FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200009750> ?p ?o ?g. }

• W4200009750 endingPage "2839" @default.
• W4200009750 startingPage "2828" @default.
• W4200009750 abstract "Senecavirus A (SVA), a newly emergent picornavirus correlated with sudden neonatal mortality and vesicular lesions in pigs, has had a considerable impact on the global pig farming industry. Timely and dependable detection of SVA is helpful in preventing the further spread of this pathogenic virus. In the current study, a real-time fluorescent reverse transcription recombinase-aided amplification (rRT-RAA) assay, which targets the most conserved region within the VP2 gene of SVA, was developed and evaluated for SVA detection. The detection limit for this assay was tested to be 1.185 50% tissue culture infective dose (TCID50) of SVA RNA per reaction at a 95% confidence interval, which is comparable to that of a previously published rRT-PCR assay for SVA. The testing results of the rRT-RAA assay were very reproducible and repeatable, with inter- and intra-assay coefficient of variation values less than 7.0%. In addition, the established rRT-RAA assay displayed excellent specificity for SVA detection without cross-reaction with other clinically important swine pathogenic viruses. The diagnostic performance of rRT-RAA was evaluated using 189 clinical swine samples, which were detected in parallel using the reference rRT-PCR assay. The results showed that 146 and 151 samples tested positive for SVA by rRT-RAA and rRT-PCR, respectively. The overall agreement between both assays was 97.4% (184/189) with a kappa value of 0.927 (p < .001). Further linear regression analysis demonstrated that the detection results between the two assays were significantly correlated (R2 = 0.9192, p < .0001). Taken together, our newly established rRT-RAA assay is a powerful and time-saving diagnostic tool for SVA detection in clinical samples." @default.
• W4200009750 created "2021-12-31" @default.
• W4200009750 creator A5026104854 @default.
• W4200009750 creator A5031560516 @default.
• W4200009750 creator A5048277748 @default.
• W4200009750 creator A5049445988 @default.
• W4200009750 creator A5050388216 @default.
• W4200009750 creator A5058757625 @default.
• W4200009750 creator A5075052232 @default.
• W4200009750 creator A5085081570 @default.
• W4200009750 date "2022-01-06" @default.
• W4200009750 modified "2023-10-12" @default.
• W4200009750 title "Development of a VP2‐based real‐time fluorescent reverse transcription recombinase‐aided amplification assay to rapidly detect Senecavirus A" @default.
• W4200009750 cites W1909850880 @default.
• W4200009750 cites W2163194887 @default.
• W4200009750 cites W2165224832 @default.
• W4200009750 cites W2399194636 @default.
• W4200009750 cites W2414776849 @default.
• W4200009750 cites W2418450768 @default.
• W4200009750 cites W2465282427 @default.
• W4200009750 cites W2505039247 @default.
• W4200009750 cites W2517778016 @default.
• W4200009750 cites W2546020723 @default.
• W4200009750 cites W2550048650 @default.
• W4200009750 cites W2571483111 @default.
• W4200009750 cites W2605548524 @default.
• W4200009750 cites W2610633384 @default.
• W4200009750 cites W2610795704 @default.
• W4200009750 cites W2619110964 @default.
• W4200009750 cites W2626831984 @default.
• W4200009750 cites W2736010073 @default.
• W4200009750 cites W2883463319 @default.
• W4200009750 cites W2898663990 @default.
• W4200009750 cites W2899240216 @default.
• W4200009750 cites W2901510132 @default.
• W4200009750 cites W2969668991 @default.
• W4200009750 cites W2985431863 @default.
• W4200009750 cites W3005169663 @default.
• W4200009750 cites W3026508945 @default.
• W4200009750 cites W3031983426 @default.
• W4200009750 cites W3043866022 @default.
• W4200009750 cites W3088038985 @default.
• W4200009750 cites W3093388671 @default.
• W4200009750 cites W3138560468 @default.
• W4200009750 doi "https://doi.org/10.1111/tbed.14435" @default.
• W4200009750 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34931455" @default.
• W4200009750 hasPublicationYear "2022" @default.
• W4200009750 type Work @default.
• W4200009750 citedByCount "6" @default.
• W4200009750 countsByYear W42000097502022 @default.
• W4200009750 countsByYear W42000097502023 @default.
• W4200009750 crossrefType "journal-article" @default.
• W4200009750 hasAuthorship W4200009750A5026104854 @default.
• W4200009750 hasAuthorship W4200009750A5031560516 @default.
• W4200009750 hasAuthorship W4200009750A5048277748 @default.
• W4200009750 hasAuthorship W4200009750A5049445988 @default.
• W4200009750 hasAuthorship W4200009750A5050388216 @default.
• W4200009750 hasAuthorship W4200009750A5058757625 @default.
• W4200009750 hasAuthorship W4200009750A5075052232 @default.
• W4200009750 hasAuthorship W4200009750A5085081570 @default.
• W4200009750 hasBestOaLocation W42000097501 @default.
• W4200009750 hasConcept C104317684 @default.
• W4200009750 hasConcept C105580179 @default.
• W4200009750 hasConcept C119128265 @default.
• W4200009750 hasConcept C153911025 @default.
• W4200009750 hasConcept C156719811 @default.
• W4200009750 hasConcept C159047783 @default.
• W4200009750 hasConcept C185592680 @default.
• W4200009750 hasConcept C43617362 @default.
• W4200009750 hasConcept C4692481 @default.
• W4200009750 hasConcept C48023723 @default.
• W4200009750 hasConcept C54355233 @default.
• W4200009750 hasConcept C67705224 @default.
• W4200009750 hasConcept C86803240 @default.
• W4200009750 hasConceptScore W4200009750C104317684 @default.
• W4200009750 hasConceptScore W4200009750C105580179 @default.
• W4200009750 hasConceptScore W4200009750C119128265 @default.
• W4200009750 hasConceptScore W4200009750C153911025 @default.
• W4200009750 hasConceptScore W4200009750C156719811 @default.
• W4200009750 hasConceptScore W4200009750C159047783 @default.
• W4200009750 hasConceptScore W4200009750C185592680 @default.
• W4200009750 hasConceptScore W4200009750C43617362 @default.
• W4200009750 hasConceptScore W4200009750C4692481 @default.
• W4200009750 hasConceptScore W4200009750C48023723 @default.
• W4200009750 hasConceptScore W4200009750C54355233 @default.
• W4200009750 hasConceptScore W4200009750C67705224 @default.
• W4200009750 hasConceptScore W4200009750C86803240 @default.
• W4200009750 hasFunder F4320321001 @default.
• W4200009750 hasFunder F4320322919 @default.
• W4200009750 hasIssue "5" @default.
• W4200009750 hasLocation W42000097501 @default.
• W4200009750 hasLocation W42000097502 @default.
• W4200009750 hasOpenAccess W4200009750 @default.
• W4200009750 hasPrimaryLocation W42000097501 @default.
• W4200009750 hasRelatedWork W1512954123 @default.
• W4200009750 hasRelatedWork W172632108 @default.
• W4200009750 hasRelatedWork W1974846613 @default.
• W4200009750 hasRelatedWork W2119522412 @default.

• next