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- W4200012318 abstract "• CD34 − cells impaired expansion ex vivo of CD34 + cells by TGF-β1. • TGF-β1 was accumulated during expansion ex vivo . • Full medium replacement offsets the infleuence of CD34 − cells on expansion of HSCs. Haematopoietic stem cells (HSCs) exist in CD34 + cells and lose CD34 marker expression when they differentiate into CD34 − cells during ex vivo expansion. In this work, periodical removal of CD34 − cells with full medium replacement and full medium replacement were performed to investigate the influence of CD34 − cells on the expansion of CD34 + cells. After 10 days of culture, the percentages of CD34 + cells with symmetric self-renewing division cultured according to these two strategies were significantly higher than those in the control group. As a result, by using these two strategies, the concentrations of total TGF-β1 and activated TGF-β1 were investigated and were significantly reduced compared with those in the control group. The expansion of CD34 + cells was significantly greater than that of control cells. Importantly, the expansion of CD34 + CD38 − CD45RA − CD49f + CD90 + cells was also significantly increased. Moreover, both of the two strategies better maintained the colony-forming ability and secondary expansion ability of cells. The qRT-PCR results further indicated that key genes involved in the self-renewal and function of HSCs were upregulated (pu.1, hoxa9, hoxb4, hes1 and bmi1 ). Therefore, we speculated that CD34 − cells inhibited the expansion of CD34 + cells by TGF-β1." @default.
- W4200012318 created "2021-12-31" @default.
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- W4200012318 date "2022-01-01" @default.
- W4200012318 modified "2023-09-27" @default.
- W4200012318 title "Removal of CD34- cells to increase self-renewal symmetric division and expansion ex vivo of cord blood CD34+ cells through reducing the TGF-β1" @default.
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- W4200012318 doi "https://doi.org/10.1016/j.procbio.2021.12.002" @default.
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