Matches in SemOpenAlex for { <https://semopenalex.org/work/W408443602> ?p ?o ?g. }
- W408443602 endingPage "101" @default.
- W408443602 startingPage "95" @default.
- W408443602 abstract "Human PON1 (h-PON1) is a Ca(2+)-dependent serum enzyme and can hydrolyze (and inactivate) a wide range of substrates. It is a multifaceted enzyme and exhibit anti-inflammatory, anti-oxidative, anti-atherogenic, anti-diabetic, anti-microbial, and organophosphate (OP)-detoxifying properties. Thus, h-PON1 is a strong candidate for the development of therapeutic intervention against these conditions in humans. Insufficient hydrolyzing activity of native h-PON1 against desirable substrate affirms the urgent need to develop improved variant(s) of h-PON1 having enhanced activity. Production of recombinant h-PON1 (rh-PON1) using an Escherichia coli expression system is a key to develop such variant(s). However, generation of rh-PON1 using E. coli expression system has been elusive until now because of the aggregation of over-expressed rh-PON1 protein in inactive form as inclusion bodies (IBs) in the bacterial cells. In this study, we have over-expressed rh-PON1(wt) and rh-PON1(H115W;R192K) proteins as IBs in E. coli, and refolded the inactive enzymes present in the IBs to their active form using in vitro refolding. The active enzymes were isolated from the refolding mixture by ion-exchange chromatography. The catalytic properties of the refolded enzymes were similar to their soluble counterparts. Our results show that the pure and the active variant of rh-PON1 enzyme having enhanced hydrolyzing activity can be produced in large quantities using E. coli expression system. This method can be used for the industrial scale production of rh-PON1 enzymes and will aid in developing h-PON1 as a therapeutic candidate." @default.
- W408443602 created "2016-06-24" @default.
- W408443602 creator A5001312801 @default.
- W408443602 creator A5020729159 @default.
- W408443602 creator A5055646685 @default.
- W408443602 creator A5079864505 @default.
- W408443602 date "2015-11-01" @default.
- W408443602 modified "2023-09-26" @default.
- W408443602 title "Expression and purification of biologically active recombinant human paraoxonase 1 from inclusion bodies of Escherichia coli" @default.
- W408443602 cites W1503328074 @default.
- W408443602 cites W1707439038 @default.
- W408443602 cites W1918304938 @default.
- W408443602 cites W1966197898 @default.
- W408443602 cites W1970756887 @default.
- W408443602 cites W1973033448 @default.
- W408443602 cites W1980765563 @default.
- W408443602 cites W1988874714 @default.
- W408443602 cites W1992760973 @default.
- W408443602 cites W1997181066 @default.
- W408443602 cites W2010783985 @default.
- W408443602 cites W2021353230 @default.
- W408443602 cites W2022865333 @default.
- W408443602 cites W2023508534 @default.
- W408443602 cites W2026999474 @default.
- W408443602 cites W2028461731 @default.
- W408443602 cites W2029195391 @default.
- W408443602 cites W2031923603 @default.
- W408443602 cites W2040103562 @default.
- W408443602 cites W2043748991 @default.
- W408443602 cites W2050082784 @default.
- W408443602 cites W2065872061 @default.
- W408443602 cites W2070301612 @default.
- W408443602 cites W2071001421 @default.
- W408443602 cites W2072402720 @default.
- W408443602 cites W2073899730 @default.
- W408443602 cites W2081927835 @default.
- W408443602 cites W2082539135 @default.
- W408443602 cites W2086101157 @default.
- W408443602 cites W2090957588 @default.
- W408443602 cites W2091972016 @default.
- W408443602 cites W2111760376 @default.
- W408443602 cites W2114682122 @default.
- W408443602 cites W2144220988 @default.
- W408443602 cites W2145154276 @default.
- W408443602 cites W2146768540 @default.
- W408443602 cites W2150821913 @default.
- W408443602 cites W2153753900 @default.
- W408443602 cites W2155843143 @default.
- W408443602 cites W2156092482 @default.
- W408443602 cites W2159196969 @default.
- W408443602 cites W2161414810 @default.
- W408443602 cites W2161964918 @default.
- W408443602 cites W2163222586 @default.
- W408443602 cites W2164259487 @default.
- W408443602 cites W2164967692 @default.
- W408443602 cites W2210894881 @default.
- W408443602 cites W89653608 @default.
- W408443602 cites W2043904595 @default.
- W408443602 doi "https://doi.org/10.1016/j.pep.2015.05.011" @default.
- W408443602 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26003526" @default.
- W408443602 hasPublicationYear "2015" @default.
- W408443602 type Work @default.
- W408443602 sameAs 408443602 @default.
- W408443602 citedByCount "14" @default.
- W408443602 countsByYear W4084436022016 @default.
- W408443602 countsByYear W4084436022017 @default.
- W408443602 countsByYear W4084436022018 @default.
- W408443602 countsByYear W4084436022019 @default.
- W408443602 countsByYear W4084436022020 @default.
- W408443602 countsByYear W4084436022022 @default.
- W408443602 countsByYear W4084436022023 @default.
- W408443602 crossrefType "journal-article" @default.
- W408443602 hasAuthorship W408443602A5001312801 @default.
- W408443602 hasAuthorship W408443602A5020729159 @default.
- W408443602 hasAuthorship W408443602A5055646685 @default.
- W408443602 hasAuthorship W408443602A5079864505 @default.
- W408443602 hasConcept C104317684 @default.
- W408443602 hasConcept C135763542 @default.
- W408443602 hasConcept C153911025 @default.
- W408443602 hasConcept C181199279 @default.
- W408443602 hasConcept C185592680 @default.
- W408443602 hasConcept C203750385 @default.
- W408443602 hasConcept C2776509667 @default.
- W408443602 hasConcept C28212737 @default.
- W408443602 hasConcept C37501387 @default.
- W408443602 hasConcept C40767141 @default.
- W408443602 hasConcept C547475151 @default.
- W408443602 hasConcept C55493867 @default.
- W408443602 hasConcept C86803240 @default.
- W408443602 hasConceptScore W408443602C104317684 @default.
- W408443602 hasConceptScore W408443602C135763542 @default.
- W408443602 hasConceptScore W408443602C153911025 @default.
- W408443602 hasConceptScore W408443602C181199279 @default.
- W408443602 hasConceptScore W408443602C185592680 @default.
- W408443602 hasConceptScore W408443602C203750385 @default.
- W408443602 hasConceptScore W408443602C2776509667 @default.
- W408443602 hasConceptScore W408443602C28212737 @default.
- W408443602 hasConceptScore W408443602C37501387 @default.