Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200201552> ?p ?o ?g. }
- W4200201552 endingPage "133817" @default.
- W4200201552 startingPage "133817" @default.
- W4200201552 abstract "Residual dipolar couplings (RDCs) are widely used in NMR for the investigation of protein structure and dynamics. However, commonly used RDC media (e.g., C12E5/Hexanol/H2O or PEG system, lipid bilayer or bicelle system) are unstable viscous liquids, making it challenging to obtain stable and homogeneous NMR samples by laborious manual operation. In this paper, we report a unique fishhook-shaped micromixer (FS-mixer) that enables fast and thorough mixing of high-viscosity PEG and bicelle media for RDC analysis. Numerical simulations and experimental characterizations proved that the micromixer could achieve a complete mixing of viscous liquids in 382.4 μs, improved significantly over current mixers. In compared to manually prepared RDC samples, the on-chip prepared samples have a better uniformity and alignment efficiency, and show more stable peak split in the 2H spectrum. As a proof of concept, we prepared a RDC sample for INSM1 ZF1-2 protein by using this FS-mixer. The IPAP-HSQC spectra demonstrated that this strategy can efficiently prevent the generation of low resolution and distorted spectra, which could facilitate further refinement of the NMR structure. This micromixer-assisted sampling method greatly simplifies the procedure, increases the media uniformity and stability, and shortens the time in RDC analysis. We believe it could serve as a useful tool in RDC-based NMR analysis." @default.
- W4200201552 created "2021-12-31" @default.
- W4200201552 creator A5004372181 @default.
- W4200201552 creator A5010815895 @default.
- W4200201552 creator A5016279721 @default.
- W4200201552 creator A5031546626 @default.
- W4200201552 creator A5058166806 @default.
- W4200201552 creator A5059547902 @default.
- W4200201552 creator A5061462119 @default.
- W4200201552 creator A5080902615 @default.
- W4200201552 date "2022-03-01" @default.
- W4200201552 modified "2023-10-17" @default.
- W4200201552 title "A fast microfluidic mixer enabling rapid preparation of homogeneous PEG and bicelle media for RDC in NMR analysis" @default.
- W4200201552 cites W1970172422 @default.
- W4200201552 cites W1974049708 @default.
- W4200201552 cites W1980139576 @default.
- W4200201552 cites W1980284892 @default.
- W4200201552 cites W1983029383 @default.
- W4200201552 cites W1994328955 @default.
- W4200201552 cites W1997477098 @default.
- W4200201552 cites W1997592310 @default.
- W4200201552 cites W2002749442 @default.
- W4200201552 cites W2026908430 @default.
- W4200201552 cites W2031988904 @default.
- W4200201552 cites W2045515495 @default.
- W4200201552 cites W2048197073 @default.
- W4200201552 cites W2049181488 @default.
- W4200201552 cites W2060191139 @default.
- W4200201552 cites W2068078209 @default.
- W4200201552 cites W2069993625 @default.
- W4200201552 cites W2074333690 @default.
- W4200201552 cites W2076987726 @default.
- W4200201552 cites W2083904584 @default.
- W4200201552 cites W2090189932 @default.
- W4200201552 cites W2091222361 @default.
- W4200201552 cites W2093968495 @default.
- W4200201552 cites W2106325419 @default.
- W4200201552 cites W2112103709 @default.
- W4200201552 cites W2143825685 @default.
- W4200201552 cites W2144731691 @default.
- W4200201552 cites W2157566126 @default.
- W4200201552 cites W2159332812 @default.
- W4200201552 cites W2160679445 @default.
- W4200201552 cites W2169821755 @default.
- W4200201552 cites W2173561299 @default.
- W4200201552 cites W2192893032 @default.
- W4200201552 cites W2315441895 @default.
- W4200201552 cites W2323082545 @default.
- W4200201552 cites W2330328827 @default.
- W4200201552 cites W2469104722 @default.
- W4200201552 cites W2565082047 @default.
- W4200201552 cites W2583473528 @default.
- W4200201552 cites W2743797640 @default.
- W4200201552 cites W2783489435 @default.
- W4200201552 cites W2811045808 @default.
- W4200201552 cites W2918279612 @default.
- W4200201552 cites W2944575036 @default.
- W4200201552 cites W2957799231 @default.
- W4200201552 cites W2994898963 @default.
- W4200201552 cites W3035828639 @default.
- W4200201552 cites W3043241433 @default.
- W4200201552 cites W3099943579 @default.
- W4200201552 doi "https://doi.org/10.1016/j.cej.2021.133817" @default.
- W4200201552 hasPublicationYear "2022" @default.
- W4200201552 type Work @default.
- W4200201552 citedByCount "3" @default.
- W4200201552 countsByYear W42002015522023 @default.
- W4200201552 crossrefType "journal-article" @default.
- W4200201552 hasAuthorship W4200201552A5004372181 @default.
- W4200201552 hasAuthorship W4200201552A5010815895 @default.
- W4200201552 hasAuthorship W4200201552A5016279721 @default.
- W4200201552 hasAuthorship W4200201552A5031546626 @default.
- W4200201552 hasAuthorship W4200201552A5058166806 @default.
- W4200201552 hasAuthorship W4200201552A5059547902 @default.
- W4200201552 hasAuthorship W4200201552A5061462119 @default.
- W4200201552 hasAuthorship W4200201552A5080902615 @default.
- W4200201552 hasConcept C10138342 @default.
- W4200201552 hasConcept C103319777 @default.
- W4200201552 hasConcept C113196181 @default.
- W4200201552 hasConcept C121332964 @default.
- W4200201552 hasConcept C1276947 @default.
- W4200201552 hasConcept C138777275 @default.
- W4200201552 hasConcept C162324750 @default.
- W4200201552 hasConcept C171250308 @default.
- W4200201552 hasConcept C178790620 @default.
- W4200201552 hasConcept C185592680 @default.
- W4200201552 hasConcept C192562407 @default.
- W4200201552 hasConcept C2780982322 @default.
- W4200201552 hasConcept C43617362 @default.
- W4200201552 hasConcept C4839761 @default.
- W4200201552 hasConcept C54400483 @default.
- W4200201552 hasConcept C62520636 @default.
- W4200201552 hasConcept C67787023 @default.
- W4200201552 hasConcept C8673954 @default.
- W4200201552 hasConceptScore W4200201552C10138342 @default.
- W4200201552 hasConceptScore W4200201552C103319777 @default.
- W4200201552 hasConceptScore W4200201552C113196181 @default.
- W4200201552 hasConceptScore W4200201552C121332964 @default.