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- W41505298 abstract "Some critical parameters such as the pumping system, mixing devices and detector design in instrumentation for post-column derivatization in high-performance liquid chromatography are discussed. The derivatization was studied with pharmaceutically important nona-peptides containing primary amino groups which react with Fluram® and reaction parameters such as pH, solvent and reagent concentration were investigated. Both adsorption systems and reversed-phase systems were used to separate the peptides prior to the post-column reaction. Reversed-phase chromatography has the advantage of simpler sample preparation, better reaction control and optimization of solvent conditions. As a result, detection limits of between 5 and 10 ng per injection can be obtained and the reproducibility of the results is better than ±2% (relative standard deviation). The method has been applied to the analysis of injection solutions (ampoules)." @default.
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- W41505298 date "1976-11-01" @default.
- W41505298 modified "2023-10-07" @default.
- W41505298 title "Post-column fluorescence derivatization of peptides" @default.
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- W41505298 doi "https://doi.org/10.1016/s0021-9673(01)84110-8" @default.
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