FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200175047> ?p ?o ?g. }

• W4200175047 endingPage "315" @default.
• W4200175047 startingPage "309" @default.
• W4200175047 abstract "ERG (ETS-related gene) is a member of the ETS (Erythroblast-transformation specific) family of transcription factors abundantly present in vascular endothelial cells. Recent studies demonstrate that ERG has important roles in blood vessel stability and angiogenesis. However, it is unclear how ERG is potentially involved in microvascular barrier functions and permeability. A wide variety of diseases and clinical conditions including trauma-hemorrhagic shock and burn injury are associated with microvascular dysfunctions, which causes excessive microvascular permeability, tissue edema and eventually, multiple organ dysfunction and death. The main purpose of this study was to determine the specific role of ERG in regulating microvascular permeability in human lung microvascular endothelial cells (HLMEC) and to evaluate if exogenous ERG will protect the barrier. The HLMECs were grown on Transwell inserts as monolayers and were transfected with ERG CRISPR/cas9 knockdown plasmid, ERG CRISPR activation plasmid, recombinant ERG protein or their respective controls. Recombinant vascular endothelial growth factor (VEGF) was used as an inducer of permeability for evaluating the effect of ERG activation on permeability. Changes in barrier integrity and permeability were studied using monolayer permeability assay and immunofluorescence of adherens junction proteins (VE-cadherin and β-catenin) respectively. CRISPR/cas9-based ERG knockdown as well as VEGF treatment induced monolayer hyperpermeability, VE-cadherin, and β-catenin junctional relocation and cytoskeletal F-actin stress fiber formation. CRISPR based ERG activation and recombinant ERG transfection attenuated VEGF-induced monolayer hyperpermeability. ERG activation preserved the adherens junctions and cytoskeleton. These results demonstrate that ERG is a potent regulator of barrier integrity and permeability in human lung microvascular endothelial cells and endogenously or exogenously enhancing ERG provides protection against barrier dysfunction and hyperpermeability." @default.
• W4200175047 created "2021-12-31" @default.
• W4200175047 creator A5004932139 @default.
• W4200175047 creator A5031156347 @default.
• W4200175047 creator A5036563034 @default.
• W4200175047 creator A5048202059 @default.
• W4200175047 creator A5064442125 @default.
• W4200175047 creator A5079551824 @default.
• W4200175047 creator A5086935268 @default.
• W4200175047 creator A5088873086 @default.
• W4200175047 date "2021-12-14" @default.
• W4200175047 modified "2023-10-16" @default.
• W4200175047 title "ETS-Related Gene Activation Preserves Adherens Junctions and Permeability in Microvascular Endothelial Cells" @default.
• W4200175047 cites W1510702369 @default.
• W4200175047 cites W1968129433 @default.
• W4200175047 cites W1981099729 @default.
• W4200175047 cites W1982771426 @default.
• W4200175047 cites W1991437268 @default.
• W4200175047 cites W1992855266 @default.
• W4200175047 cites W2002599138 @default.
• W4200175047 cites W2006057301 @default.
• W4200175047 cites W2013410789 @default.
• W4200175047 cites W2013647571 @default.
• W4200175047 cites W2028561648 @default.
• W4200175047 cites W2029742826 @default.
• W4200175047 cites W2029970671 @default.
• W4200175047 cites W2030902335 @default.
• W4200175047 cites W2045832625 @default.
• W4200175047 cites W2052728963 @default.
• W4200175047 cites W2064051725 @default.
• W4200175047 cites W2069493137 @default.
• W4200175047 cites W2075559846 @default.
• W4200175047 cites W2080899458 @default.
• W4200175047 cites W2081242900 @default.
• W4200175047 cites W2095315676 @default.
• W4200175047 cites W2099436620 @default.
• W4200175047 cites W2116398821 @default.
• W4200175047 cites W2128378329 @default.
• W4200175047 cites W2131316990 @default.
• W4200175047 cites W2146590089 @default.
• W4200175047 cites W2168471784 @default.
• W4200175047 cites W2325006147 @default.
• W4200175047 cites W2556613135 @default.
• W4200175047 doi "https://doi.org/10.1097/shk.0000000000001899" @default.
• W4200175047 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34907119" @default.
• W4200175047 hasPublicationYear "2021" @default.
• W4200175047 type Work @default.
• W4200175047 citedByCount "2" @default.
• W4200175047 countsByYear W42001750472022 @default.
• W4200175047 countsByYear W42001750472023 @default.
• W4200175047 crossrefType "journal-article" @default.
• W4200175047 hasAuthorship W4200175047A5004932139 @default.
• W4200175047 hasAuthorship W4200175047A5031156347 @default.
• W4200175047 hasAuthorship W4200175047A5036563034 @default.
• W4200175047 hasAuthorship W4200175047A5048202059 @default.
• W4200175047 hasAuthorship W4200175047A5064442125 @default.
• W4200175047 hasAuthorship W4200175047A5079551824 @default.
• W4200175047 hasAuthorship W4200175047A5086935268 @default.
• W4200175047 hasAuthorship W4200175047A5088873086 @default.
• W4200175047 hasBestOaLocation W42001750471 @default.
• W4200175047 hasConcept C103796816 @default.
• W4200175047 hasConcept C113045295 @default.
• W4200175047 hasConcept C120882062 @default.
• W4200175047 hasConcept C134018914 @default.
• W4200175047 hasConcept C1491633281 @default.
• W4200175047 hasConcept C149402561 @default.
• W4200175047 hasConcept C153911025 @default.
• W4200175047 hasConcept C177779419 @default.
• W4200175047 hasConcept C185592680 @default.
• W4200175047 hasConcept C201508349 @default.
• W4200175047 hasConcept C2780827179 @default.
• W4200175047 hasConcept C41625074 @default.
• W4200175047 hasConcept C55493867 @default.
• W4200175047 hasConcept C60903277 @default.
• W4200175047 hasConcept C86803240 @default.
• W4200175047 hasConcept C95444343 @default.
• W4200175047 hasConceptScore W4200175047C103796816 @default.
• W4200175047 hasConceptScore W4200175047C113045295 @default.
• W4200175047 hasConceptScore W4200175047C120882062 @default.
• W4200175047 hasConceptScore W4200175047C134018914 @default.
• W4200175047 hasConceptScore W4200175047C1491633281 @default.
• W4200175047 hasConceptScore W4200175047C149402561 @default.
• W4200175047 hasConceptScore W4200175047C153911025 @default.
• W4200175047 hasConceptScore W4200175047C177779419 @default.
• W4200175047 hasConceptScore W4200175047C185592680 @default.
• W4200175047 hasConceptScore W4200175047C201508349 @default.
• W4200175047 hasConceptScore W4200175047C2780827179 @default.
• W4200175047 hasConceptScore W4200175047C41625074 @default.
• W4200175047 hasConceptScore W4200175047C55493867 @default.
• W4200175047 hasConceptScore W4200175047C60903277 @default.
• W4200175047 hasConceptScore W4200175047C86803240 @default.
• W4200175047 hasConceptScore W4200175047C95444343 @default.
• W4200175047 hasIssue "2" @default.
• W4200175047 hasLocation W42001750471 @default.
• W4200175047 hasLocation W42001750472 @default.
• W4200175047 hasLocation W42001750473 @default.
• W4200175047 hasOpenAccess W4200175047 @default.
• W4200175047 hasPrimaryLocation W42001750471 @default.

• next