FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200112008> ?p ?o ?g. }

• W4200112008 abstract "Maturation of megakaryocytes culminates with extensive membrane rearrangements necessary for proplatelet formation. Mechanisms required for proplatelet extension and origin of membranes are still poorly understood. GTPase Rab5 (Ras-related protein in brain 5) regulates endocytic uptake and homotypic fusion of early endosomes and regulates phosphatidylinositol 3-monophosphate production important for binding of effector proteins during early-to-late endosomal/lysosomal maturation. Approach and Results: To investigate the role of Rab5 in megakaryocytes, we expressed GFP (green fluorescent protein)-coupled Rab5 wild type and its point mutants Q79L (active) and N133L (inactive) in primary murine fetal liver-derived megakaryocytes. Active Rab5 Q79L induced the formation of enlarged early endosomes, while inactive Rab5 N133L caused endosomal fragmentation. Consistently, an increased amount of transferrin internalization in Rab5 Q79L was impaired in Rab5 N133L expressing megakaryocytes, when compared with GFP or Rab5 wild type. Moreover, trafficking of GPIbβ (glycoprotein Ib subunit beta), a subunit of major megakaryocytes receptor and membrane marker, was found to be mediated by Rab5 activity. While GPIbβ was mostly present along the plasma membrane, and within cytoplasmic vesicles in Rab5 wild type megakaryocytes, it accumulated in the majority of Rab5 Q79L enlarged endosomes. Conversely, Rab5 N133L caused mostly GPIbβ plasma membrane retention. Furthermore, Rab5 Q79L expression increased incorporation of the membrane dye (PKH26), indicating higher membrane content. Finally, while Rab5 Q79L increased proplatelet production, inactive Rab5 N133L strongly inhibited it and was coupled with a decrease in late endosomes/lysosomes. Localization of GPIbβ in enlarged endosomes was phosphatidylinositol 3-monophosphate dependent.Taken together, our results demonstrate that Rab5-dependent endocytosis plays an important role in megakaryocytes receptor trafficking, membrane formation, and thrombopoiesis." @default.
• W4200112008 created "2021-12-31" @default.
• W4200112008 creator A5033084245 @default.
• W4200112008 creator A5035352256 @default.
• W4200112008 creator A5042919698 @default.
• W4200112008 creator A5080935014 @default.
• W4200112008 date "2022-01-01" @default.
• W4200112008 modified "2023-10-03" @default.
• W4200112008 title "Early Endosomal GTPase Rab5 (Ras-Related Protein in Brain 5) Regulates GPIbβ (Glycoprotein Ib Subunit β) Trafficking and Platelet Production In Vitro" @default.
• W4200112008 cites W1486436311 @default.
• W4200112008 cites W1492619899 @default.
• W4200112008 cites W1524150501 @default.
• W4200112008 cites W1606338704 @default.
• W4200112008 cites W1623300751 @default.
• W4200112008 cites W1967182910 @default.
• W4200112008 cites W1967467568 @default.
• W4200112008 cites W1970465522 @default.
• W4200112008 cites W1971124929 @default.
• W4200112008 cites W1977212437 @default.
• W4200112008 cites W1995558193 @default.
• W4200112008 cites W2000912956 @default.
• W4200112008 cites W2005574904 @default.
• W4200112008 cites W2007797957 @default.
• W4200112008 cites W2025094539 @default.
• W4200112008 cites W2034403065 @default.
• W4200112008 cites W2042640342 @default.
• W4200112008 cites W2044695265 @default.
• W4200112008 cites W2046133662 @default.
• W4200112008 cites W2049153875 @default.
• W4200112008 cites W2052438467 @default.
• W4200112008 cites W2059203800 @default.
• W4200112008 cites W2060891467 @default.
• W4200112008 cites W2110491903 @default.
• W4200112008 cites W2111383481 @default.
• W4200112008 cites W2114056096 @default.
• W4200112008 cites W2124781973 @default.
• W4200112008 cites W2138158580 @default.
• W4200112008 cites W2149491013 @default.
• W4200112008 cites W2150528960 @default.
• W4200112008 cites W2153457817 @default.
• W4200112008 cites W2168210128 @default.
• W4200112008 cites W2184724935 @default.
• W4200112008 cites W2269921753 @default.
• W4200112008 cites W2278040979 @default.
• W4200112008 cites W2323022101 @default.
• W4200112008 cites W2401610472 @default.
• W4200112008 cites W2410466081 @default.
• W4200112008 cites W2788865076 @default.
• W4200112008 cites W2889339510 @default.
• W4200112008 cites W2894458381 @default.
• W4200112008 cites W2904226286 @default.
• W4200112008 cites W2943885246 @default.
• W4200112008 cites W2949269903 @default.
• W4200112008 cites W2966791111 @default.
• W4200112008 cites W3005610176 @default.
• W4200112008 cites W3006574934 @default.
• W4200112008 doi "https://doi.org/10.1161/atvbaha.121.316552" @default.
• W4200112008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34732055" @default.
• W4200112008 hasPublicationYear "2022" @default.
• W4200112008 type Work @default.
• W4200112008 citedByCount "0" @default.
• W4200112008 crossrefType "journal-article" @default.
• W4200112008 hasAuthorship W4200112008A5033084245 @default.
• W4200112008 hasAuthorship W4200112008A5035352256 @default.
• W4200112008 hasAuthorship W4200112008A5042919698 @default.
• W4200112008 hasAuthorship W4200112008A5080935014 @default.
• W4200112008 hasBestOaLocation W42001120081 @default.
• W4200112008 hasConcept C102747710 @default.
• W4200112008 hasConcept C163181125 @default.
• W4200112008 hasConcept C170493617 @default.
• W4200112008 hasConcept C185592680 @default.
• W4200112008 hasConcept C2780298669 @default.
• W4200112008 hasConcept C2780610907 @default.
• W4200112008 hasConcept C28005876 @default.
• W4200112008 hasConcept C55493867 @default.
• W4200112008 hasConcept C62478195 @default.
• W4200112008 hasConcept C79747257 @default.
• W4200112008 hasConcept C79879829 @default.
• W4200112008 hasConcept C80631254 @default.
• W4200112008 hasConcept C86803240 @default.
• W4200112008 hasConcept C95444343 @default.
• W4200112008 hasConceptScore W4200112008C102747710 @default.
• W4200112008 hasConceptScore W4200112008C163181125 @default.
• W4200112008 hasConceptScore W4200112008C170493617 @default.
• W4200112008 hasConceptScore W4200112008C185592680 @default.
• W4200112008 hasConceptScore W4200112008C2780298669 @default.
• W4200112008 hasConceptScore W4200112008C2780610907 @default.
• W4200112008 hasConceptScore W4200112008C28005876 @default.
• W4200112008 hasConceptScore W4200112008C55493867 @default.
• W4200112008 hasConceptScore W4200112008C62478195 @default.
• W4200112008 hasConceptScore W4200112008C79747257 @default.
• W4200112008 hasConceptScore W4200112008C79879829 @default.
• W4200112008 hasConceptScore W4200112008C80631254 @default.
• W4200112008 hasConceptScore W4200112008C86803240 @default.
• W4200112008 hasConceptScore W4200112008C95444343 @default.
• W4200112008 hasIssue "1" @default.
• W4200112008 hasLocation W42001120081 @default.
• W4200112008 hasLocation W42001120082 @default.
• W4200112008 hasOpenAccess W4200112008 @default.
• W4200112008 hasPrimaryLocation W42001120081 @default.

• next