Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200185390> ?p ?o ?g. }
Showing items 1 to 57 of
57
with 100 items per page.
- W4200185390 abstract "Background Hepatocellular carcinoma (HCC) is one of the most common malignancies around the world. Among the risk factors involved in liver carcinogenesis, hepatitis B virus (HBV) X protein (HBx) is considered to be a key regulator in hepatocarcinogenesis. Whether HBx promotes or protects against HCC remains controversial, therefore exploring new HBx-associated genes is still important. Methods HBx was overexpressed in HepG2, HepG2.2.15 and SMMC-7721 cell lines, primary mouse hepatocytes and livers of C57BL/6N mice. High-throughput RNA sequencing profiling of HepG2 cells with HBx overexpression and related differentially-expressed genes (DEGs), pathway enrichment analysis, protein-protein interaction networks (PPIs), overlapping analysis were conducted. In addition, Gene Expression Omnibus (GEO) and proteomic datasets of HBV-positive HCC datasets were used to verify the expression and prognosis of selected DEGs. Finally, we also evaluated the known oncogenic role of HBx by oncogenic array analysis. Results A total of 523 DEGs were obtained from HBx-overexpressing HepG2 cells. Twelve DEGs were identified and validated in cells transiently transfected with HBx and three datasets of HBV-positive HCC transcription profiles. In addition, using the Kaplan-Meier plotter database, the expression levels of the twelve different genes were further analyzed to predict patient outcomes. Conclusion Among the 12 identified HBx-associated hub genes, HBV-positive HCC patients expressing ARG1 and TAT showed a good overall survival (OS) and relapse-free survival (RFS). Thus, ARG1 and TAT expression could be potential prognostic markers." @default.
- W4200185390 created "2021-12-31" @default.
- W4200185390 date "2021-12-22" @default.
- W4200185390 modified "2023-09-24" @default.
- W4200185390 title "Table 1: List of primers used in the quantitative real-time PCR analysis." @default.
- W4200185390 doi "https://doi.org/10.7717/peerj.12697/table-1" @default.
- W4200185390 hasPublicationYear "2021" @default.
- W4200185390 type Work @default.
- W4200185390 citedByCount "0" @default.
- W4200185390 crossrefType "component" @default.
- W4200185390 hasBestOaLocation W42001853901 @default.
- W4200185390 hasConcept C104317684 @default.
- W4200185390 hasConcept C145059251 @default.
- W4200185390 hasConcept C150194340 @default.
- W4200185390 hasConcept C159047783 @default.
- W4200185390 hasConcept C18431079 @default.
- W4200185390 hasConcept C2522874641 @default.
- W4200185390 hasConcept C2778019345 @default.
- W4200185390 hasConcept C2780593183 @default.
- W4200185390 hasConcept C502942594 @default.
- W4200185390 hasConcept C54009773 @default.
- W4200185390 hasConcept C54355233 @default.
- W4200185390 hasConcept C555283112 @default.
- W4200185390 hasConcept C67567795 @default.
- W4200185390 hasConcept C70721500 @default.
- W4200185390 hasConcept C86803240 @default.
- W4200185390 hasConceptScore W4200185390C104317684 @default.
- W4200185390 hasConceptScore W4200185390C145059251 @default.
- W4200185390 hasConceptScore W4200185390C150194340 @default.
- W4200185390 hasConceptScore W4200185390C159047783 @default.
- W4200185390 hasConceptScore W4200185390C18431079 @default.
- W4200185390 hasConceptScore W4200185390C2522874641 @default.
- W4200185390 hasConceptScore W4200185390C2778019345 @default.
- W4200185390 hasConceptScore W4200185390C2780593183 @default.
- W4200185390 hasConceptScore W4200185390C502942594 @default.
- W4200185390 hasConceptScore W4200185390C54009773 @default.
- W4200185390 hasConceptScore W4200185390C54355233 @default.
- W4200185390 hasConceptScore W4200185390C555283112 @default.
- W4200185390 hasConceptScore W4200185390C67567795 @default.
- W4200185390 hasConceptScore W4200185390C70721500 @default.
- W4200185390 hasConceptScore W4200185390C86803240 @default.
- W4200185390 hasLocation W42001853901 @default.
- W4200185390 hasOpenAccess W4200185390 @default.
- W4200185390 hasPrimaryLocation W42001853901 @default.
- W4200185390 hasRelatedWork W10389219 @default.
- W4200185390 hasRelatedWork W10894809 @default.
- W4200185390 hasRelatedWork W11954045 @default.
- W4200185390 hasRelatedWork W12597883 @default.
- W4200185390 hasRelatedWork W5890245 @default.
- W4200185390 hasRelatedWork W6688127 @default.
- W4200185390 hasRelatedWork W7598718 @default.
- W4200185390 hasRelatedWork W8023415 @default.
- W4200185390 hasRelatedWork W8851756 @default.
- W4200185390 hasRelatedWork W942930 @default.
- W4200185390 isParatext "false" @default.
- W4200185390 isRetracted "false" @default.
- W4200185390 workType "paratext" @default.