Matches in SemOpenAlex for { <https://semopenalex.org/work/W405780046> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W405780046 abstract "Kimutattuk, hogy a glutation-transzport sebessege jol korrelal a rianodin receptor kalcium csatorna (RyR) egyik izoformaja, a RyR1 mennyisegevel. Kulonboző RyR gatloszerek hatekonyan gatoltak, RyR agonistak pedig aktivaltak a transzportot. Osszehasonlitottuk a glutation transzport sajatossagait RyR1 csatornat expresszalo transzfektalt, illetve vad tipusu HEK293 sejtekből izolalt mikroszomakon. Eredmenyeink azt bizonyitjak, hogy a RyR1 kozvetlenul vesz reszt a glutation transzportjaban. Megallapitottuk tovabba, hogy a RyR1 csatorna magas intraluminalis glutation szint eseten rezisztensse valik egyes gatloszereivel szemben. Kimutattuk, hogy az ER lumenben elhelyezkedő hexoz-6-foszfat dehidrogenaz fenntartja a lokalis redukalt NADPH szintet. Igazoltuk, hogy a glutation reduktaz enzim, amely a sejt egyeb reszein a tiol/diszulfid es a piridin nukleotid redox rendszerek kozotti kapcsolatot biztositja, nincs jelen az ER lumenben. Skorbutizalt tengerimalacok majaban a skorbut kialkulasaval parhuzamosan az ER stressz jeleit es fokozott apoptozist eszleltuk. A kimutatott ER-stressz es apoptozis alatamasztja az aszkorbat szerepet a feherje-foldingban. Kidolgoztunk egy uj modszert, amely lehetőve teszi jeloletlen molekulak mikroszomalis transzportjanak tanulmanyozasat. Az uj modszer tobb vegyulet egyidejű transzportjanak meresere es a ligand stabilitasanak nyomonkovetesere is alkalmas, erzekenysege hasonlo a radioaktiv detektalasehoz, viszont gazdasagosabb annal. | We found a correlation between the rate of glutathione transport and the abundance of an isoform of ryanodine receptor calcium channel (RyR1). The transport was inhibited or activated by various RyR antagonists or agonists, respectively. The characteristics of glutathione transport were compared in microsomes prepared from RyR1 expressing transfected and wild type HEK293 cells. Our results prove the direct involvement of RyR1 in glutathione transport. In addition, we found that RyR1 channel becomes resistant to some of its inhibitors at high luminal glutathione levels. We observed that hexose 6-phosphate dehydrogenase maintains the reduced NADPH pool in the ER lumen. We proved that glutathione reductase - that couples the thiol/disulfide and pyridine nucleotide redox systems in other cellular compartments - is not present in the ER. We detected the indicators of ER stress and enhanced apoptosis in the liver of scorbutic guinea pigs. These findings further support the hypothesis that ascorbate participates in the process of protein folding in the ER. We developed a new method that allows the investigation of the microsomal transport of unlabeled compounds. The novel technique is suitable for the detection of simultaneous multiple transport processes and for the monitoring of the ligand stability. Its sensitivity is similar to that of the transport measurements based on radiodetection." @default.
- W405780046 created "2016-06-24" @default.
- W405780046 creator A5019904199 @default.
- W405780046 creator A5045878220 @default.
- W405780046 creator A5068853112 @default.
- W405780046 creator A5075960840 @default.
- W405780046 date "2006-01-01" @default.
- W405780046 modified "2023-09-27" @default.
- W405780046 title "Antioxidáns anyagcsere és transzportfolyamatok az endo/szarkoplazmás retikulumban. = Antioxidant metabolism and transport processes in the endo/sarcoplasmic reticulum." @default.
- W405780046 hasPublicationYear "2006" @default.
- W405780046 type Work @default.
- W405780046 sameAs 405780046 @default.
- W405780046 citedByCount "0" @default.
- W405780046 crossrefType "journal-article" @default.
- W405780046 hasAuthorship W405780046A5019904199 @default.
- W405780046 hasAuthorship W405780046A5045878220 @default.
- W405780046 hasAuthorship W405780046A5068853112 @default.
- W405780046 hasAuthorship W405780046A5075960840 @default.
- W405780046 hasConcept C104317684 @default.
- W405780046 hasConcept C113217602 @default.
- W405780046 hasConcept C153911025 @default.
- W405780046 hasConcept C158617107 @default.
- W405780046 hasConcept C181199279 @default.
- W405780046 hasConcept C185592680 @default.
- W405780046 hasConcept C2776333580 @default.
- W405780046 hasConcept C53345823 @default.
- W405780046 hasConcept C538909803 @default.
- W405780046 hasConcept C55493867 @default.
- W405780046 hasConcept C86803240 @default.
- W405780046 hasConcept C87644729 @default.
- W405780046 hasConcept C95444343 @default.
- W405780046 hasConceptScore W405780046C104317684 @default.
- W405780046 hasConceptScore W405780046C113217602 @default.
- W405780046 hasConceptScore W405780046C153911025 @default.
- W405780046 hasConceptScore W405780046C158617107 @default.
- W405780046 hasConceptScore W405780046C181199279 @default.
- W405780046 hasConceptScore W405780046C185592680 @default.
- W405780046 hasConceptScore W405780046C2776333580 @default.
- W405780046 hasConceptScore W405780046C53345823 @default.
- W405780046 hasConceptScore W405780046C538909803 @default.
- W405780046 hasConceptScore W405780046C55493867 @default.
- W405780046 hasConceptScore W405780046C86803240 @default.
- W405780046 hasConceptScore W405780046C87644729 @default.
- W405780046 hasConceptScore W405780046C95444343 @default.
- W405780046 hasLocation W4057800461 @default.
- W405780046 hasOpenAccess W405780046 @default.
- W405780046 hasPrimaryLocation W4057800461 @default.
- W405780046 hasRelatedWork W1009292734 @default.
- W405780046 hasRelatedWork W1016295290 @default.
- W405780046 hasRelatedWork W1028273558 @default.
- W405780046 hasRelatedWork W122345408 @default.
- W405780046 hasRelatedWork W123074350 @default.
- W405780046 hasRelatedWork W2592076638 @default.
- W405780046 hasRelatedWork W38555975 @default.
- W405780046 hasRelatedWork W436634874 @default.
- W405780046 hasRelatedWork W51911584 @default.
- W405780046 hasRelatedWork W606540316 @default.
- W405780046 hasRelatedWork W62716992 @default.
- W405780046 hasRelatedWork W90436090 @default.
- W405780046 hasRelatedWork W955811329 @default.
- W405780046 hasRelatedWork W981496541 @default.
- W405780046 hasRelatedWork W986411262 @default.
- W405780046 hasRelatedWork W986809079 @default.
- W405780046 hasRelatedWork W992978482 @default.
- W405780046 hasRelatedWork W108987963 @default.
- W405780046 hasRelatedWork W170608386 @default.
- W405780046 hasRelatedWork W994214728 @default.
- W405780046 isParatext "false" @default.
- W405780046 isRetracted "false" @default.
- W405780046 magId "405780046" @default.
- W405780046 workType "article" @default.