FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200003102> ?p ?o ?g. }

• W4200003102 endingPage "7545" @default.
• W4200003102 startingPage "7545" @default.
• W4200003102 abstract "Acetes chinensis (belonging to the Decapoda Sergestidae genus) is widely distributed in East Asian waters and is extremely widespread and present in the shallow coastal areas of China. Polyphenol oxidase (PPO), which was extracted from Acetes chinensis, was purified in a four-step procedure involving phosphate-buffered saline treatment, ammonium sulphate precipitation, DEAE-Cellulose chromatography, and Phenyl-Sepharose HP chromatography, and then, its biochemical characterization was measured. The specific activity of the purified enzyme was increased to 643.4 U/mg, which is a 30.35 times increase in purification, and the recovery rate was 17.9%. L-dopa was used as the substrate, the enzymatic reactions catalyzed by PPO conformed to the Michaelis equation, the maximum reaction velocity was 769.23 U/mL, and the Michaelis constant Km was 0.846 mmol/L. The optimal pH of PPO from Acetes chinensis was 7.5, and the optimal temperature was 35 °C. The metal ions experiment showed that Mn2+ and K+ could enhance the activity of PPO; that Ba2+ and Ca2+ could inhibit the activity of PPO; and that Cu2+ had a double effect on PPO, increasing the PPO activity at low concentrations and inhibiting the PPO activity at high concentrations. The inhibitor experiment showed that the inhibitory effects of EDTA and kojic acid were weak and that ascorbic acid and sodium pyrophosphate had good inhibitory effects. The purification and characterization of Acetes chinensis serve as guidelines for the prediction of enzyme behavior, leading to effective prevention of enzymatic browning during processing." @default.
• W4200003102 created "2021-12-31" @default.
• W4200003102 creator A5017706961 @default.
• W4200003102 creator A5026374763 @default.
• W4200003102 creator A5048027490 @default.
• W4200003102 creator A5048111269 @default.
• W4200003102 creator A5065863457 @default.
• W4200003102 creator A5071264679 @default.
• W4200003102 creator A5075153959 @default.
• W4200003102 date "2021-12-13" @default.
• W4200003102 modified "2023-10-17" @default.
• W4200003102 title "Study on Purification and Characterization of Polyphenol Oxidase from Acetes chinensis" @default.
• W4200003102 cites W148402036 @default.
• W4200003102 cites W1837171585 @default.
• W4200003102 cites W1977382340 @default.
• W4200003102 cites W1978628024 @default.
• W4200003102 cites W1988219047 @default.
• W4200003102 cites W2007382701 @default.
• W4200003102 cites W2035662987 @default.
• W4200003102 cites W2035765149 @default.
• W4200003102 cites W2041346416 @default.
• W4200003102 cites W2050943171 @default.
• W4200003102 cites W2064208493 @default.
• W4200003102 cites W2092993321 @default.
• W4200003102 cites W2097006506 @default.
• W4200003102 cites W2116193314 @default.
• W4200003102 cites W2406036039 @default.
• W4200003102 cites W2791143337 @default.
• W4200003102 cites W2807564773 @default.
• W4200003102 cites W2880172662 @default.
• W4200003102 cites W2886971501 @default.
• W4200003102 cites W3034027657 @default.
• W4200003102 doi "https://doi.org/10.3390/molecules26247545" @default.
• W4200003102 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34946625" @default.
• W4200003102 hasPublicationYear "2021" @default.
• W4200003102 type Work @default.
• W4200003102 citedByCount "4" @default.
• W4200003102 countsByYear W42000031022022 @default.
• W4200003102 countsByYear W42000031022023 @default.
• W4200003102 crossrefType "journal-article" @default.
• W4200003102 hasAuthorship W4200003102A5017706961 @default.
• W4200003102 hasAuthorship W4200003102A5026374763 @default.
• W4200003102 hasAuthorship W4200003102A5048027490 @default.
• W4200003102 hasAuthorship W4200003102A5048111269 @default.
• W4200003102 hasAuthorship W4200003102A5065863457 @default.
• W4200003102 hasAuthorship W4200003102A5071264679 @default.
• W4200003102 hasAuthorship W4200003102A5075153959 @default.
• W4200003102 hasBestOaLocation W42000031021 @default.
• W4200003102 hasConcept C162008176 @default.
• W4200003102 hasConcept C181199279 @default.
• W4200003102 hasConcept C185592680 @default.
• W4200003102 hasConcept C18903297 @default.
• W4200003102 hasConcept C193363816 @default.
• W4200003102 hasConcept C2776043855 @default.
• W4200003102 hasConcept C2776338311 @default.
• W4200003102 hasConcept C2776428949 @default.
• W4200003102 hasConcept C2777132085 @default.
• W4200003102 hasConcept C2777289219 @default.
• W4200003102 hasConcept C2779538156 @default.
• W4200003102 hasConcept C28212737 @default.
• W4200003102 hasConcept C2986274086 @default.
• W4200003102 hasConcept C31903555 @default.
• W4200003102 hasConcept C43617362 @default.
• W4200003102 hasConcept C53007507 @default.
• W4200003102 hasConcept C55493867 @default.
• W4200003102 hasConcept C64915919 @default.
• W4200003102 hasConcept C86803240 @default.
• W4200003102 hasConcept C87554066 @default.
• W4200003102 hasConceptScore W4200003102C162008176 @default.
• W4200003102 hasConceptScore W4200003102C181199279 @default.
• W4200003102 hasConceptScore W4200003102C185592680 @default.
• W4200003102 hasConceptScore W4200003102C18903297 @default.
• W4200003102 hasConceptScore W4200003102C193363816 @default.
• W4200003102 hasConceptScore W4200003102C2776043855 @default.
• W4200003102 hasConceptScore W4200003102C2776338311 @default.
• W4200003102 hasConceptScore W4200003102C2776428949 @default.
• W4200003102 hasConceptScore W4200003102C2777132085 @default.
• W4200003102 hasConceptScore W4200003102C2777289219 @default.
• W4200003102 hasConceptScore W4200003102C2779538156 @default.
• W4200003102 hasConceptScore W4200003102C28212737 @default.
• W4200003102 hasConceptScore W4200003102C2986274086 @default.
• W4200003102 hasConceptScore W4200003102C31903555 @default.
• W4200003102 hasConceptScore W4200003102C43617362 @default.
• W4200003102 hasConceptScore W4200003102C53007507 @default.
• W4200003102 hasConceptScore W4200003102C55493867 @default.
• W4200003102 hasConceptScore W4200003102C64915919 @default.
• W4200003102 hasConceptScore W4200003102C86803240 @default.
• W4200003102 hasConceptScore W4200003102C87554066 @default.
• W4200003102 hasIssue "24" @default.
• W4200003102 hasLocation W42000031021 @default.
• W4200003102 hasLocation W42000031022 @default.
• W4200003102 hasLocation W42000031023 @default.
• W4200003102 hasLocation W42000031024 @default.
• W4200003102 hasOpenAccess W4200003102 @default.
• W4200003102 hasPrimaryLocation W42000031021 @default.
• W4200003102 hasRelatedWork W2060879534 @default.
• W4200003102 hasRelatedWork W2236862897 @default.
• W4200003102 hasRelatedWork W2280312886 @default.

• next