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- W41328775 abstract "ABSTRACT BHK21 cells expressing a recombinant antibody were grown in a fixed bed bioreactor (FBR) containing Siran beads. The contribution of five process variables (bead and inoculum sizes; circulation and dilution rates; feed glutamine concentration) to process productivity were investigated in a partial factorial experiment. Individually, none of the variables tested had a significant affect upon productivity. The combination of smaller bead and inoculum sizes, higher circulation and dilution rates, plus higher feed glutamine concentration gave a markedly higher productivity than any other combination of variables tested. The data show the advantage of immobilisation culture over conventional low density batch techniques (e.g. microcarriers) for increasing specific and volumetric productivity." @default.
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- W41328775 date "1994-01-01" @default.
- W41328775 modified "2023-09-23" @default.
- W41328775 title "DEVELOPMENT OF A BIOREACTOR PROCESS FOR RECOMBINANT CELL LINES" @default.
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- W41328775 doi "https://doi.org/10.1016/b978-0-7506-1845-8.50060-0" @default.
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