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• W4200220160 endingPage "1055" @default.
• W4200220160 startingPage "1055" @default.
• W4200220160 abstract "Dimethyl sulfoxide (DSMO) is a simple molecule widely used because of its great solvating ability, but this solvent also has little-known biological effects, especially on fungi. Aspergillus flavus is a notorious pathogenic fungus which may contaminate a large variety of crops worldwide by producing aflatoxins, endangering at the same time food safety and international trade. The aim of this study was to characterize the effect of DMSO on A. flavus including developmental parameters such as germination and sporulation, as well as its transcriptome profile using high-throughput RNA-sequencing assay and its impact on secondary metabolism (SM). After DMSO exposure, A. flavus displayed depigmented conidia in a dose-dependent manner. The four-day exposition of cultures to two doses of DMSO, chosen on the basis of depigmentation intensity (35 mM low and 282 mM high), led to no significant impact on fungal growth, germination or sporulation. However, transcriptomic data analysis showed that 4891 genes were differentially regulated in response to DMSO (46% of studied transcripts). A total of 4650 genes were specifically regulated in response to the highest dose of DMSO, while only 19 genes were modulated upon exposure to the lowest dose. Secondary metabolites clusters genes were widely affected by the DMSO, with 91% of clusters impacted at the highest dose. Among these, aflatoxins, cyclopiazonic acid and ustiloxin B clusters were totally under-expressed. The genes belonging to the AFB1 cluster were the most negatively modulated ones, the two doses leading to 63% and 100% inhibition of the AFB1 production, respectively. The SM analysis also showed the disappearance of ustiloxin B and a 10-fold reduction of cyclopiazonic acid level when A. flavus was treated by the higher DMSO dose. In conclusion, the present study showed that DMSO impacted widely A. flavus' transcriptome, including secondary metabolism gene clusters with the aflatoxins at the head of down-regulated ones. The solvent also inhibits conidial pigmentation, which could illustrate common regulatory mechanisms between aflatoxins and fungal pigment pathways. Because of its effect on major metabolites synthesis, DMSO should not be used as solvent especially in studies testing anti-aflatoxinogenic compounds." @default.
• W4200220160 created "2021-12-31" @default.
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• W4200220160 date "2021-12-09" @default.
• W4200220160 modified "2023-10-05" @default.
• W4200220160 title "The Solvent Dimethyl Sulfoxide Affects Physiology, Transcriptome and Secondary Metabolism of Aspergillus flavus" @default.
• W4200220160 cites W1757066578 @default.
• W4200220160 cites W1837548183 @default.
• W4200220160 cites W1845631646 @default.
• W4200220160 cites W1969353942 @default.
• W4200220160 cites W1971309707 @default.
• W4200220160 cites W1971325910 @default.
• W4200220160 cites W1977878747 @default.
• W4200220160 cites W1981204101 @default.
• W4200220160 cites W1991276321 @default.
• W4200220160 cites W1992682143 @default.
• W4200220160 cites W1998263994 @default.
• W4200220160 cites W2005758645 @default.
• W4200220160 cites W2006032735 @default.
• W4200220160 cites W2011130109 @default.
• W4200220160 cites W2014341995 @default.
• W4200220160 cites W2018652426 @default.
• W4200220160 cites W2020541351 @default.
• W4200220160 cites W2024368780 @default.
• W4200220160 cites W2029283335 @default.
• W4200220160 cites W2031539920 @default.
• W4200220160 cites W2031904808 @default.
• W4200220160 cites W2033189806 @default.
• W4200220160 cites W2034951809 @default.
• W4200220160 cites W2036718342 @default.
• W4200220160 cites W2050785055 @default.
• W4200220160 cites W2052375196 @default.
• W4200220160 cites W2053098819 @default.
• W4200220160 cites W2055825617 @default.
• W4200220160 cites W2058125499 @default.
• W4200220160 cites W2060203705 @default.
• W4200220160 cites W2062782211 @default.
• W4200220160 cites W2063587479 @default.
• W4200220160 cites W2066799690 @default.
• W4200220160 cites W2067379020 @default.
• W4200220160 cites W2078788423 @default.
• W4200220160 cites W2078920173 @default.
• W4200220160 cites W2080346786 @default.
• W4200220160 cites W2083237836 @default.
• W4200220160 cites W2086801210 @default.
• W4200220160 cites W2088150699 @default.
• W4200220160 cites W2094245023 @default.
• W4200220160 cites W2095254105 @default.
• W4200220160 cites W2097624556 @default.
• W4200220160 cites W2098664588 @default.
• W4200220160 cites W2100661439 @default.
• W4200220160 cites W2103163042 @default.
• W4200220160 cites W2110065044 @default.
• W4200220160 cites W2114104545 @default.
• W4200220160 cites W2120013600 @default.
• W4200220160 cites W2120117970 @default.
• W4200220160 cites W2138207763 @default.
• W4200220160 cites W2146918829 @default.
• W4200220160 cites W2151596329 @default.
• W4200220160 cites W2155072854 @default.
• W4200220160 cites W2169256073 @default.
• W4200220160 cites W2201339123 @default.
• W4200220160 cites W2267312515 @default.
• W4200220160 cites W2344046325 @default.
• W4200220160 cites W2465897665 @default.
• W4200220160 cites W2532882443 @default.
• W4200220160 cites W2539809107 @default.
• W4200220160 cites W2594265056 @default.
• W4200220160 cites W2606936587 @default.
• W4200220160 cites W2618918038 @default.
• W4200220160 cites W2750291400 @default.
• W4200220160 cites W2754161078 @default.
• W4200220160 cites W2802646500 @default.
• W4200220160 cites W2811395485 @default.
• W4200220160 cites W2888160344 @default.
• W4200220160 cites W3010056058 @default.
• W4200220160 cites W3039680345 @default.
• W4200220160 cites W3094576192 @default.
• W4200220160 cites W3126842349 @default.
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• W4200220160 doi "https://doi.org/10.3390/jof7121055" @default.
• W4200220160 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34947037" @default.
• W4200220160 hasPublicationYear "2021" @default.
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