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- W4200086136 abstract "Protein splicing is a self-catalyzed event where the intervening sequence intein cleaves off, joining the flanking exteins together to generate a functional protein. Attempts have been made to regulate the splicing rate through variations in temperature, pH, and metals. Although metal-regulated protein splicing has been more captivating to researchers, metals were shown to only inhibit splicing reactions that confine their application. This is the first study to show the effect of nanoparticles (NPs) on protein splicing. We found that gold nanoparticles (AuNPs) of various sizes can increase the splicing efficiency by more than 50% and the N-terminal cleavage efficiency by more than 45% in Mycobacterium tuberculosis SufB precursor protein. This study provides an effective strategy for engineering splicing-enhanced intein platforms. UV-vis absorption spectroscopy, isothermal titration calorimetry (ITC), and transmission electron microscopy (TEM) confirmed AuNP interaction with the native protein. Quantum mechanics/molecular mechanics (QM/MM) analysis suggested a significant reduction in the energy barrier at the N-terminal cleavage site in the presence of gold atom, strengthening our experimental evidence on heightened the N-terminal cleavage reaction. The encouraging observation of enhanced N-terminal cleavage and splicing reaction can have potential implementations from developing a rapid drug delivery system to designing a contemporary protein purification system." @default.
- W4200086136 created "2021-12-31" @default.
- W4200086136 creator A5003086112 @default.
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- W4200086136 date "2021-12-23" @default.
- W4200086136 modified "2023-09-25" @default.
- W4200086136 title "Gold Nanoparticles Augment N-Terminal Cleavage and Splicing Reactions in Mycobacterium tuberculosis SufB" @default.
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- W4200086136 cites W1963745089 @default.
- W4200086136 cites W1964788865 @default.
- W4200086136 cites W1966172363 @default.
- W4200086136 cites W1969814828 @default.
- W4200086136 cites W1971093707 @default.
- W4200086136 cites W1972490486 @default.
- W4200086136 cites W1989150705 @default.
- W4200086136 cites W1990799514 @default.
- W4200086136 cites W1993214561 @default.
- W4200086136 cites W1995748038 @default.
- W4200086136 cites W1998734880 @default.
- W4200086136 cites W2004785721 @default.
- W4200086136 cites W2004860860 @default.
- W4200086136 cites W2007151296 @default.
- W4200086136 cites W2008617358 @default.
- W4200086136 cites W2009695514 @default.
- W4200086136 cites W2011392342 @default.
- W4200086136 cites W2023631773 @default.
- W4200086136 cites W2025583051 @default.
- W4200086136 cites W2038246612 @default.
- W4200086136 cites W2042518528 @default.
- W4200086136 cites W2046405422 @default.
- W4200086136 cites W2048603130 @default.
- W4200086136 cites W2050195818 @default.
- W4200086136 cites W2051893857 @default.
- W4200086136 cites W2052509048 @default.
- W4200086136 cites W2054702374 @default.
- W4200086136 cites W2057497198 @default.
- W4200086136 cites W2059201273 @default.
- W4200086136 cites W2077190344 @default.
- W4200086136 cites W2079753165 @default.
- W4200086136 cites W2085889483 @default.
- W4200086136 cites W2088318300 @default.
- W4200086136 cites W2099626559 @default.
- W4200086136 cites W2106878780 @default.
- W4200086136 cites W2118771002 @default.
- W4200086136 cites W2119017543 @default.
- W4200086136 cites W2120636378 @default.
- W4200086136 cites W2124688926 @default.
- W4200086136 cites W2125451007 @default.
- W4200086136 cites W2134622589 @default.
- W4200086136 cites W2141980936 @default.
- W4200086136 cites W2159601544 @default.
- W4200086136 cites W2163542763 @default.
- W4200086136 cites W2165899507 @default.
- W4200086136 cites W2195807401 @default.
- W4200086136 cites W2254250178 @default.
- W4200086136 cites W2323152708 @default.
- W4200086136 cites W2333767757 @default.
- W4200086136 cites W2515340361 @default.
- W4200086136 cites W2528431469 @default.
- W4200086136 cites W2550436781 @default.
- W4200086136 cites W2565568481 @default.
- W4200086136 cites W2567409419 @default.
- W4200086136 cites W2604406610 @default.
- W4200086136 cites W2778862248 @default.
- W4200086136 cites W2793407638 @default.
- W4200086136 cites W2806349086 @default.
- W4200086136 cites W2913604863 @default.
- W4200086136 cites W2963838146 @default.
- W4200086136 cites W2972147604 @default.
- W4200086136 cites W2972223714 @default.
- W4200086136 cites W2973944376 @default.
- W4200086136 cites W2979812786 @default.
- W4200086136 cites W2984821377 @default.
- W4200086136 cites W3011302014 @default.
- W4200086136 cites W3026613319 @default.
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- W4200086136 cites W3068239004 @default.
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- W4200086136 cites W3195752113 @default.
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- W4200086136 doi "https://doi.org/10.3389/fbioe.2021.773303" @default.
- W4200086136 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35004641" @default.
- W4200086136 hasPublicationYear "2021" @default.
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