FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200205329> ?p ?o ?g. }

• W4200205329 abstract "Abstract Background In ischemic stroke, the function of the cerebral vasculature is impaired. This vascular structure is formed by the so-called neurovascular unit (NVU). A better understanding of the mechanisms involved in NVU dysfunction and recovery may lead to new insights for the development of highly sought therapeutic approaches. To date, there remains an unmet need for complex human in vitro models of the NVU to study ischemic events seen in the human brain. Methods We here describe the development of a human NVU on-a-chip model using a platform that allows culture of 40 chips in parallel. The model comprises a perfused vessel of primary human brain endothelial cells in co-culture with induced pluripotent stem cell derived astrocytes and neurons. Ischemic stroke was mimicked using a threefold approach that combines chemical hypoxia, hypoglycemia, and halted perfusion. Results Immunofluorescent staining confirmed expression of endothelial adherens and tight junction proteins, as well as astrocytic and neuronal markers. In addition, the model expresses relevant brain endothelial transporters and shows spontaneous neuronal firing. The NVU on-a-chip model demonstrates tight barrier function, evidenced by retention of small molecule sodium fluorescein in its lumen. Exposure to the toxic compound staurosporine disrupted the endothelial barrier, causing reduced transepithelial electrical resistance and increased permeability to sodium fluorescein. Under stroke mimicking conditions, brain endothelial cells showed strongly reduced barrier function (35-fold higher apparent permeability) and 7.3-fold decreased mitochondrial potential. Furthermore, levels of adenosine triphosphate were significantly reduced on both the blood- and the brain side of the model (4.8-fold and 11.7-fold reduction, respectively). Conclusions The NVU on-a-chip model presented here can be used for fundamental studies of NVU function in stroke and other neurological diseases and for investigation of potential restorative therapies to fight neurological disorders. Due to the platform’s relatively high throughput and compatibility with automation, the model holds potential for drug compound screening." @default.
• W4200205329 created "2021-12-31" @default.
• W4200205329 creator A5001774953 @default.
• W4200205329 creator A5004415128 @default.
• W4200205329 creator A5018493704 @default.
• W4200205329 creator A5026730500 @default.
• W4200205329 creator A5038811490 @default.
• W4200205329 creator A5040922989 @default.
• W4200205329 creator A5043410411 @default.
• W4200205329 creator A5050148386 @default.
• W4200205329 creator A5055375841 @default.
• W4200205329 creator A5074903993 @default.
• W4200205329 creator A5077272209 @default.
• W4200205329 creator A5077907566 @default.
• W4200205329 date "2021-12-14" @default.
• W4200205329 modified "2023-10-18" @default.
• W4200205329 title "Modeling ischemic stroke in a triculture neurovascular unit on-a-chip" @default.
• W4200205329 cites W1465270826 @default.
• W4200205329 cites W1511717075 @default.
• W4200205329 cites W1554634440 @default.
• W4200205329 cites W1570548198 @default.
• W4200205329 cites W1796054280 @default.
• W4200205329 cites W1847104216 @default.
• W4200205329 cites W1907048502 @default.
• W4200205329 cites W1929386789 @default.
• W4200205329 cites W1964943361 @default.
• W4200205329 cites W1965104493 @default.
• W4200205329 cites W1967081185 @default.
• W4200205329 cites W1968150858 @default.
• W4200205329 cites W1973648711 @default.
• W4200205329 cites W1978208782 @default.
• W4200205329 cites W1980288403 @default.
• W4200205329 cites W1986246946 @default.
• W4200205329 cites W1990253071 @default.
• W4200205329 cites W1991805788 @default.
• W4200205329 cites W1993191156 @default.
• W4200205329 cites W1994504773 @default.
• W4200205329 cites W1994812037 @default.
• W4200205329 cites W1995699866 @default.
• W4200205329 cites W1996719014 @default.
• W4200205329 cites W1996802760 @default.
• W4200205329 cites W1998449043 @default.
• W4200205329 cites W2015541518 @default.
• W4200205329 cites W2016927879 @default.
• W4200205329 cites W2017505808 @default.
• W4200205329 cites W2017844996 @default.
• W4200205329 cites W2019206211 @default.
• W4200205329 cites W2024170393 @default.
• W4200205329 cites W2040536713 @default.
• W4200205329 cites W2041848964 @default.
• W4200205329 cites W2042141282 @default.
• W4200205329 cites W2045312970 @default.
• W4200205329 cites W2045683532 @default.
• W4200205329 cites W2045988433 @default.
• W4200205329 cites W2048975741 @default.
• W4200205329 cites W2055137108 @default.
• W4200205329 cites W2064139713 @default.
• W4200205329 cites W2065083920 @default.
• W4200205329 cites W2069821327 @default.
• W4200205329 cites W2074073922 @default.
• W4200205329 cites W2076104235 @default.
• W4200205329 cites W2081135553 @default.
• W4200205329 cites W2081538957 @default.
• W4200205329 cites W2085146964 @default.
• W4200205329 cites W2088362183 @default.
• W4200205329 cites W2091680093 @default.
• W4200205329 cites W2093744948 @default.
• W4200205329 cites W2104290477 @default.
• W4200205329 cites W2114570899 @default.
• W4200205329 cites W2115533377 @default.
• W4200205329 cites W2122196184 @default.
• W4200205329 cites W2133202642 @default.
• W4200205329 cites W2140405965 @default.
• W4200205329 cites W2144918294 @default.
• W4200205329 cites W2145376030 @default.
• W4200205329 cites W2145613240 @default.
• W4200205329 cites W2154555351 @default.
• W4200205329 cites W2158607478 @default.
• W4200205329 cites W2160213908 @default.
• W4200205329 cites W2162281583 @default.
• W4200205329 cites W2162611158 @default.
• W4200205329 cites W2167279371 @default.
• W4200205329 cites W2167739272 @default.
• W4200205329 cites W2173629634 @default.
• W4200205329 cites W2189120127 @default.
• W4200205329 cites W2260748729 @default.
• W4200205329 cites W2289500363 @default.
• W4200205329 cites W2292308933 @default.
• W4200205329 cites W2294734462 @default.
• W4200205329 cites W2345762450 @default.
• W4200205329 cites W2371948111 @default.
• W4200205329 cites W2400385996 @default.
• W4200205329 cites W2464934969 @default.
• W4200205329 cites W2511446702 @default.
• W4200205329 cites W2553899195 @default.
• W4200205329 cites W2559908181 @default.
• W4200205329 cites W2560319914 @default.
• W4200205329 cites W2560594102 @default.
• W4200205329 cites W2574428496 @default.
• W4200205329 cites W2606185803 @default.

• next