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- W41743907 abstract "Rapid advancements in low-light-level imaging technology have made it possible to apply the fundamental principles of fluorescence spectroscopic analyses to living cells. The quantitation of an optical signal from living cells attached to a substrate is very difficult. Living cells move, change shape, and vary the local number density of membrane-bound organelles. Fluorescence ratio imaging provides a means of overcoming many of the problems associated with quantifying the complex temporal and spatial dynamics of molecules and organelles in living cells. This chapter describes the issues of fluorescence ratio imaging microscopy, including basic requirements of the instrumentation, validation of the basic tenets of ratioing as applied to microscopy, and some methods and issues in the application of ratio imaging. With continued development and improvement in fluorescent probes for ion measurements as well as the development of probes for other important molecules, the potential applicability of ratio imaging is enormous. Fluorescence ratio imaging is a spectroscopic technique and is usable for any technique that can be expressed by the relationship of signals at two or more wavelengths." @default.
- W41743907 created "2016-06-24" @default.
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- W41743907 date "1989-01-01" @default.
- W41743907 modified "2023-09-25" @default.
- W41743907 title "Chapter 6 Fluorescence Ratio Imaging Microscopy" @default.
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- W41743907 doi "https://doi.org/10.1016/s0091-679x(08)60979-6" @default.
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