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- W39012620 abstract "Fecal samples were collected from 170 beef steers during August 2011, and animals that were high-shedders and low-shedders of STEC were identified as described previously (2013 Nebraska Beef Cattle Report, pp. 92-93). DNA was extracted and purified from 48 of the highest shedders and 48 low-shedders using the MagMAXTM Pathogen RNA/DNA Kit (Life Technologies Corp., Carlsbad, Calif.) according to manufacturer’s instructions. The V1-V3 regions of the 16S rRNA genes from the fecal bacterial community of each fecal sample were amplified using the polymerase chain reaction (PCR) technique. The resulting amplicons were multiplexed and were subsequently sequenced at the Genome Center, University of Oklahoma, using 454 pyrosequencing (www.454.com/). The resulting sequence data were analyzed using the published bioinformatic pipelines of MOTHUR (www.mothur.org/) and QIIME (qiime.org/)." @default.
- W39012620 created "2016-06-24" @default.
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- W39012620 date "2014-01-01" @default.
- W39012620 modified "2023-09-27" @default.
- W39012620 title "Differences in Fecal Bacterial Community CompositionBetween Beef Steers which are High-Shedders andLow-Shedders of Shiga Toxin-Producing Escherichia coli (STEC)" @default.
- W39012620 hasPublicationYear "2014" @default.
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