FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200001995> ?p ?o ?g. }

• W4200001995 endingPage "153903" @default.
• W4200001995 startingPage "153903" @default.
• W4200001995 abstract "Paeonol is the extractive of Paeonia suffruticosa Andr and is reported to reverse the chemotherapy resistance of cancer cells. The present study explores the role of paeonol in inhibiting the malignant biological behaviors of Apatinib-resistant gastric cancer (GC) cells.The Traditional Chinese Medicine Systems Pharmacology (TCMSP) database was adopted to screen the target genes of paeonol, and the STRING database was employed to construct a protein-protein interaction (PPI) network. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the target genes was performed employing DAVID online database. The expressions of these target genes in GC tissues and para-cancerous tissues were analyzed with GEPIA database, and GEO datasets (GSE109476 and GSE93415) were utilized to analyze differentially expressed lncRNAs and miRNAs in GC tissues and para-cancerous tissues. The expressions of LINC00665, miR-665 and MAPK1 mRNA in Apatinib-resistant GC cells were detected through quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay was conducted to detect cell proliferation; Transwell assays were employed to detect cell migration and invasion, and TdT-mediated dUTP nick end labeling (TUNEL) assay was utilized to detect cell apoptosis. Dual-luciferase reporter gene assay was performed to detect the binding relationships between miR-665 and LINC00665, as well as between miR-665 and MAPK1 mRNA. The expressions of MAPK1 protein and glycolysis-associated proteins (GLUT1, LDHB and HK2) were detected by Western blot. Additionally, a tumor xenograft mice model was constructed to evaluate the effects of paeonol on lung metastasis.Paeonol could inhibit the proliferation, migration, invasion and glycolysis, and promote the apoptosis of Apatinib-resistant GC cells. TCMSP database suggested that Paeonol had 17 target genes, and 17 target genes were mainly enriched in signaling pathways related to apoptosis, glucose and lipid metabolism, etc.; GEPIA database suggests that MAPK1, among the 17 target genes, was markedly elevated in GC tissues. Paeonol could decrease LINC00665 and MAPK1 expressions in GC cells but increase the expression of miR-665. LINC00665 overexpression, MAPK1 overexpression or inhibition of miR-665 could abolish the inhibitive effects of paeonol on the malignant phenotypes of Apatinib-resistant GC cells. miR-665 is verified as an upstream regulator of MAPK1 and a target of LINC00665. Additionally, paeonol could significantly inhibit the lung metastasis in the tumor xenograft mice model.Paeonol can inhibit the malignancy of Apatinib-resistant GC cells through LINC00665/miR-665/MAPK1 axis. For the first time, our study imply that paeonol may be a potential drug to reverse Apatinib-resistant of GC cells." @default.
• W4200001995 created "2021-12-31" @default.
• W4200001995 creator A5021384155 @default.
• W4200001995 creator A5050522599 @default.
• W4200001995 creator A5063797577 @default.
• W4200001995 creator A5083932301 @default.
• W4200001995 date "2022-02-01" @default.
• W4200001995 modified "2023-10-18" @default.
• W4200001995 title "Paeonol inhibits the malignancy of Apatinib-resistant gastric cancer cells via LINC00665/miR-665/MAPK1 axis" @default.
• W4200001995 cites W1792564133 @default.
• W4200001995 cites W1994355795 @default.
• W4200001995 cites W2005055360 @default.
• W4200001995 cites W2014100191 @default.
• W4200001995 cites W2015194118 @default.
• W4200001995 cites W2017107262 @default.
• W4200001995 cites W2025049828 @default.
• W4200001995 cites W2032183472 @default.
• W4200001995 cites W2106657141 @default.
• W4200001995 cites W2127441349 @default.
• W4200001995 cites W2133661134 @default.
• W4200001995 cites W2162674813 @default.
• W4200001995 cites W2292909024 @default.
• W4200001995 cites W2591601567 @default.
• W4200001995 cites W2605473687 @default.
• W4200001995 cites W2742535624 @default.
• W4200001995 cites W2755349124 @default.
• W4200001995 cites W2767432511 @default.
