Matches in SemOpenAlex for { <https://semopenalex.org/work/W41807374> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W41807374 endingPage "83" @default.
- W41807374 startingPage "1975" @default.
- W41807374 abstract "To investigate the involvement of polyamines (putrescine, spermidine, and spermine) in the proliferation of retinal pigment epithelium (RPE) using cultured bovine RPE cells.The polyamine content and the activities of rate-limiting enzymes in polyamine biosynthesis (ornithine decarboxylase [ODC] and S-adenosylmethionine decarboxylase [SAMDC]) and in polyamine biodegradation (spermidine spermine N1-acetyltransferase [SAT]) were measured after proliferative stimulation by 10% fetal calf serum (FCS). DNA synthesis was determined by [3H]thymidine incorporation into the acid-insoluble fraction after the addition of an inhibitor of ODC (alpha-difluoromethylornithine [DFMO]) or SAMDC (methylglyoxal bis[guanylhydrazone] [MGBG]). The effects of exogenous polyamines on DNA synthesis after the additions of inhibitors also were determined.ODC and SAMDC activities were elevated after stimulation by FCS and reached their peaks 16 hours and 4 hours, respectively, after the addition of FCS. SAT activity was not increased. Polyamine content was increased significantly after stimulation by FCS. DFMO did not inhibit DNA synthesis induced by FCS, and only putrescine content was decreased significantly among polyamines. However, MGBG inhibited DNA synthesis in a dose-dependent manner, and the amounts of spermidine and spermine were decreased significantly. Exogenous polyamines, especially spermine, restored MGBG-induced inhibition of DNA synthesis.Polyamines are essential for the proliferation of cultured bovine RPE cells. These data suggest that, of the polyamines, spermine has the greatest effect on DNA synthesis although other polyamines can substitute for spermine at higher concentrations with similar results. As for polyamine metabolism in RPE proliferation, it is possible that SAMDC is the key enzyme rather than ODC." @default.
- W41807374 created "2016-06-24" @default.
- W41807374 creator A5027180020 @default.
- W41807374 creator A5037710621 @default.
- W41807374 creator A5063197214 @default.
- W41807374 creator A5076833685 @default.
- W41807374 creator A5087240649 @default.
- W41807374 date "1996-09-01" @default.
- W41807374 modified "2023-09-23" @default.
- W41807374 title "The involvement of polyamines in the proliferation of cultured retinal pigment epithelial cells." @default.
- W41807374 cites W1481162800 @default.
- W41807374 cites W1496546960 @default.
- W41807374 cites W1513354202 @default.
- W41807374 cites W1556979365 @default.
- W41807374 cites W1783564941 @default.
- W41807374 cites W184029216 @default.
- W41807374 cites W1844699750 @default.
- W41807374 cites W1958737571 @default.
- W41807374 cites W1975043689 @default.
- W41807374 cites W1976211275 @default.
- W41807374 cites W1987684676 @default.
- W41807374 cites W2007465467 @default.
- W41807374 cites W2012456950 @default.
- W41807374 cites W2020562013 @default.
- W41807374 cites W2033856946 @default.
- W41807374 cites W2054950434 @default.
- W41807374 cites W2059377012 @default.
- W41807374 cites W2060702039 @default.
- W41807374 cites W2083729530 @default.
- W41807374 cites W2087905322 @default.
- W41807374 cites W2146424420 @default.
- W41807374 cites W2161356660 @default.
- W41807374 cites W2281124822 @default.
- W41807374 cites W2344044870 @default.
- W41807374 cites W2345067921 @default.
- W41807374 cites W2469489813 @default.
- W41807374 cites W17299339 @default.
- W41807374 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8814137" @default.
- W41807374 hasPublicationYear "1996" @default.
- W41807374 type Work @default.
- W41807374 sameAs 41807374 @default.
- W41807374 citedByCount "8" @default.
- W41807374 countsByYear W418073742017 @default.
- W41807374 countsByYear W418073742022 @default.
- W41807374 crossrefType "journal-article" @default.
- W41807374 hasAuthorship W41807374A5027180020 @default.
- W41807374 hasAuthorship W41807374A5037710621 @default.
- W41807374 hasAuthorship W41807374A5063197214 @default.
- W41807374 hasAuthorship W41807374A5076833685 @default.
- W41807374 hasAuthorship W41807374A5087240649 @default.
- W41807374 hasConcept C121289238 @default.
- W41807374 hasConcept C153911025 @default.
- W41807374 hasConcept C181199279 @default.
- W41807374 hasConcept C2776109719 @default.
- W41807374 hasConcept C2777167505 @default.
- W41807374 hasConcept C2777497464 @default.
- W41807374 hasConcept C2780115381 @default.
- W41807374 hasConcept C552990157 @default.
- W41807374 hasConcept C55493867 @default.
- W41807374 hasConcept C64241487 @default.
- W41807374 hasConcept C86803240 @default.
- W41807374 hasConceptScore W41807374C121289238 @default.
- W41807374 hasConceptScore W41807374C153911025 @default.
- W41807374 hasConceptScore W41807374C181199279 @default.
- W41807374 hasConceptScore W41807374C2776109719 @default.
- W41807374 hasConceptScore W41807374C2777167505 @default.
- W41807374 hasConceptScore W41807374C2777497464 @default.
- W41807374 hasConceptScore W41807374C2780115381 @default.
- W41807374 hasConceptScore W41807374C552990157 @default.
- W41807374 hasConceptScore W41807374C55493867 @default.
- W41807374 hasConceptScore W41807374C64241487 @default.
- W41807374 hasConceptScore W41807374C86803240 @default.
- W41807374 hasIssue "10" @default.
- W41807374 hasLocation W418073741 @default.
- W41807374 hasOpenAccess W41807374 @default.
- W41807374 hasPrimaryLocation W418073741 @default.
- W41807374 hasRelatedWork W1892225957 @default.
- W41807374 hasRelatedWork W1949110994 @default.
- W41807374 hasRelatedWork W1976211275 @default.
- W41807374 hasRelatedWork W2034854973 @default.
- W41807374 hasRelatedWork W2083729530 @default.
- W41807374 hasRelatedWork W2095594022 @default.
- W41807374 hasRelatedWork W2310739789 @default.
- W41807374 hasRelatedWork W3112532739 @default.
- W41807374 hasRelatedWork W325004197 @default.
- W41807374 hasRelatedWork W41807374 @default.
- W41807374 hasVolume "37" @default.
- W41807374 isParatext "false" @default.
- W41807374 isRetracted "false" @default.
- W41807374 magId "41807374" @default.
- W41807374 workType "article" @default.