Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200214531> ?p ?o ?g. }
- W4200214531 abstract "Cryo-electron microscopy (cryo-EM) has become a powerful tool to resolve high-resolution structures of biomacromolecules in solution. However, air-water interface induced preferred orientations, dissociation or denaturation of biomacromolecules during cryo-vitrification remains a limiting factor for many specimens. To solve this bottleneck, we developed a cryo-EM support film using 2D crystals of hydrophobin HFBI. The hydrophilic side of the HFBI film adsorbs protein particles via electrostatic interactions and sequesters them from the air-water interface, allowing the formation of sufficiently thin ice for high-quality data collection. The particle orientation distribution can be regulated by adjusting the buffer pH. Using this support, we determined the cryo-EM structures of catalase (2.29 Å) and influenza haemagglutinin trimer (2.56 Å), which exhibited strong preferred orientations using a conventional cryo-vitrification protocol. We further show that the HFBI film is suitable to obtain high-resolution structures of small proteins, including aldolase (150 kDa, 3.28 Å) and haemoglobin (64 kDa, 3.6 Å). Our work suggests that HFBI films may have broad future applications in increasing the success rate and efficiency of cryo-EM." @default.
- W4200214531 created "2021-12-31" @default.
- W4200214531 creator A5011309001 @default.
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- W4200214531 creator A5089028342 @default.
- W4200214531 creator A5090316903 @default.
- W4200214531 date "2021-12-14" @default.
- W4200214531 modified "2023-10-05" @default.
- W4200214531 title "A cryo-electron microscopy support film formed by 2D crystals of hydrophobin HFBI" @default.
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- W4200214531 doi "https://doi.org/10.1038/s41467-021-27596-8" @default.