Matches in SemOpenAlex for { <https://semopenalex.org/work/W398996895> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W398996895 abstract "Non tuberculous mycobacteria (99) comprising 34 isolates from animal specimens, 57 soil and 8 water samples from animal dwellings were identified on the basis of growth rate, pigmentation, colony morphology and biochemical reactions. Of the 8 species recovered from animal specimens 4 (M. fortuitum, M. smegmatis , M. chelonae and M. abscessus) were prevalent in soil while only 2 of these species (M. fortuitum and M. smegmatis) prevailed in water samples. These isolates along with 13 reference strains of mycobacteria were subjected to polymerase chain reaction-restriction fragment length polymorphism analysis (PRA) of hsp65 and rpoB genes. All the isolates generated products of 439 bp and 360 bp in PCR of hsp65 and rpoB genes respectively. Restriction digestion of the product of hsp65 and BstEll and Haelll and that of rpoB with Mspl and Haelll in separate reactions and analysis of digests by agarose gel electrophoresis revealed RFLP patterns characteristic for most of the species tested. Out of the 16-mycobacterial species evaluated, 13 produced a single PRA pattern, while 3 species generated 2 patterns in both the PRA methods. M. abcessus and M. chelonae were distinguishable from each other. M. fortuitum isolates were differentiated into M. fortuitum I and M. fortuitum II, M. avium complex isolates into M. intracellulare and M. avium, M. kansasii isolates into M. kansasii I and M. kansasii V and M. gordonae into M. gordonae II and M. gordonae (new type) by both the methods. Both the PRA methods could not make a distinction between M. tuberculosis and M. bovis however, PRA with hsp65 was found better since it yielded relatively large sized and distinct band." @default.
- W398996895 created "2016-06-24" @default.
- W398996895 creator A5065098385 @default.
- W398996895 creator A5067100026 @default.
- W398996895 date "2007-01-01" @default.
- W398996895 modified "2023-09-26" @default.
- W398996895 title "PCR- RFLP analysis (PRA) of non tuberculous mycobacteria (NTM) isolated from animal specimens and environment using hsp65 and rpoB genes" @default.
- W398996895 hasPublicationYear "2007" @default.
- W398996895 type Work @default.
- W398996895 sameAs 398996895 @default.
- W398996895 citedByCount "0" @default.
- W398996895 crossrefType "journal-article" @default.
- W398996895 hasAuthorship W398996895A5065098385 @default.
- W398996895 hasAuthorship W398996895A5067100026 @default.
- W398996895 hasConcept C104317684 @default.
- W398996895 hasConcept C168007829 @default.
- W398996895 hasConcept C2777931070 @default.
- W398996895 hasConcept C2778271344 @default.
- W398996895 hasConcept C2778340795 @default.
- W398996895 hasConcept C2778679867 @default.
- W398996895 hasConcept C2780374374 @default.
- W398996895 hasConcept C2780931024 @default.
- W398996895 hasConcept C42062724 @default.
- W398996895 hasConcept C49105822 @default.
- W398996895 hasConcept C523546767 @default.
- W398996895 hasConcept C54355233 @default.
- W398996895 hasConcept C86803240 @default.
- W398996895 hasConcept C89423630 @default.
- W398996895 hasConceptScore W398996895C104317684 @default.
- W398996895 hasConceptScore W398996895C168007829 @default.
- W398996895 hasConceptScore W398996895C2777931070 @default.
- W398996895 hasConceptScore W398996895C2778271344 @default.
- W398996895 hasConceptScore W398996895C2778340795 @default.
- W398996895 hasConceptScore W398996895C2778679867 @default.
- W398996895 hasConceptScore W398996895C2780374374 @default.
- W398996895 hasConceptScore W398996895C2780931024 @default.
- W398996895 hasConceptScore W398996895C42062724 @default.
- W398996895 hasConceptScore W398996895C49105822 @default.
- W398996895 hasConceptScore W398996895C523546767 @default.
- W398996895 hasConceptScore W398996895C54355233 @default.
- W398996895 hasConceptScore W398996895C86803240 @default.
- W398996895 hasConceptScore W398996895C89423630 @default.
- W398996895 hasIssue "12" @default.
- W398996895 hasLocation W3989968951 @default.
- W398996895 hasOpenAccess W398996895 @default.
- W398996895 hasPrimaryLocation W3989968951 @default.
- W398996895 hasRelatedWork W1821201661 @default.
- W398996895 hasRelatedWork W1975798583 @default.
- W398996895 hasRelatedWork W1981237054 @default.
- W398996895 hasRelatedWork W1999208250 @default.
- W398996895 hasRelatedWork W2066044785 @default.
- W398996895 hasRelatedWork W2142864236 @default.
- W398996895 hasRelatedWork W2143566571 @default.
- W398996895 hasRelatedWork W2147182276 @default.
- W398996895 hasRelatedWork W2335383425 @default.
- W398996895 hasRelatedWork W2380674500 @default.
- W398996895 hasRelatedWork W2405112964 @default.
- W398996895 hasRelatedWork W2406825439 @default.
- W398996895 hasRelatedWork W2415706278 @default.
- W398996895 hasRelatedWork W2530286322 @default.
- W398996895 hasRelatedWork W2744414730 @default.
- W398996895 hasRelatedWork W2892406450 @default.
- W398996895 hasRelatedWork W2901487097 @default.
- W398996895 hasRelatedWork W2996217833 @default.
- W398996895 hasRelatedWork W2045122316 @default.
- W398996895 hasRelatedWork W2182989246 @default.
- W398996895 hasVolume "77" @default.
- W398996895 isParatext "false" @default.
- W398996895 isRetracted "false" @default.
- W398996895 magId "398996895" @default.
- W398996895 workType "article" @default.