Matches in SemOpenAlex for { <https://semopenalex.org/work/W38466871> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W38466871 abstract "Oxidative stress may play an important role in impaired endothelial NO synthase (eNOS)-dependent dilation of cerebral arterioles during chronic alcohol consumption. The purpose of this study was to examine the effects of acute (3 hours) and chronic (7 days) alcohol on NO synthesis and superoxide production in cultured human brain vascular cells. Primary human brain microvascular endothelial cells (HBMEC) and human brain vascular smooth muscle cells (HBVSMC) were exposed to alcohol (5 and 50 mM) for 3 hours or 7 days. NO synthesis of HBMEC in response to L-arginine and acetylcholine was measured by a NO microsensor. Superoxide production from HBMEC and HBVSMC was measured using lucigenin-enhanced chemiluminescence. In addition, protein expression of eNOS in HBMEC was measured by Western blot analysis. Acute and chronic exposure of low dose alcohol did not alter NO synthesis and superoxide production. Acute exposure of high dose alcohol increased NO production in response to L-arginine, but not acetylcholine. In contrast, chronic exposure of high dose alcohol significantly reduced NO production in response to L-arginine and acetylcholine. In addition, chronic exposure of high dose alcohol significantly enhanced superoxide production in HBVSMC as well as in HBMEC. Furthermore, both acute and chronic exposure of high dose alcohol increased eNOS protein expression. Our findings suggest that chronic exposure of alcohol may reduce NO bioavailability via enhanced superoxide production in human brain vascular cells." @default.
- W38466871 created "2016-06-24" @default.
- W38466871 creator A5002096365 @default.
- W38466871 creator A5006212012 @default.
- W38466871 creator A5013509380 @default.
- W38466871 creator A5024831760 @default.
- W38466871 creator A5042770255 @default.
- W38466871 creator A5042813540 @default.
- W38466871 date "2008-03-01" @default.
- W38466871 modified "2023-09-23" @default.
- W38466871 title "Effects of Alcohol on Nitric Oxide (NO) Synthesis and Superoxide Production in Human Brain Vascular Cells" @default.
- W38466871 doi "https://doi.org/10.1096/fasebj.22.1_supplement.1151.16" @default.
- W38466871 hasPublicationYear "2008" @default.
- W38466871 type Work @default.
- W38466871 sameAs 38466871 @default.
- W38466871 citedByCount "0" @default.
- W38466871 crossrefType "journal-article" @default.
- W38466871 hasAuthorship W38466871A5002096365 @default.
- W38466871 hasAuthorship W38466871A5006212012 @default.
- W38466871 hasAuthorship W38466871A5013509380 @default.
- W38466871 hasAuthorship W38466871A5024831760 @default.
- W38466871 hasAuthorship W38466871A5042770255 @default.
- W38466871 hasAuthorship W38466871A5042813540 @default.
- W38466871 hasConcept C126322002 @default.
- W38466871 hasConcept C134018914 @default.
- W38466871 hasConcept C181199279 @default.
- W38466871 hasConcept C185592680 @default.
- W38466871 hasConcept C2775910092 @default.
- W38466871 hasConcept C2776151105 @default.
- W38466871 hasConcept C2777622882 @default.
- W38466871 hasConcept C2778326061 @default.
- W38466871 hasConcept C2779127771 @default.
- W38466871 hasConcept C2780795997 @default.
- W38466871 hasConcept C48349386 @default.
- W38466871 hasConcept C519581460 @default.
- W38466871 hasConcept C55493867 @default.
- W38466871 hasConcept C71924100 @default.
- W38466871 hasConcept C98274493 @default.
- W38466871 hasConceptScore W38466871C126322002 @default.
- W38466871 hasConceptScore W38466871C134018914 @default.
- W38466871 hasConceptScore W38466871C181199279 @default.
- W38466871 hasConceptScore W38466871C185592680 @default.
- W38466871 hasConceptScore W38466871C2775910092 @default.
- W38466871 hasConceptScore W38466871C2776151105 @default.
- W38466871 hasConceptScore W38466871C2777622882 @default.
- W38466871 hasConceptScore W38466871C2778326061 @default.
- W38466871 hasConceptScore W38466871C2779127771 @default.
- W38466871 hasConceptScore W38466871C2780795997 @default.
- W38466871 hasConceptScore W38466871C48349386 @default.
- W38466871 hasConceptScore W38466871C519581460 @default.
- W38466871 hasConceptScore W38466871C55493867 @default.
- W38466871 hasConceptScore W38466871C71924100 @default.
- W38466871 hasConceptScore W38466871C98274493 @default.
- W38466871 hasIssue "S1" @default.
- W38466871 hasLocation W384668711 @default.
- W38466871 hasOpenAccess W38466871 @default.
- W38466871 hasPrimaryLocation W384668711 @default.
- W38466871 hasRelatedWork W1997419966 @default.
- W38466871 hasRelatedWork W2038547315 @default.
- W38466871 hasRelatedWork W2054852394 @default.
- W38466871 hasRelatedWork W2077267085 @default.
- W38466871 hasRelatedWork W2088740123 @default.
- W38466871 hasRelatedWork W2091193879 @default.
- W38466871 hasRelatedWork W2114204950 @default.
- W38466871 hasRelatedWork W2146661035 @default.
- W38466871 hasRelatedWork W2148645037 @default.
- W38466871 hasRelatedWork W2415590802 @default.
- W38466871 hasVolume "22" @default.
- W38466871 isParatext "false" @default.
- W38466871 isRetracted "false" @default.
- W38466871 magId "38466871" @default.
- W38466871 workType "article" @default.