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- W401403912 abstract "At seedling stage of papaya,plant sexes of papaya were identified by cDNA-AFLP technique and the difference of genes expression of plant sex,and the sequences and functions of differential expression genes were analyzed to discuss the molecular mechanism on regulating the sex differentiation of papaya.The results showed that the total obtained stable bands of PCR amplification were 25-75,and there was obvious difference in sex gene expressions of three kinds of papaya seedlings.Among them,the bands of male plant were 14,these of female plant were 13 and those of hermaphrodite were 9.The sequences of partial difference TDFs were compared to all sequences of GenBank by Blastx and Blastn,and the sequences of some differential fragments were unknown functional genes,and the sequences of some frag-ments were highly homologous to the genes of glutathione transferase,related genes of plant steroid hormone synthesis,genes of mitogen-activated protein kinases,nucleic acid sequences tied to telomerase,unknown functional genes cloned from the leaves of populus and flower and bud of arabidopsis.Therefore,cDNA-AFLP technique could be used for identi-fying the plant sex of papaya,and the differential expression of sex genes may be the integrated reaction of regulating the plant sex development and growth development by a series of functional genes in papaya." @default.
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- W401403912 date "2009-01-01" @default.
- W401403912 modified "2023-09-27" @default.
- W401403912 title "cDNA-AFLP analysis of genes expression for different sexes of Carica papaya L." @default.
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