FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200079630> ?p ?o ?g. }

• W4200079630 endingPage "679" @default.
• W4200079630 startingPage "667" @default.
• W4200079630 abstract "Intestinal fibrosis is a serious complication of Crohn's disease. Numerous cell types including intestinal epithelial and mesenchymal cells are implicated in this process, yet studies are hampered by the lack of personalized in vitro models. Human intestinal organoids (HIOs) derived from induced pluripotent stem cells (iPSCs) contain these cell types, and our goal was to determine the feasibility of utilizing these to develop a personalized intestinal fibrosis model.iPSCs from 2 control individuals and 2 very early onset inflammatory bowel disease patients with stricturing complications were obtained and directed to form HIOs. Purified populations of epithelial and mesenchymal cells were derived from HIOs, and both types were treated with the profibrogenic cytokine transforming growth factor β (TGFβ). Quantitative polymerase chain reaction and RNA sequencing analysis were used to assay their responses.In iPSC-derived mesenchymal cells, there was a significant increase in the expression of profibrotic genes (Col1a1, Col5a1, and TIMP1) in response to TGFβ. RNA sequencing analysis identified further profibrotic genes and demonstrated differential responses to this cytokine in each of the 4 lines. Increases in profibrotic gene expression (Col1a1, FN, TIMP1) along with genes associated with epithelial-mesenchymal transition (vimentin and N-cadherin) were observed in TGFβ -treated epithelial cells.We demonstrate the feasibility of utilizing iPSC-HIO technology to model intestinal fibrotic responses in vitro. This now permits the generation of near unlimited quantities of patient-specific cells that could be used to reveal cell- and environmental-specific mechanisms underpinning intestinal fibrosis.Intestinal fibrosis is a serious complication of Crohn’s disease and novel in vitro models are urgently needed to study this. We describe an induced pluripotent stem cell–derived modeling system whereby a near unlimited number of both epithelial and mesenchymal cells could be used in a personalized intestinal fibrosis model." @default.
• W4200079630 created "2021-12-31" @default.
• W4200079630 creator A5011814444 @default.
• W4200079630 creator A5022984619 @default.
• W4200079630 creator A5025393610 @default.
• W4200079630 creator A5051870878 @default.
• W4200079630 creator A5059599507 @default.
• W4200079630 creator A5088008120 @default.
• W4200079630 date "2021-12-17" @default.
• W4200079630 modified "2023-10-14" @default.
• W4200079630 title "Development of a Personalized Intestinal Fibrosis Model Using Human Intestinal Organoids Derived From Induced Pluripotent Stem Cells" @default.
• W4200079630 cites W1483496013 @default.
• W4200079630 cites W1968844364 @default.
• W4200079630 cites W1971411354 @default.
• W4200079630 cites W1973399209 @default.
• W4200079630 cites W1996139830 @default.
• W4200079630 cites W2002513358 @default.
• W4200079630 cites W2005224639 @default.
• W4200079630 cites W2010457001 @default.
• W4200079630 cites W2039427709 @default.
• W4200079630 cites W2075008712 @default.
• W4200079630 cites W2075594816 @default.
• W4200079630 cites W2093560642 @default.
• W4200079630 cites W2097795961 @default.
• W4200079630 cites W2106733239 @default.
• W4200079630 cites W2115865518 @default.
• W4200079630 cites W2130410032 @default.
• W4200079630 cites W2143296478 @default.
• W4200079630 cites W2143625465 @default.
• W4200079630 cites W2147832388 @default.
• W4200079630 cites W2154570785 @default.
• W4200079630 cites W2154589460 @default.
• W4200079630 cites W2159675211 @default.
• W4200079630 cites W2168056382 @default.
• W4200079630 cites W2169456326 @default.
• W4200079630 cites W2179438025 @default.
• W4200079630 cites W2206207244 @default.
• W4200079630 cites W2214074259 @default.
• W4200079630 cites W2529401211 @default.
• W4200079630 cites W2559823888 @default.
• W4200079630 cites W2565787913 @default.
• W4200079630 cites W2593695507 @default.
• W4200079630 cites W2602025036 @default.
• W4200079630 cites W2617856800 @default.
• W4200079630 cites W2780753528 @default.
• W4200079630 cites W2806928915 @default.
• W4200079630 cites W2912910216 @default.
• W4200079630 cites W2927147343 @default.
• W4200079630 cites W2928665623 @default.
• W4200079630 cites W2952240264 @default.
• W4200079630 cites W3004467012 @default.
• W4200079630 cites W3007355484 @default.
• W4200079630 cites W3042922031 @default.
• W4200079630 cites W3048264855 @default.
• W4200079630 cites W3082675235 @default.
• W4200079630 cites W3117944474 @default.
• W4200079630 cites W3119322979 @default.
• W4200079630 doi "https://doi.org/10.1093/ibd/izab292" @default.
• W4200079630 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34918082" @default.
• W4200079630 hasPublicationYear "2021" @default.
• W4200079630 type Work @default.
• W4200079630 citedByCount "6" @default.
• W4200079630 countsByYear W42000796302022 @default.
• W4200079630 countsByYear W42000796302023 @default.
• W4200079630 crossrefType "journal-article" @default.
• W4200079630 hasAuthorship W4200079630A5011814444 @default.
• W4200079630 hasAuthorship W4200079630A5022984619 @default.
• W4200079630 hasAuthorship W4200079630A5025393610 @default.
• W4200079630 hasAuthorship W4200079630A5051870878 @default.
• W4200079630 hasAuthorship W4200079630A5059599507 @default.
• W4200079630 hasAuthorship W4200079630A5088008120 @default.
• W4200079630 hasBestOaLocation W42000796301 @default.
• W4200079630 hasConcept C104317684 @default.
• W4200079630 hasConcept C107459253 @default.
• W4200079630 hasConcept C118131993 @default.
• W4200079630 hasConcept C127561419 @default.
• W4200079630 hasConcept C142724271 @default.
• W4200079630 hasConcept C145103041 @default.
• W4200079630 hasConcept C198826908 @default.
• W4200079630 hasConcept C2778260677 @default.
• W4200079630 hasConcept C2778615650 @default.
• W4200079630 hasConcept C2779134260 @default.
• W4200079630 hasConcept C2780559512 @default.
• W4200079630 hasConcept C28328180 @default.
• W4200079630 hasConcept C31695470 @default.
• W4200079630 hasConcept C502942594 @default.
• W4200079630 hasConcept C54355233 @default.
• W4200079630 hasConcept C71924100 @default.
• W4200079630 hasConcept C76419328 @default.
• W4200079630 hasConcept C86803240 @default.
• W4200079630 hasConcept C95444343 @default.
• W4200079630 hasConceptScore W4200079630C104317684 @default.
• W4200079630 hasConceptScore W4200079630C107459253 @default.
• W4200079630 hasConceptScore W4200079630C118131993 @default.
• W4200079630 hasConceptScore W4200079630C127561419 @default.
• W4200079630 hasConceptScore W4200079630C142724271 @default.
• W4200079630 hasConceptScore W4200079630C145103041 @default.
• W4200079630 hasConceptScore W4200079630C198826908 @default.

• next