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• W41649474 abstract "Tamoxifen (TAM) is extensively used for the treatment and prevention of breast cancer. Associated with TAM treatment is a two- to eightfold increase in risk of endometrial cancer. To understand the mechanisms associated with this increased risk several pathways for TAM metabolism and DNA adduct formation have been studied. The purpose of this study was to investigate the role of peroxidase enzymes in the metabolism of TAM and its activation to form DNA adducts. Using advanced tandem mass spectrometry we have investigated the peroxidase-mediated metabolism of TAM. Incubation of TAM with horseradish peroxidase (HRP) and H2O2 produced multiple metabolites. Electrospray ionization–MS/MS analysis of the metabolites demonstrated a peak at 301.3 m/z with daughter ions at 183.0, 166.9, 128.9, and 120.9 m/z, which identified the metabolite as metabolite E (ME). The levels of ME were significantly inhibited by the addition of ascorbic acid to the incubation mixture. Co-incubation of either TAM or ME and DNA with HRP and H2O2 produced three DNA adducts with a RAL of 1.97 ± 0.01 × 10− 7 and 8.45 ± 2.7 × 10− 7. Oxidation of ME with MnO2 produced metabolite E quinone methide (MEQM). Furthermore, incubation of either TAM or ME with HRP and H2O2 resulted in formation of MEQM. Reaction of calf thymus DNA with MEQM produced three DNA adducts with a RAL of 9.8 ± 1.0 × 10− 7. Rechromatography analyses indicated that DNA adducts 1, 2, and 3 formed in the HRP activation of either TAM or ME were the same as those formed by the chemical reaction of DNA with MEQM. The results of these studies demonstrate that peroxidase enzymes can both metabolize TAM to form the primary metabolite ME and activate ME to a quinone methide intermediate, which reacts with DNA to form adducts. It is possible that peroxidase enzymes or peroxidase-like activity in endometrium could contribute to the formation of DNA damage and genotoxic effects in endometrium after TAM administration." @default.
• W41649474 created "2016-06-24" @default.
• W41649474 creator A5022821570 @default.
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• W41649474 date "2012-01-01" @default.
• W41649474 modified "2023-10-13" @default.
• W41649474 title "Peroxidase-mediated dealkylation of tamoxifen, detected by electrospray ionization–mass spectrometry, and activation to form DNA adducts" @default.
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• W41649474 doi "https://doi.org/10.1016/j.freeradbiomed.2011.10.433" @default.
• W41649474 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3253372" @default.
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