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- W39131451 abstract "The development of resistance to a post-harvest and preplanting benzimidazole fungicide, thiabendazole (TBZ), has resulted in the increase of silver scurf of potato caused by Helminthosporium solani . Conventional and polymerase chain reaction (PCR)-based assays were compared, among 54 isolates of H. solani , to detect the TBZ-sensitive (TBZ S ) and -resistant (TBZ R ) isolates. In a PCR-based assay, all 27 single-spore isolates from eastern Canada, nine isolates from Alberta in western Canada, six isolates from North Dakota, USA were distinguished as TBZ R , the remainder of the isolates being determined as TBZ S . The results from the PCR-based assay correlated well with the TBZ sensitivity assessment of isolates of H. solani on the TBZ-amended medium. Also, the detection of TBZ R - H. solani in 20 tuber samples with silver scurf lesions by the PCR-based assay demonstrates that this method can be used to detect the pathogen directly from the tuber lesions. The PCR-based method was more rapid (1 day) than the conventional technique (5 weeks)." @default.
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- W39131451 date "2005-04-12" @default.
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- W39131451 title "Comparison of methods to detect resistance of Helminthosporium solani to a thiabendazole fungicide" @default.
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- W39131451 doi "https://doi.org/10.7202/706220ar" @default.
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