Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200180630> ?p ?o ?g. }
- W4200180630 endingPage "313" @default.
- W4200180630 startingPage "304" @default.
- W4200180630 abstract "Abstract Glycoengineering ultimately allows control over glycosylation patterns to generate new glycoprotein variants with desired properties. A common challenge is glycan heterogeneity, which may affect protein function and limit the use of key techniques such as mass spectrometry. Moreover, heterologous protein expression can introduce nonnative glycan chains that may not fulfill the requirement for therapeutic proteins. One strategy to address these challenges is partial trimming or complete removal of glycan chains, which can be obtained through selective application of exoglycosidases. Here, we demonstrate an enzymatic O-deglycosylation toolbox of a GH92 α-1,2-mannosidase from Neobacillus novalis, a GH2 β-galactofuranosidase from Amesia atrobrunnea and the jack bean α-mannosidase. The extent of enzymatic O-deglycosylation was mapped against a full glycosyl linkage analysis of the O-glycosylated linker of cellobiohydrolase I from Trichoderma reesei (TrCel7A). Furthermore, the influence of deglycosylation on TrCel7A functionality was evaluated by kinetic characterization of native and O-deglycosylated forms of TrCel7A. This study expands structural knowledge on fungal O-glycosylation and presents a ready-to-use enzymatic approach for controlled O-glycan engineering in glycoproteins expressed in filamentous fungi." @default.
- W4200180630 created "2021-12-31" @default.
- W4200180630 creator A5004784721 @default.
- W4200180630 creator A5009855971 @default.
- W4200180630 creator A5017699427 @default.
- W4200180630 creator A5019864741 @default.
- W4200180630 creator A5026750734 @default.
- W4200180630 creator A5029641676 @default.
- W4200180630 creator A5051189158 @default.
- W4200180630 creator A5069124324 @default.
- W4200180630 creator A5073577112 @default.
- W4200180630 date "2021-12-22" @default.
- W4200180630 modified "2023-10-17" @default.
- W4200180630 title "Analysis of fungal high-mannose structures using CAZymes" @default.
- W4200180630 cites W1824560409 @default.
- W4200180630 cites W1918970836 @default.
- W4200180630 cites W1935210339 @default.
- W4200180630 cites W1966678026 @default.
- W4200180630 cites W1967961992 @default.
- W4200180630 cites W1978096145 @default.
- W4200180630 cites W1985252740 @default.
- W4200180630 cites W2010791127 @default.
- W4200180630 cites W2029341292 @default.
- W4200180630 cites W2034969165 @default.
- W4200180630 cites W2038048096 @default.
- W4200180630 cites W2039021127 @default.
- W4200180630 cites W2049470922 @default.
- W4200180630 cites W2060579262 @default.
- W4200180630 cites W2073946352 @default.
- W4200180630 cites W2077005247 @default.
- W4200180630 cites W2090690774 @default.
- W4200180630 cites W2095114323 @default.
- W4200180630 cites W2122559203 @default.
- W4200180630 cites W2126695355 @default.
- W4200180630 cites W2136117026 @default.
- W4200180630 cites W2152105333 @default.
- W4200180630 cites W2156041732 @default.
- W4200180630 cites W2164112310 @default.
- W4200180630 cites W2190199302 @default.
- W4200180630 cites W2569540328 @default.
- W4200180630 cites W2621435706 @default.
- W4200180630 cites W2737828698 @default.
- W4200180630 cites W2754475126 @default.
- W4200180630 cites W2774296474 @default.
- W4200180630 cites W2897755148 @default.
- W4200180630 cites W2921437380 @default.
- W4200180630 cites W2947508095 @default.
- W4200180630 cites W2953110573 @default.
- W4200180630 cites W2985712209 @default.
- W4200180630 cites W3021893454 @default.
- W4200180630 cites W3047499802 @default.
- W4200180630 cites W3081528442 @default.
- W4200180630 cites W2976226488 @default.
- W4200180630 doi "https://doi.org/10.1093/glycob/cwab127" @default.
- W4200180630 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34939126" @default.
- W4200180630 hasPublicationYear "2021" @default.
- W4200180630 type Work @default.
- W4200180630 citedByCount "1" @default.
- W4200180630 countsByYear W42001806302023 @default.
- W4200180630 crossrefType "journal-article" @default.
- W4200180630 hasAuthorship W4200180630A5004784721 @default.
- W4200180630 hasAuthorship W4200180630A5009855971 @default.
- W4200180630 hasAuthorship W4200180630A5017699427 @default.
- W4200180630 hasAuthorship W4200180630A5019864741 @default.
- W4200180630 hasAuthorship W4200180630A5026750734 @default.
- W4200180630 hasAuthorship W4200180630A5029641676 @default.
- W4200180630 hasAuthorship W4200180630A5051189158 @default.
- W4200180630 hasAuthorship W4200180630A5069124324 @default.
- W4200180630 hasAuthorship W4200180630A5073577112 @default.
- W4200180630 hasBestOaLocation W42001806302 @default.
- W4200180630 hasConcept C104317684 @default.
- W4200180630 hasConcept C106686826 @default.
- W4200180630 hasConcept C108625454 @default.
- W4200180630 hasConcept C141603559 @default.
- W4200180630 hasConcept C161019410 @default.
- W4200180630 hasConcept C181199279 @default.
- W4200180630 hasConcept C185592680 @default.
- W4200180630 hasConcept C206212055 @default.
- W4200180630 hasConcept C2775887612 @default.
- W4200180630 hasConcept C2776125036 @default.
- W4200180630 hasConcept C2777313579 @default.
- W4200180630 hasConcept C2780115692 @default.
- W4200180630 hasConcept C55493867 @default.
- W4200180630 hasConcept C70721500 @default.
- W4200180630 hasConcept C86803240 @default.
- W4200180630 hasConceptScore W4200180630C104317684 @default.
- W4200180630 hasConceptScore W4200180630C106686826 @default.
- W4200180630 hasConceptScore W4200180630C108625454 @default.
- W4200180630 hasConceptScore W4200180630C141603559 @default.
- W4200180630 hasConceptScore W4200180630C161019410 @default.
- W4200180630 hasConceptScore W4200180630C181199279 @default.
- W4200180630 hasConceptScore W4200180630C185592680 @default.
- W4200180630 hasConceptScore W4200180630C206212055 @default.
- W4200180630 hasConceptScore W4200180630C2775887612 @default.
- W4200180630 hasConceptScore W4200180630C2776125036 @default.
- W4200180630 hasConceptScore W4200180630C2777313579 @default.
- W4200180630 hasConceptScore W4200180630C2780115692 @default.
- W4200180630 hasConceptScore W4200180630C55493867 @default.