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- W38965776 abstract "Conformation-sensitive gel electrophoresis (CSGE) has been introduced as the most reliable method for the screening of large and multi-exon genes because of its simplicity, sensitivity and specificity. Based on heteroduplex formation and with the use of mildly denaturing solvents, it allows detection of single-base mutations with accuracy. This is important in genes such as BRCA1 and BRCA2, in which alterations span the entire gene. We have adapted the CSGE assay to a fluorescent platform--a DNA sequencer one-color technology--that reduces the time involved and enhances resolving power for the complete scanning of the BRCA genes. Electrophoresis has high sensitivity and is performed in less than three hours, and the gel does not require staining with ethidium bromide. Eighteen single-base and six frameshift mutations in the BRCA1 gene were analyzed. We compared the manual and fluorescent CSGE methods, and all mutations were detected with accuracy." @default.
- W38965776 created "2016-06-24" @default.
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- W38965776 date "2000-05-01" @default.
- W38965776 modified "2023-09-25" @default.
- W38965776 title "Adaptation of Conformation-Sensitive Gel Electrophoresis to an ALFexpress<sup>TM</sup> DNA Sequencer to Screen <i>BRCA1</i> Mutations" @default.
- W38965776 cites W2071937923 @default.
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- W38965776 doi "https://doi.org/10.2144/00285rr08" @default.
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