W38417416 abstract "No AccessJournal of Urology1 Dec 1990Comparison of a Polyclonal and Monoclonal Immunoassay for PSA: Need for an International Antigen Standard Howard C.B. Graves, Nancy Wehner, and Thomas A. Stamey Howard C.B. GravesHoward C.B. Graves More articles by this author , Nancy WehnerNancy Wehner More articles by this author , and Thomas A. StameyThomas A. Stamey More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)39789-6AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Two leading commercial immunoassays for prostate specific antigen (the Yang Laboratories polyclonal radioimmunoassay and the Hybritech two-site monoclonal radioimmunometric assay) were compared using independently purified antigen as the calibrator. The Yang polyclonal assay yielded values from 1.4 to 1.9 times higher than the Hybritech monoclonal assay on the same sample, similar to results of previous investigators. When an independent prostate specific antigen calibrator was substituted for the respective kit calibrators, both assays yielded essentially identical results, thereby confirming that differences in assay values arise from differences in the assigned values of the kit calibrators. Otherwise, both assays had similar responses in the high and low range. Recovery studies of prostate specific antigen in various diluents demonstrated that antigen recovery was lowest in diluents with the highest protein content. We conclude that the kit manufacturers have assigned different values to their antigen calibrators. Sample values between the assays can be compared by use of an appropriate conversion table. Differences in their recommended ranges of normal for prostate specific antigen in male serum (zero to 2.5ng./ml. for the Yang assay and zero to 4.0ng./ml. for the Hybritech assay) reflect two factors: a difference in assigned calibrator values and differences in the selection of the “normal” population of men used to define the normal range of values. We urge adoption of an international standard calibrator for prostate specific antigen as a means to correct these differences. © 1990 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byLINK R, SHARIAT S, NGUYEN C, FARR A, WEINBERG A, MORTON R, RICHARDSON B, BERNARD D and SLAWIN K (2018) VARIATION IN PROSTATE SPECIFIC ANTIGEN RESULTS FROM 2 DIFFERENT ASSAY PLATFORMS: CLINICAL IMPACT ON 2,304 PATIENTS UNDERGOING PROSTATE CANCER SCREENINGJournal of Urology, VOL. 171, NO. 6 Part 1, (2234-2238), Online publication date: 1-Jun-2004.Terris M, Pham T, Issa M and Kabalin J (2018) Routine Transition Zone and Seminal Vesicle Biopsies in All Patients Undergoing Transrectal Ultrasound Guided Prostate Biopsies are Not IndicatedJournal of Urology, VOL. 157, NO. 1, (204-206), Online publication date: 1-Jan-1997.Klee G, Dodge L, Zincke H and Oesterling J (2018) Measurement of Serum Prostate Specific Antigen Using Imx Prostate Specific Antigen AssayJournal of Urology, VOL. 151, NO. 1, (94-98), Online publication date: 1-Jan-1994.Takayama T, Vessella R, Brawer M, Noteboom J and Lange P (2018) The Enhanced Detection of Persistent Disease After Prostatectomy with a New Prostate Specific Antigen ImmunoassayJournal of Urology, VOL. 150, NO. 2 Part 1, (374-378), Online publication date: 1-Aug-1993.Kaplan I, Cox R and Bagshaw M (2018) Prostate Specific Antigen after External Beam Radiotherapy for Prostatic Cancer: FollowupJournal of Urology, VOL. 149, NO. 3, (519-522), Online publication date: 1-Mar-1993.Stamey T, Graves H, Wehner N, Ferrari M and Freiha F (2018) Early Detection of Residual Prostate Cancer After Radical Prostatectomy by an Ultrasensitive Assay for Prostate Specific AntigenJournal of Urology, VOL. 149, NO. 4, (787-792), Online publication date: 1-Apr-1993.Terris M, McNeal J, Freiha F and Stamey T (2018) Efficacy of Transrectal Ultrasound-Guided Seminal Vesicle Biopsies in the Detection of Seminal Vesicle Invasion by Prostate CancerJournal of Urology, VOL. 149, NO. 5 Part 1, (1035-1039), Online publication date: 1-May-1993.Stamey T (2018) Diagnosis of Prostate Cancer: A Personal ViewJournal of Urology, VOL. 147, NO. 3 Part 2, (830-832), Online publication date: 1-Mar-1992.White R, Meyers F, Soares S, Miller D and Soriano T (2018) Urinary Prostate Specific Antigen Levels: Role in Monitoring the Response of Prostate Cancer to TherapyJournal of Urology, VOL. 147, NO. 3 Part 2, (947-951), Online publication date: 1-Mar-1992.Oesterling J (2018) Prostate Specific Antigen: A Critical Assessment of the Most Useful Tumor Marker for Adenocarcinoma of the ProstateJournal of Urology, VOL. 145, NO. 5, (907-923), Online publication date: 1-May-1991. Volume 144Issue 6December 1990Page: 1516-1522 Advertisement Copyright & Permissions© 1990 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Howard C.B. Graves More articles by this author Nancy Wehner More articles by this author Thomas A. Stamey More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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