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- W39657844 abstract "Genes encoding at least five polypeptides are required to produce the enzymes necessary for bioluminescence in marine bacteria. These include genes coding for the two subunits of luciferase (α and β) and the three polypeptides of a fatty acid reductase complex, which supplies aldehyde for the bioluminescent reaction. Two regulatory polypeptides have been identified in the Vibriofischeri luminescence system. Transfer of the genes from luminescent bacteria into Escherichia coli and the study of their regulation can now readily be accomplished as very rapid and highly sensitive techniques are available for detection of the cloned genes and the analyses of the mRNAs and gene products. Selection of clones containing genes from the bioluminescence operons is generally based on the detection of light emission. Only the luciferase genes need be present as the aldehyde substrate can be supplied exogenously to E. coli and sufficient FMNH2 and O2 are present intracellularly to allow generation of light. Screening may be accomplished by the following approaches: visual scanning, exposure of colonies to film, scintillation counter readings, and hybridization probes." @default.
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- W39657844 date "1986-01-01" @default.
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- W39657844 title "[7] Cloning and expression of the genes from the bioluminescent system of marine bacteria" @default.
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- W39657844 doi "https://doi.org/10.1016/0076-6879(86)33056-8" @default.
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