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• W409687597 abstract "Eighteen phenotypically and biochemically distinct Gymnema accessions representing different geographical regions of Kerala were characterized using isozymes and RAPD markers. In the isozyme analysis, three enzyme systems viz., malate dehydrogenase, esterase, and RUBISCO were studied. Five out of the eight resolved loci (62.5%) were polymorphic with the number of alleles expressed at the polymorphic loci being 10. RUBISCO activity was monomorphic across the accessions. In the RAPD assay, 123 amplified products were generated using 15 selected random primers. Of these, 90 bands were polymorphic with the total frequency of polymorphic markers as high as 73.2%. Specific bands were obtained for five accessions. Jaccard’s coefficient ranged between 0.72 and 0.85 indicating a moderate level of variability. Cluster analysis of RAPD data using Unweighted Pair Group Method of Arithmetic Averages produced two major clusters and three sub-clusters. Overall, molecular fingerprinting revealed the existence of considerable genetic variations in the Gymnema germplasm collection from Kerala." @default.
• W409687597 created "2016-06-24" @default.
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• W409687597 date "2006-01-01" @default.
• W409687597 modified "2023-09-23" @default.
• W409687597 title "Molecular diversity in chakkarakolli ( Gymnema sylvestre R. Br.) assessed through isozyme and RAPD analysis" @default.
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