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- W39222777 abstract "We describe a protocol to screen for protein-protein interactions using the Gal-4 based yeast two-hybrid system. In this protocol, we describe serial transformation of bait into an already constructed cDNA library in yeast AH109 cells. We find this method to gives the most number of true interactions. Where a pre-made library in yeast cells is not available, the method outlined can be quickly adapted. AH109 cells can be first transformed with bait containing a vector followed by selection of yeast containing the bait. A second transformation of yeast cells is then accomplished with the cDNA library. The method is quick and can lead to the discovery of significant interactions." @default.
- W39222777 created "2016-06-24" @default.
- W39222777 creator A5074978959 @default.
- W39222777 date "2016-01-01" @default.
- W39222777 modified "2023-09-27" @default.
- W39222777 title "Yeast Two-Hybrid Screening to Test for Protein–Protein Interactions in the Auditory System" @default.
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- W39222777 doi "https://doi.org/10.1007/978-1-4939-3615-1_6" @default.
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