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• W4200181703 endingPage "108783" @default.
• W4200181703 startingPage "108783" @default.
• W4200181703 abstract "Fungal spoilage of food commodities is still of great concern for food industry due to increased costs regarding food waste and product recalls. Traditional culture-based methods are laborious and require huge amounts of reagents and media. In addition, results are available after seven days rendering these techniques unsuitable for the intense production that exists nowadays. As a result, there is a need for faster and more sensitive methods for fungal detection. The aim of this study was the development and evaluation of a method (enrichment, sample treatment, DNA extraction, and qPCR) for the fast detection of spoilage fungi in fruit preparations. In this sense, a set of universal primers was selected and a hydrolysis probe was designed in-house. In addition, a non-competitive internal amplification control was included in the assay to eliminate false negative results due to reaction inhibition. It was demonstrated that the method could reliably detect yeasts with a LOD95 of 1.0 CFU/50 g. Regarding moulds detection, two different enrichment times were examined, namely 24 h and 48 h in order to increase the sensitivity. The obtained LOD95 values were 123.5 spores/50 g and 37.1 spores/50 g for 24 h and 48 h respectively. During the method evaluation all the performance parameters resulted in values higher than 85.0% and the Cohen's k was determined to be above 0.86 for yeasts and moulds. Overall, a reliable and sensitive next-day detection method for fungi was achieved." @default.
• W4200181703 created "2021-12-31" @default.
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• W4200181703 date "2022-05-01" @default.
• W4200181703 modified "2023-09-24" @default.
• W4200181703 title "Development of a real-time PCR assay with an internal amplification control for the detection of spoilage fungi in fruit preparations" @default.
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• W4200181703 doi "https://doi.org/10.1016/j.foodcont.2021.108783" @default.
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