Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200156788> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W4200156788 abstract "The processing of most eukaryotic RNAs is mediated by RNA Binding Proteins (RBPs) with modular configurations, including an RNA recognition module, which specifically binds the pre-mRNA target and an effector domain. Previously, we have taken advantage of the unique RNA binding mode of the PUF domain in human Pumilio 1 to generate a programmable RNA binding scaffold, which was used to engineer various artificial RBPs to manipulate RNA metabolism. Here, a detailed protocol is described to construct Engineered Splicing Factors (ESFs) that are specifically designed to modulate the alternative splicing of target genes. The protocol includes how to design and construct a customized PUF scaffold for a specific RNA target, how to construct an ESF expression plasmid by fusing a designer PUF domain and an effector domain, and how to use ESFs to manipulate the splicing of target genes. In the representative results of this method, we have also described the common assays of ESF activities using splicing reporters, the application of ESF in cultured human cells, and the subsequent effect of splicing changes. By following the detailed protocols in this report, it is possible to design and generate ESFs for the regulation of different types of Alternative Splicing (AS), providing a new strategy to study splicing regulation and the function of different splicing isoforms. Moreover, by fusing different functional domains with a designed PUF domain, researchers can engineer artificial factors that target specific RNAs to manipulate various steps of RNA processing." @default.
- W4200156788 created "2021-12-31" @default.
- W4200156788 creator A5016526105 @default.
- W4200156788 creator A5019570332 @default.
- W4200156788 creator A5032069607 @default.
- W4200156788 creator A5035752408 @default.
- W4200156788 creator A5044702072 @default.
- W4200156788 creator A5059173157 @default.
- W4200156788 creator A5066797937 @default.
- W4200156788 date "2017-04-26" @default.
- W4200156788 modified "2023-09-26" @default.
- W4200156788 title "Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells" @default.
- W4200156788 doi "https://doi.org/10.3791/54967-v" @default.
- W4200156788 hasPublicationYear "2017" @default.
- W4200156788 type Work @default.
- W4200156788 citedByCount "0" @default.
- W4200156788 crossrefType "journal-article" @default.
- W4200156788 hasAuthorship W4200156788A5016526105 @default.
- W4200156788 hasAuthorship W4200156788A5019570332 @default.
- W4200156788 hasAuthorship W4200156788A5032069607 @default.
- W4200156788 hasAuthorship W4200156788A5035752408 @default.
- W4200156788 hasAuthorship W4200156788A5044702072 @default.
- W4200156788 hasAuthorship W4200156788A5059173157 @default.
- W4200156788 hasAuthorship W4200156788A5066797937 @default.
- W4200156788 hasBestOaLocation W42001567882 @default.
- W4200156788 hasConcept C104317684 @default.
- W4200156788 hasConcept C194583182 @default.
- W4200156788 hasConcept C41008148 @default.
- W4200156788 hasConcept C41282012 @default.
- W4200156788 hasConcept C51785407 @default.
- W4200156788 hasConcept C53345823 @default.
- W4200156788 hasConcept C54355233 @default.
- W4200156788 hasConcept C54458228 @default.
- W4200156788 hasConcept C67705224 @default.
- W4200156788 hasConcept C70721500 @default.
- W4200156788 hasConcept C86803240 @default.
- W4200156788 hasConcept C95444343 @default.
- W4200156788 hasConceptScore W4200156788C104317684 @default.
- W4200156788 hasConceptScore W4200156788C194583182 @default.
- W4200156788 hasConceptScore W4200156788C41008148 @default.
- W4200156788 hasConceptScore W4200156788C41282012 @default.
- W4200156788 hasConceptScore W4200156788C51785407 @default.
- W4200156788 hasConceptScore W4200156788C53345823 @default.
- W4200156788 hasConceptScore W4200156788C54355233 @default.
- W4200156788 hasConceptScore W4200156788C54458228 @default.
- W4200156788 hasConceptScore W4200156788C67705224 @default.
- W4200156788 hasConceptScore W4200156788C70721500 @default.
- W4200156788 hasConceptScore W4200156788C86803240 @default.
- W4200156788 hasConceptScore W4200156788C95444343 @default.
- W4200156788 hasIssue "122" @default.
- W4200156788 hasLocation W42001567881 @default.
- W4200156788 hasLocation W42001567882 @default.
- W4200156788 hasOpenAccess W4200156788 @default.
- W4200156788 hasPrimaryLocation W42001567881 @default.
- W4200156788 hasRelatedWork W1965544475 @default.
- W4200156788 hasRelatedWork W2009988114 @default.
- W4200156788 hasRelatedWork W2019097736 @default.
- W4200156788 hasRelatedWork W2040481343 @default.
- W4200156788 hasRelatedWork W2519557303 @default.
- W4200156788 hasRelatedWork W2609020171 @default.
- W4200156788 hasRelatedWork W2980949872 @default.
- W4200156788 hasRelatedWork W3119298630 @default.
- W4200156788 hasRelatedWork W3159376976 @default.
- W4200156788 hasRelatedWork W4225636447 @default.
- W4200156788 isParatext "false" @default.
- W4200156788 isRetracted "false" @default.
- W4200156788 workType "article" @default.