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- W40710801 abstract "A number of mechanical cell stimulators have been used to study the effect of mechanical stimulation on cells in vitro. But theefficiency of these devices is not fully desirable. We recently developed a new device for mechanical cell stimulation, the centrifugal forcestretcher, and compared its efficacy with that of the traditional Flexercell Strain Unit. When the mechanical stretcher circumrotates withcertain speed, cardiac myocytes attached on the plate are stretched and elongated by centrifugal force. Neonatal rat cardiac myocytes wereisolated by enzymatic dissociation from the hearts of 3～5 d old Sprague Dawley rats, and were mechanically stimulated by traditional 20%stretch and 180 r/min centrifugal force for 12 and 24 h. The effects of mechanical stimulation on the hypertrophic response of neonatal ratcardiac myocytes and production of angiotensin Ⅱ (Ang Ⅱ) were examined. Compared with the non-stretch group, the radioactivity of~3H-leucine incorporated into the streteh-stimulated cardiac myocytes in the centrifugal force stretch group was significanty higher [(1295.17±51.19) vs (1122.67±51.63) in 12 h; (1447.5±35.96) vs (1210.67±90.92) in 24 h, P0.05]. Ang Ⅱ was also dramaticallyincreased by 128% in 12 h (P0.05) and 139% in 24 h (P0.01). After the myocytes was stretched for 24 h, the LDH level in the mediumin the Flexercell Strain Unit group was significanly higher than that in the centrifugal force group [(14.5±8.7) U/L vs (7.8±4.3) U/L,P0.05]. The centrifugal force stretcher is a new and improved mechanical cell stimulator with the same effects on the protein synthesisand Ang Ⅱ secretion of the cardiac myocytes, and the damage to the cells bronght by this stimulator is relatively slighter in comparisonwith the Flexercell Strain Unit." @default.
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- W40710801 date "2004-06-25" @default.
- W40710801 modified "2023-09-23" @default.
- W40710801 title "Centrifugal force stretcher a new of in vitro mechanical cell stimulator." @default.
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