FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200012317> ?p ?o ?g. }

• W4200012317 endingPage "737813" @default.
• W4200012317 startingPage "737813" @default.
• W4200012317 abstract "Cherax quadricarinatus, commonly known as the redclaw crayfish, possesses enormous commercial benefits. To prevent germplasm degradation of C. quadricarinatus populations, there is an urgent need to protect its germplasm resources. One of the important links in the breeding of improved varieties is family selection. In this study, we screened for microsatellite markers based on the whole genome sequence of C. quadricarinatus. The obtained polymorphic tetranucleotide microsatellite genetic markers were combined with capillary electrophoresis to establish an experimental system suitable for parentage assignment of C. quadricarinatus. Sixty polymorphic tetrameric microsatellite markers with stable amplification were screened from the reference genome sequence of C. quadricarinatus. By continuously optimizing the annealing temperature, primer concentration, and cycle numbers of the combination, 20 tetranucleotide microsatellite loci with good amplification effect and a high polymorphism information content (PIC) were obtained. Multiplex PCR systems were then constructed using five groups of microsatellites. Parentage assignment in 12 families of C. quadricarinatus with known pedigree information was carried out using CERVUS 3.0 software. The results showed that 112 alleles were amplified from the 20 loci. The observed heterozygosity (Ho) ranged from 0.458 to 0.917. The expected heterozygosity (He) ranged from 0.435 to 0.832 The PIC ranged from 0.375 to 0.788. Most of the microsatellite markers were highly polymorphic and could meet the requirements for parentage assignment. Using any four combinations, the actual successful assignment rates ranged from 97.20% to 99.30%, and when using all five combinations, the assignment rate reached 100%. Therefore, selecting these sets could not only obtain accurate pedigree information but also would reduce the workload and cost. The microsatellite multiplex PCR system constructed in this study provides a convenient and useful method for molecular pedigree construction, population breeding, and family management of C. quadricarinatus, thus forming a basis to ensure the normal development of this important freshwater crustacean." @default.
• W4200012317 created "2021-12-31" @default.
• W4200012317 creator A5027866475 @default.
• W4200012317 creator A5037848240 @default.
• W4200012317 creator A5043245428 @default.
• W4200012317 creator A5043430437 @default.
• W4200012317 creator A5059497907 @default.
• W4200012317 creator A5068959729 @default.
• W4200012317 creator A5070654618 @default.
• W4200012317 creator A5072719298 @default.
• W4200012317 creator A5085083831 @default.
• W4200012317 date "2022-03-01" @default.
• W4200012317 modified "2023-09-23" @default.
• W4200012317 title "Development of a multiplex PCR assay for parentage assignment of the redclaw crayfish (Cherax quadricarinatus)" @default.
• W4200012317 cites W1187828627 @default.
• W4200012317 cites W1975805818 @default.
• W4200012317 cites W1980432461 @default.
• W4200012317 cites W1991028888 @default.
• W4200012317 cites W2011796376 @default.
• W4200012317 cites W2013749670 @default.
• W4200012317 cites W2015768575 @default.
• W4200012317 cites W2020867650 @default.
• W4200012317 cites W2020997435 @default.
• W4200012317 cites W2025281280 @default.
• W4200012317 cites W2025654359 @default.
• W4200012317 cites W2029141732 @default.
• W4200012317 cites W2029180509 @default.
• W4200012317 cites W2037614295 @default.
• W4200012317 cites W2043894736 @default.
• W4200012317 cites W2048471241 @default.
• W4200012317 cites W2048699035 @default.
• W4200012317 cites W2055625638 @default.
• W4200012317 cites W2059147403 @default.
• W4200012317 cites W2067248681 @default.
• W4200012317 cites W2073794964 @default.
• W4200012317 cites W2076451101 @default.
• W4200012317 cites W2084507708 @default.
• W4200012317 cites W2102221632 @default.
• W4200012317 cites W2107473082 @default.
• W4200012317 cites W2111517921 @default.
• W4200012317 cites W2130700168 @default.
• W4200012317 cites W2167287925 @default.
• W4200012317 cites W2487792753 @default.
• W4200012317 cites W2591683344 @default.
• W4200012317 cites W2765438221 @default.
• W4200012317 cites W2801471586 @default.
• W4200012317 cites W2885761637 @default.
• W4200012317 cites W2889931387 @default.
• W4200012317 cites W2943946720 @default.
• W4200012317 cites W2998795673 @default.
• W4200012317 cites W3015278401 @default.
• W4200012317 cites W3032928492 @default.
• W4200012317 cites W3041411073 @default.
• W4200012317 cites W3116343512 @default.
• W4200012317 cites W3173372183 @default.
• W4200012317 cites W3174339697 @default.
• W4200012317 cites W3176377679 @default.
• W4200012317 cites W3186512276 @default.
• W4200012317 cites W4249101476 @default.
• W4200012317 doi "https://doi.org/10.1016/j.aquaculture.2021.737813" @default.
• W4200012317 hasPublicationYear "2022" @default.
• W4200012317 type Work @default.
• W4200012317 citedByCount "3" @default.
• W4200012317 countsByYear W42000123172022 @default.
• W4200012317 countsByYear W42000123172023 @default.
• W4200012317 crossrefType "journal-article" @default.
• W4200012317 hasAuthorship W4200012317A5027866475 @default.
• W4200012317 hasAuthorship W4200012317A5037848240 @default.
• W4200012317 hasAuthorship W4200012317A5043245428 @default.
• W4200012317 hasAuthorship W4200012317A5043430437 @default.
• W4200012317 hasAuthorship W4200012317A5059497907 @default.
• W4200012317 hasAuthorship W4200012317A5068959729 @default.
• W4200012317 hasAuthorship W4200012317A5070654618 @default.
• W4200012317 hasAuthorship W4200012317A5072719298 @default.
• W4200012317 hasAuthorship W4200012317A5085083831 @default.
• W4200012317 hasConcept C104317684 @default.
• W4200012317 hasConcept C143589142 @default.
• W4200012317 hasConcept C180754005 @default.
• W4200012317 hasConcept C2777010711 @default.
• W4200012317 hasConcept C2777461220 @default.
• W4200012317 hasConcept C2781403440 @default.
• W4200012317 hasConcept C49105822 @default.
• W4200012317 hasConcept C505870484 @default.
• W4200012317 hasConcept C54355233 @default.
• W4200012317 hasConcept C59822182 @default.
• W4200012317 hasConcept C61320498 @default.
• W4200012317 hasConcept C86803240 @default.
• W4200012317 hasConceptScore W4200012317C104317684 @default.
• W4200012317 hasConceptScore W4200012317C143589142 @default.
• W4200012317 hasConceptScore W4200012317C180754005 @default.
• W4200012317 hasConceptScore W4200012317C2777010711 @default.
• W4200012317 hasConceptScore W4200012317C2777461220 @default.
• W4200012317 hasConceptScore W4200012317C2781403440 @default.
• W4200012317 hasConceptScore W4200012317C49105822 @default.
• W4200012317 hasConceptScore W4200012317C505870484 @default.
• W4200012317 hasConceptScore W4200012317C54355233 @default.
• W4200012317 hasConceptScore W4200012317C59822182 @default.
• W4200012317 hasConceptScore W4200012317C61320498 @default.

• next