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- W39006978 abstract "Loop-mediated isothermal amplification (LAMP) is a technique involving the use of four to six primers (two inner primers, two outer primers, and two loop primers) and the strand displacement activity of Bacillus subtilis-derived (Bst) DNA polymerase. The end result of strand displacement and loop formation and synthesis is the single-temperature amplification of a highly specific fragment from a DNA template at a much greater titre than that obtained with polymerase chain reaction. With LAMP, there are several methods to determine a positive reaction. Presented here are three alternative methods: gel electrophoresis, hydroxynaphthol blue colorimetric dye, and the fluorescent intercalating PicoGreen(®) reagent." @default.
- W39006978 created "2016-06-24" @default.
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- W39006978 date "2012-01-01" @default.
- W39006978 modified "2023-09-24" @default.
- W39006978 title "Loop-Mediated Isothermal Amplification for the Detection of Plant Pathogens" @default.
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- W39006978 doi "https://doi.org/10.1007/978-1-61779-609-8_13" @default.
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