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• W4200211197 abstract "Abstract The frequency of synonymous codons in protein coding genes is non-random and varies both between species and between genes within species. Whether this codon usage bias (CUBias) reflects underlying neutral mutational processes or is instead shaped by selection remains an open debate, especially regarding the role of selection for enhanced protein production. Variation in CUBias of a gene (be it natural synonymous mutations or biotechnological synonymous recoding) can have an enormous impact on its expression by diverse cis -acting mechanisms. But expression of genes with extreme CUBias can also lead to strong phenotypic effects by altering the overall intracellular translation homeostasis via competition for ribosomal machinery or tRNA depletion. In this study, we expressed at high levels in human cells six different synonymous versions of a gene and used matched transcriptomic and proteomic data to evaluate the impact of CUBias of the heterologous gene on the translation of cellular transcripts. Our experimental design focused specifically on differences during translation elongation. Response to expression of the different synonymous sequences was assessed by various approaches, ranging from analyses performed on a per-gene basis to more integrated approaches of the cell as a whole. We observe that the transcriptome displayed substantial changes as a result of heterologous gene expression by triggering an intense antiviral and inflammatory response, but that changes in the proteomes were very modest. Most importantly we notice that changes in translation efficiency of cellular transcripts were not associated with the direction of the CUBias of the heterologous sequences, thereby providing only limited support for trans -acting effects of synonymous changes. We interpret that, in human cells in culture, changes in CUBias can lead to important cis -acting effects in gene expression, but that cellular homeostasis can buffer the phenotypic impact of overexpression of heterologous genes with extreme CUBias." @default.
• W4200211197 created "2021-12-31" @default.
• W4200211197 creator A5002538745 @default.
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• W4200211197 creator A5087959365 @default.
• W4200211197 date "2021-12-10" @default.
• W4200211197 modified "2023-10-18" @default.
• W4200211197 title "Human cellular homeostasis buffers <i>trans</i>-acting translational effects of heterologous gene expression with very different codon usage bias" @default.
• W4200211197 cites W1539868719 @default.
• W4200211197 cites W1550695177 @default.
• W4200211197 cites W1585169926 @default.
• W4200211197 cites W1946157893 @default.
• W4200211197 cites W1956613997 @default.
• W4200211197 cites W1966778471 @default.
• W4200211197 cites W1967656821 @default.
• W4200211197 cites W1968164444 @default.
• W4200211197 cites W1970707135 @default.
• W4200211197 cites W1973268693 @default.
• W4200211197 cites W1974394989 @default.
• W4200211197 cites W1974786493 @default.
• W4200211197 cites W1978527132 @default.
• W4200211197 cites W1991366673 @default.
• W4200211197 cites W1993832002 @default.
• W4200211197 cites W1996769980 @default.
• W4200211197 cites W2004674004 @default.
• W4200211197 cites W2011503994 @default.
• W4200211197 cites W2011828065 @default.
• W4200211197 cites W2012324563 @default.
• W4200211197 cites W2021035007 @default.
• W4200211197 cites W2024800238 @default.
• W4200211197 cites W2034108516 @default.
• W4200211197 cites W2036321220 @default.
• W4200211197 cites W2036897871 @default.
• W4200211197 cites W2039790548 @default.
• W4200211197 cites W2044635447 @default.
• W4200211197 cites W2051242197 @default.
• W4200211197 cites W2055018340 @default.
• W4200211197 cites W2056710301 @default.
• W4200211197 cites W2060421156 @default.
• W4200211197 cites W2061659241 @default.
• W4200211197 cites W2063779182 @default.
• W4200211197 cites W2065120930 @default.
• W4200211197 cites W2066218700 @default.
• W4200211197 cites W2066829062 @default.
• W4200211197 cites W2070163065 @default.
• W4200211197 cites W2073010081 @default.
• W4200211197 cites W2075158468 @default.
• W4200211197 cites W2076793571 @default.
• W4200211197 cites W2078146553 @default.
• W4200211197 cites W2078755055 @default.
• W4200211197 cites W2079228567 @default.
• W4200211197 cites W2080752012 @default.
• W4200211197 cites W2087439897 @default.
• W4200211197 cites W2095868955 @default.
• W4200211197 cites W2122683221 @default.
• W4200211197 cites W2124969332 @default.
• W4200211197 cites W2128551987 @default.
• W4200211197 cites W2131271579 @default.
• W4200211197 cites W2132117253 @default.
• W4200211197 cites W2134541788 @default.
• W4200211197 cites W2141472216 @default.
• W4200211197 cites W2141751671 @default.
• W4200211197 cites W2144652247 @default.
• W4200211197 cites W2148734328 @default.
• W4200211197 cites W2151178734 @default.
• W4200211197 cites W2151632575 @default.
• W4200211197 cites W2159365979 @default.
• W4200211197 cites W2160514369 @default.
• W4200211197 cites W2162598202 @default.
• W4200211197 cites W2167725902 @default.
• W4200211197 cites W2170513170 @default.
• W4200211197 cites W2170776626 @default.
• W4200211197 cites W2171897290 @default.
• W4200211197 cites W2259283565 @default.
• W4200211197 cites W2323326409 @default.
• W4200211197 cites W2463195069 @default.
• W4200211197 cites W2568577966 @default.
• W4200211197 cites W2793056129 @default.
• W4200211197 cites W2803275252 @default.
• W4200211197 cites W2892180666 @default.
• W4200211197 cites W2949566896 @default.
• W4200211197 cites W2950512597 @default.
• W4200211197 cites W2950681580 @default.
• W4200211197 cites W2962101943 @default.
• W4200211197 cites W2962480734 @default.
• W4200211197 cites W2965875787 @default.
• W4200211197 cites W2983889514 @default.
• W4200211197 cites W2993396897 @default.
• W4200211197 cites W3004726194 @default.
• W4200211197 cites W3095839623 @default.
• W4200211197 cites W3101518221 @default.
• W4200211197 cites W3126835343 @default.
• W4200211197 cites W3136308181 @default.
• W4200211197 doi "https://doi.org/10.1101/2021.12.09.471957" @default.
• W4200211197 hasPublicationYear "2021" @default.
• W4200211197 type Work @default.

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