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- W419954883 abstract "Bacterial resistance, arising from biofilm production is the most commonproblem facing in osteomyelitis, especially when implant is involved.Here, we aim to find a solution for this problem by testing the new fusion ofGentamicin Sulfate and Nigella sativa oil in emulsion form against biofilmproducing strain microorganism. A fusion of Gentamicin-N.sativa formulatedin the form of water-in-oil (w/o) and oil-in-water (o/w) emulsion. The finalconcentration of N.sativa oil varied from 32.5% (v/v) to 46.6% (v/v) and Gentamicinconcentration was constant with 1% (w/v). After measurement ofemulsion parameters (organoleptic characteristics, pH, particle size, differentstorage temperature) finished and passed stability and stress testing, theywere subjected for Disc Diffusion Test to screen the effect against bacteria.Three species of bacteria used which were S.aureus (ATCC 29213),P.aeruginosa (ATCC 70888), and S.epidermidis (isolated from osteomyelitic patient).Biofilm formation abilities was confirmed and assessed usingthe Microtitre Plate Method. S.epidermidis used in this study also has beenidentified as Gentamicin resistant. Briefly, 20µl of each emulsion, N.sativa oilalone, and 1% gentamicin loaded in blank discs (Oxoid) and placed onMueller Hinton Agar media that has been spread with bacteria previously.Later, all plates incubated at 37°C for 18 to 24 hours ±30 minutes. Mean diameterof the zone of inhibition measured after incubation and analyzedusing one way ANOVA for comparison between emulsions, 1% Gentamicin,and N.sativa oil alone. All four emulsions tested against S.aureus showedslightly higher zone of inhibition than 1% Gentamicin but only emulsion Dhas a p value 0.05, while on theP.aeruginosa the zone inhibition of emulsions showed slightly lower thanGentamicin with p value>0.05. For S.epidermidis, all emulsions showed significantlyhigher zone inhibition than Gentamicin with p value<0.05. AllN.sativa alone (with same percentage concentrations in each emulsion) producedan effect against S.aureus and S.epidermidis except for P.aeruginosa.However the effect was much lower compared to all emulsions and GentamicinSulfate. Even though the effect against S.aureus and P.aeruginosawere not significantly different compared to Gentamicin alone, surprisingly itis significantly effective against gentamicin resistant S.epidermidis" @default.
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- W419954883 date "2014-01-01" @default.
- W419954883 modified "2023-09-27" @default.
- W419954883 title "In vitro antimicrobial effect of Gentamicin-N.sativa fusion emulsion against biofilm producing strain S.aureus, P.aeruginosa and S.epidermidis" @default.
- W419954883 hasPublicationYear "2014" @default.
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