Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200030364> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W4200030364 abstract "Abstract Background: Polycystic ovary syndrome (PCOS), a common endocrinal disorder, is associated with impaired oocyte development, which leads to infertility. However, the pathogenesis of PCOS has not been completely elucidated. Limited studies have analyzed the pathological characteristics of oocytes in PCOS. This study aimed to analyze the differentially expressed genes (DEGs) and epigenetic changes in the oocytes of the PCOS mouse model to identify the etiological factors. Methods: C57BL/6J female mice were subcutaneously injected with vehicle or 5α-dihydrotestosterone (250 µg/day) on days 16–18 of pregnancy. Female offspring were used as the control or PCOS group. The oocytes were collected from mice aged 7–9 weeks. The DEGs between the control and PCOS groups were analyzed using RNA sequencing (RNA-Seq). Additionally, the DNA methylation status was analyzed using the post-bisulfite adaptor tagging method. The ovarian tissue sections were stained with hematoxylin and eosin to examine the morphological changes. The proteins, Rps21 and Rpl36, were measured using immunostaining. Results: Compared with the control group, the PCOS group exhibited impaired estrous cycle and polycystic ovary-like morphology. RNA-Seq analysis revealed that 90 DEGs were upregulated and 27 DEGs were downregulated in the PCOS mouse model. DNA methylation analysis revealed 30 hypomethylated and 10 hypermethylated regions in the PCOS group. However, the DNA methylation status was not correlated with differential gene expression. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis revealed that five DEGs ( Rps21 , Rpl36 , Rpl36a , Rpl37a , and Rpl22l1 ) were enriched in ribosome-related pathways. The immunohistochemical analysis revealed that the expression levels of Rps21 and Rpl36 were significantly upregulated in the PCOS mouse model. Conclusions: These results suggest that differential gene expression in the oocytes of the PCOS mouse model is related to impaired folliculogenesis. These findings improved our understanding of the pathogenesis of PCOS." @default.
- W4200030364 created "2021-12-31" @default.
- W4200030364 creator A5003815973 @default.
- W4200030364 creator A5004674567 @default.
- W4200030364 creator A5005963678 @default.
- W4200030364 creator A5009105996 @default.
- W4200030364 creator A5016411927 @default.
- W4200030364 creator A5028218533 @default.
- W4200030364 creator A5034893886 @default.
- W4200030364 creator A5049751858 @default.
- W4200030364 creator A5049909599 @default.
- W4200030364 creator A5054091248 @default.
- W4200030364 creator A5060979238 @default.
- W4200030364 creator A5065111458 @default.
- W4200030364 creator A5069870074 @default.
- W4200030364 creator A5070117387 @default.
- W4200030364 creator A5077956208 @default.
- W4200030364 creator A5078859785 @default.
- W4200030364 creator A5084930721 @default.
- W4200030364 creator A5085664919 @default.
- W4200030364 creator A5088688171 @default.
- W4200030364 creator A5090280925 @default.
- W4200030364 date "2021-12-13" @default.
- W4200030364 modified "2023-10-16" @default.
- W4200030364 title "Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Findings of Differential Gene Expression Analysis of Oocytes" @default.
- W4200030364 doi "https://doi.org/10.21203/rs.3.rs-1155132/v1" @default.
- W4200030364 hasPublicationYear "2021" @default.
- W4200030364 type Work @default.
- W4200030364 citedByCount "0" @default.
- W4200030364 crossrefType "posted-content" @default.
- W4200030364 hasAuthorship W4200030364A5003815973 @default.
- W4200030364 hasAuthorship W4200030364A5004674567 @default.
- W4200030364 hasAuthorship W4200030364A5005963678 @default.
- W4200030364 hasAuthorship W4200030364A5009105996 @default.
- W4200030364 hasAuthorship W4200030364A5016411927 @default.
- W4200030364 hasAuthorship W4200030364A5028218533 @default.
