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- W398653608 abstract "Introduction: ALS is a rapidly progressive neurodegenerative disease. Diagnosis and prognosis estimation are often delayed because there are no reliable biomarkers. In this scenario, microRNAs may be good candidates because they are released into the circulation by pathologically affected tissues and display remarkable stability in body fluids.Objectives: to identify microRNAs differentially expressed in the skeletal muscle and plasma of ALS patients when compared to healthy controls.Method: We perfomed biopsies of Biceps brachii muscles from five patients with ALS (El Escorial criteria) and five healthy controls. MicroRNAs enriched fraction was isolated with miRNeasy Kit (Qiagen). The expression profile of microRNAs was evaluated using the GeneChip miRNA Arrays platform (Affymetrix). For background correction, normalization and summarization of data, we employed the RMA (Robust Multi-array Average) algorithm. Differential expression of microRNAs between patients and controls was evaluated through RankProd with correction for multiple comparisons (FDR p <0.05). The results were validated by qPCR. We then assessed the plasmatic expression of these candidate microRNAs through qPCR in a set of 40 patients and 40 controls using Student t-test.Results: Microarray analyses identified 11 differentially expressed microRNAs in the muscle of ALS patients: hsa-miR-424, hsa-miR-503, hsa-miR-886-3p, hsa-miR-542-5p, hsa-miR-34a, hsa-miR-886-5p, hsa-miR-146b-5p, hsa-miR-504, hsa-miR-21, hsa-miR-214 and hsa-miR-183. Hsa-miR424, hsa-miR-214 and has-miR-206 were validated by qPCR in muscle samples. In plasma, we found that only hsa-miR-424 and has-miR 206 were significantly overexpressed in ALS patients (RQ1.37 ± 1.65 vs 0.51± 0.91, p=0.006 and 4.61± 5.36 vs 0.90 ± 0.24 p<0.001, respectively).Conclusion: Hsa-miR-424 and has-miR-206 are overexpressed in the skeletal muscle and plasma of ALS patients. These are promising candidate biomarkers that need to be validated in larger samples with available clinical data. Disclosure: Dr. Andrade has nothing to disclose. Dr. Albuquerque has nothing to disclose. Dr. Peluzzo has nothing to disclose. Dr. Dogni has nothing to disclose. Dr. Nucci has nothing to disclose. Dr. Lopes-Cendes has nothing to disclose. Dr. Franca, Jr. has nothing to disclose. Dr. Avansini has nothing to disclose." @default.
- W398653608 created "2016-06-24" @default.
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- W398653608 date "2015-04-06" @default.
- W398653608 modified "2023-10-17" @default.
- W398653608 title "Hsa-miR-424 and has-miR-206 are overexpressed in the skeletal muscle and plasma of patients with Amyotrophic Lateral Sclerosis (ALS) (P2.054)" @default.
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