Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200220105> ?p ?o ?g. }
- W4200220105 abstract "Macrophages play important roles in angiogenesis; however, previous studies on macrophage angiogenesis have focused on traditional 2D cultures. In this study, we established a 3D culture system for macrophages using collagen microcarriers and assessed the effect of 3D culture on their angiogenic capabilities. Macrophages grown in 3D culture displayed a significantly different morphology and arrangement under electron microscopy compared to those grown in 2D culture. Tube formation assays and chick embryo chorioallantoic membrane assays further revealed that 3D-cultured macrophages were less angiogenic than those in 2D culture. Whole-transcriptome sequencing showed that nearly 40% of genes were significantly differently expressed, including nine important angiogenic factors of which seven had been downregulated. In addition, the expression of almost all genes related to two important angiogenic pathways was decreased in 3D-cultured macrophages, including the two key angiogenic factors, VEGFA and ANG2. Together, the findings of our study improve our understanding of angiogenesis and 3D macrophage culture in tissues, and provide new avenues and methods for future research on macrophages." @default.
- W4200220105 created "2021-12-31" @default.
- W4200220105 creator A5000632526 @default.
- W4200220105 creator A5008602430 @default.
- W4200220105 creator A5008902636 @default.
- W4200220105 creator A5017607299 @default.
- W4200220105 creator A5019589675 @default.
- W4200220105 creator A5042601890 @default.
- W4200220105 creator A5051525695 @default.
- W4200220105 creator A5056036725 @default.
- W4200220105 date "2021-12-22" @default.
- W4200220105 modified "2023-10-02" @default.
- W4200220105 title "Three-Dimensional Culture Decreases the Angiogenic Ability of Mouse Macrophages" @default.
- W4200220105 cites W107561333 @default.
- W4200220105 cites W1965975485 @default.
- W4200220105 cites W1978018743 @default.
- W4200220105 cites W1985043086 @default.
- W4200220105 cites W1992814996 @default.
- W4200220105 cites W2017335731 @default.
- W4200220105 cites W2038448543 @default.
- W4200220105 cites W2047266588 @default.
- W4200220105 cites W2053221827 @default.
- W4200220105 cites W2055348159 @default.
- W4200220105 cites W2065210647 @default.
- W4200220105 cites W2077129920 @default.
- W4200220105 cites W2126811287 @default.
- W4200220105 cites W2130356372 @default.
- W4200220105 cites W2132981126 @default.
- W4200220105 cites W2146560578 @default.
- W4200220105 cites W2156324629 @default.
- W4200220105 cites W2172253185 @default.
- W4200220105 cites W2253213520 @default.
- W4200220105 cites W2331628193 @default.
- W4200220105 cites W2479858070 @default.
- W4200220105 cites W2511728841 @default.
- W4200220105 cites W2763341318 @default.
- W4200220105 cites W2766037597 @default.
- W4200220105 cites W2794101039 @default.
- W4200220105 cites W2795744904 @default.
- W4200220105 cites W2799997987 @default.
- W4200220105 cites W2806159238 @default.
- W4200220105 cites W2884241852 @default.
- W4200220105 cites W2887086124 @default.
- W4200220105 cites W2894988139 @default.
- W4200220105 cites W2982653531 @default.
- W4200220105 cites W3022902771 @default.
- W4200220105 cites W3033137286 @default.
- W4200220105 cites W3186088909 @default.
- W4200220105 cites W972043883 @default.
- W4200220105 doi "https://doi.org/10.3389/fimmu.2021.795066" @default.
- W4200220105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35003117" @default.
- W4200220105 hasPublicationYear "2021" @default.
- W4200220105 type Work @default.
- W4200220105 citedByCount "0" @default.
- W4200220105 crossrefType "journal-article" @default.
- W4200220105 hasAuthorship W4200220105A5000632526 @default.
- W4200220105 hasAuthorship W4200220105A5008602430 @default.
- W4200220105 hasAuthorship W4200220105A5008902636 @default.
- W4200220105 hasAuthorship W4200220105A5017607299 @default.
- W4200220105 hasAuthorship W4200220105A5019589675 @default.
- W4200220105 hasAuthorship W4200220105A5042601890 @default.
- W4200220105 hasAuthorship W4200220105A5051525695 @default.
- W4200220105 hasAuthorship W4200220105A5056036725 @default.
- W4200220105 hasBestOaLocation W42002201051 @default.
- W4200220105 hasConcept C104317684 @default.
- W4200220105 hasConcept C150194340 @default.
- W4200220105 hasConcept C162317418 @default.
- W4200220105 hasConcept C176999471 @default.
- W4200220105 hasConcept C196843134 @default.
- W4200220105 hasConcept C202751555 @default.
- W4200220105 hasConcept C203014093 @default.
- W4200220105 hasConcept C2778271429 @default.
- W4200220105 hasConcept C2779244956 @default.
- W4200220105 hasConcept C2780394083 @default.
- W4200220105 hasConcept C502942594 @default.
- W4200220105 hasConcept C54355233 @default.
- W4200220105 hasConcept C81885089 @default.
- W4200220105 hasConcept C86803240 @default.
- W4200220105 hasConcept C95444343 @default.
- W4200220105 hasConcept C97230052 @default.
- W4200220105 hasConceptScore W4200220105C104317684 @default.
- W4200220105 hasConceptScore W4200220105C150194340 @default.
- W4200220105 hasConceptScore W4200220105C162317418 @default.
- W4200220105 hasConceptScore W4200220105C176999471 @default.
- W4200220105 hasConceptScore W4200220105C196843134 @default.
- W4200220105 hasConceptScore W4200220105C202751555 @default.
- W4200220105 hasConceptScore W4200220105C203014093 @default.
- W4200220105 hasConceptScore W4200220105C2778271429 @default.
- W4200220105 hasConceptScore W4200220105C2779244956 @default.
- W4200220105 hasConceptScore W4200220105C2780394083 @default.
- W4200220105 hasConceptScore W4200220105C502942594 @default.
- W4200220105 hasConceptScore W4200220105C54355233 @default.
- W4200220105 hasConceptScore W4200220105C81885089 @default.
- W4200220105 hasConceptScore W4200220105C86803240 @default.
- W4200220105 hasConceptScore W4200220105C95444343 @default.
- W4200220105 hasConceptScore W4200220105C97230052 @default.
- W4200220105 hasLocation W42002201051 @default.
- W4200220105 hasLocation W42002201052 @default.
- W4200220105 hasLocation W42002201053 @default.
- W4200220105 hasLocation W42002201054 @default.