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• W39614084 abstract "This chapter describes techniques commonly used to study cell death using in vitro cell culture systems. These techniques include a range of simple dye exclusion assays that give crude information on cell viability but do not necessarily discriminate between apoptotic or necrotic modes of cell death. The chapter describes cell death assays that are based on the observation that apoptosis is accompanied by DNA fragmentation, either into the classical “ladder” pattern of 200 bp integer multiples, 50kb fragments, or single-stranded DNA cleavage. Assays used to measure this fragmentation include gel electrophoresis of total DNA, quantification of the release of radioactively labeled DNA, examination of cell cycle profile, and in situ nick translation. Because apoptotic cells are morphologically highly distinctive and are easily distinguishable from both viable and necrotic cells, most cell death assays should be coupled with direct morphological evaluation of the cell population under study to define with certainty the mode of cell death that is occurring." @default.
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• W39614084 date "1995-01-01" @default.
• W39614084 modified "2023-10-18" @default.
• W39614084 title "Chapter 9 The End of the (Cell) Line: Methods for the Study of Apoptosis in Vitro" @default.
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• W39614084 doi "https://doi.org/10.1016/s0091-679x(08)61929-9" @default.
• W39614084 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7541883" @default.
• W39614084 hasPublicationYear "1995" @default.
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