- W4200030364 hasAuthorship W4200030364A5034893886 @default.
- W4200030364 hasAuthorship W4200030364A5049751858 @default.
- W4200030364 hasAuthorship W4200030364A5049909599 @default.
- W4200030364 hasAuthorship W4200030364A5054091248 @default.
- W4200030364 hasAuthorship W4200030364A5060979238 @default.
- W4200030364 hasAuthorship W4200030364A5065111458 @default.
- W4200030364 hasAuthorship W4200030364A5069870074 @default.
- W4200030364 hasAuthorship W4200030364A5070117387 @default.
- W4200030364 hasAuthorship W4200030364A5077956208 @default.
- W4200030364 hasAuthorship W4200030364A5078859785 @default.
- W4200030364 hasAuthorship W4200030364A5084930721 @default.
- W4200030364 hasAuthorship W4200030364A5085664919 @default.
- W4200030364 hasAuthorship W4200030364A5088688171 @default.
- W4200030364 hasAuthorship W4200030364A5090280925 @default.
- W4200030364 hasBestOaLocation W42000303641 @default.
- W4200030364 hasConcept C104317684 @default.
- W4200030364 hasConcept C126322002 @default.
- W4200030364 hasConcept C134018914 @default.
- W4200030364 hasConcept C150194340 @default.
- W4200030364 hasConcept C16685009 @default.
- W4200030364 hasConcept C189235521 @default.
- W4200030364 hasConcept C190727270 @default.
- W4200030364 hasConcept C2777391703 @default.
- W4200030364 hasConcept C2779306644 @default.
- W4200030364 hasConcept C3018442814 @default.
- W4200030364 hasConcept C33288867 @default.
- W4200030364 hasConcept C41091548 @default.
- W4200030364 hasConcept C54355233 @default.
- W4200030364 hasConcept C71924100 @default.
- W4200030364 hasConcept C86803240 @default.
- W4200030364 hasConceptScore W4200030364C104317684 @default.
- W4200030364 hasConceptScore W4200030364C126322002 @default.
- W4200030364 hasConceptScore W4200030364C134018914 @default.
- W4200030364 hasConceptScore W4200030364C150194340 @default.
- W4200030364 hasConceptScore W4200030364C16685009 @default.
- W4200030364 hasConceptScore W4200030364C189235521 @default.
- W4200030364 hasConceptScore W4200030364C190727270 @default.
- W4200030364 hasConceptScore W4200030364C2777391703 @default.
- W4200030364 hasConceptScore W4200030364C2779306644 @default.
- W4200030364 hasConceptScore W4200030364C3018442814 @default.
- W4200030364 hasConceptScore W4200030364C33288867 @default.
- W4200030364 hasConceptScore W4200030364C41091548 @default.
- W4200030364 hasConceptScore W4200030364C54355233 @default.
- W4200030364 hasConceptScore W4200030364C71924100 @default.
- W4200030364 hasConceptScore W4200030364C86803240 @default.
- W4200030364 hasLocation W42000303641 @default.
- W4200030364 hasOpenAccess W4200030364 @default.
- W4200030364 hasPrimaryLocation W42000303641 @default.
- W4200030364 hasRelatedWork W2090160399 @default.
- W4200030364 hasRelatedWork W2113483234 @default.
- W4200030364 hasRelatedWork W2609822003 @default.
- W4200030364 hasRelatedWork W2769193000 @default.
- W4200030364 hasRelatedWork W2950845984 @default.
- W4200030364 hasRelatedWork W3004424038 @default.
- W4200030364 hasRelatedWork W3161067307 @default.
- W4200030364 hasRelatedWork W4281926227 @default.
- W4200030364 hasRelatedWork W4290860628 @default.
- W4200030364 hasRelatedWork W779414787 @default.
- W4200030364 isParatext "false" @default.
- W4200030364 isRetracted "false" @default.
- W4200030364 workType "article" @default.