• W4200001995 cites W2770249785 @default.
• W4200001995 cites W2806187310 @default.
• W4200001995 cites W2889646458 @default.
• W4200001995 cites W2904668328 @default.
• W4200001995 cites W2921628265 @default.
• W4200001995 cites W2941184640 @default.
• W4200001995 cites W2953559774 @default.
• W4200001995 cites W2970460399 @default.
• W4200001995 cites W2971462397 @default.
• W4200001995 cites W2977484339 @default.
• W4200001995 cites W2987812036 @default.
• W4200001995 cites W3011159162 @default.
• W4200001995 cites W3024490705 @default.
• W4200001995 cites W3083928251 @default.
• W4200001995 cites W3093283000 @default.
• W4200001995 cites W3103462716 @default.
• W4200001995 cites W3119039305 @default.
• W4200001995 cites W3123664110 @default.
• W4200001995 cites W3157871772 @default.
• W4200001995 cites W3163091683 @default.
• W4200001995 cites W3165166663 @default.
• W4200001995 doi "https://doi.org/10.1016/j.phymed.2021.153903" @default.
• W4200001995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35026514" @default.
• W4200001995 hasPublicationYear "2022" @default.
• W4200001995 type Work @default.
• W4200001995 citedByCount "13" @default.
• W4200001995 countsByYear W42000019952022 @default.
• W4200001995 countsByYear W42000019952023 @default.
• W4200001995 crossrefType "journal-article" @default.
• W4200001995 hasAuthorship W4200001995A5021384155 @default.
• W4200001995 hasAuthorship W4200001995A5050522599 @default.
• W4200001995 hasAuthorship W4200001995A5063797577 @default.
• W4200001995 hasAuthorship W4200001995A5083932301 @default.
• W4200001995 hasConcept C104317684 @default.
• W4200001995 hasConcept C121608353 @default.
• W4200001995 hasConcept C142724271 @default.
• W4200001995 hasConcept C145059251 @default.
• W4200001995 hasConcept C150194340 @default.
• W4200001995 hasConcept C152724338 @default.
• W4200001995 hasConcept C153911025 @default.
• W4200001995 hasConcept C162317418 @default.
• W4200001995 hasConcept C185592680 @default.
• W4200001995 hasConcept C204787440 @default.
• W4200001995 hasConcept C2778833331 @default.
• W4200001995 hasConcept C2780032014 @default.
• W4200001995 hasConcept C502942594 @default.
• W4200001995 hasConcept C54355233 @default.
• W4200001995 hasConcept C55493867 @default.
• W4200001995 hasConcept C62112901 @default.
• W4200001995 hasConcept C71924100 @default.
• W4200001995 hasConcept C86803240 @default.
• W4200001995 hasConcept C96232424 @default.
• W4200001995 hasConceptScore W4200001995C104317684 @default.
• W4200001995 hasConceptScore W4200001995C121608353 @default.
• W4200001995 hasConceptScore W4200001995C142724271 @default.
• W4200001995 hasConceptScore W4200001995C145059251 @default.
• W4200001995 hasConceptScore W4200001995C150194340 @default.
• W4200001995 hasConceptScore W4200001995C152724338 @default.
• W4200001995 hasConceptScore W4200001995C153911025 @default.
• W4200001995 hasConceptScore W4200001995C162317418 @default.
• W4200001995 hasConceptScore W4200001995C185592680 @default.
• W4200001995 hasConceptScore W4200001995C204787440 @default.
• W4200001995 hasConceptScore W4200001995C2778833331 @default.
• W4200001995 hasConceptScore W4200001995C2780032014 @default.
• W4200001995 hasConceptScore W4200001995C502942594 @default.
• W4200001995 hasConceptScore W4200001995C54355233 @default.
• W4200001995 hasConceptScore W4200001995C55493867 @default.
• W4200001995 hasConceptScore W4200001995C62112901 @default.
• W4200001995 hasConceptScore W4200001995C71924100 @default.
• W4200001995 hasConceptScore W4200001995C86803240 @default.
• W4200001995 hasConceptScore W4200001995C96232424 @default.

• next