FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200264831> ?p ?o ?g. }

• W4200264831 abstract "Hepatitis C virus (HCV) genotype 3 is widely distributed, and genotype 3-infected patients achieve a lower cure rate in direct-acting antiviral (DAA) therapy and are associated with a higher risk of hepatic steatosis than patients with other genotypes. Thus, the study of the virology and pathogenesis of genotype 3 HCV is increasingly relevant. Here, we developed a full-length infectious clone and a subgenomic replicon for the genotype 3a isolate, CH3a. From an infected serum, we constructed a full-length CH3a clone, however, it was nonviable in Huh7.5.1 cells. Next, we systematically adapted several intergenotypic recombinants containing Core-NS2 and 5'UTR-NS5A from CH3a, and other sequences from a replication-competent genotype 2 a clone JFH1. Adaptive mutations were identified, of which several combinations facilitated the replication of CH3a-JFH1 recombinants; however, they failed to adapt to the full-length CH3a and the recombinants containing CH3a NS5B. Thus, we attempted to separately adapt CH3a NS5B-3'UTR by constructing an intragenotypic recombinant using 5'UTR-NS5A from an infectious genotype 3a clone, DBN3acc, from which L3004P/M in NS5B and a deletion of 11 nucleotides (Δ11nt) downstream of the polyU/UC tract of the 3'UTR were identified and demonstrated to efficiently improve virus production. Finally, we combined functional 5'UTR-NS5A and NS5B-3'UTR sequences that carried the selected mutations to generate full-length CH3a with 26 or 27 substitutions (CH3acc), and both revealed efficient replication and virus spread in transfected and infected cells, releasing HCV of 104.2 f.f.u. ml-1. CH3acc was inhibited by DAAs targeting NS3/4A, NS5A and NS5B in a dose-dependent manner. The selected mutations permitted the development of subgenomic replicon CH3a-SGRep, by which L3004P, L3004M and Δ11nt were proven, together with a single-cycle virus production assay, to facilitate virus assembly, release, and RNA replication. CH3acc clones and CH3a-SGRep replicon provide new tools for the study of HCV genotype 3." @default.
• W4200264831 created "2021-12-31" @default.
• W4200264831 creator A5000939253 @default.
• W4200264831 creator A5015986011 @default.
• W4200264831 creator A5019237114 @default.
• W4200264831 creator A5023250695 @default.
• W4200264831 creator A5036978179 @default.
• W4200264831 creator A5042384343 @default.
• W4200264831 creator A5043356374 @default.
• W4200264831 creator A5050720879 @default.
• W4200264831 creator A5055276286 @default.
• W4200264831 creator A5067069882 @default.
• W4200264831 creator A5088076863 @default.
• W4200264831 date "2021-12-24" @default.
• W4200264831 modified "2023-10-16" @default.
• W4200264831 title "Development of full-length cell-culture infectious clone and subgenomic replicon for a genotype 3a isolate of hepatitis C virus" @default.
• W4200264831 cites W1512078611 @default.
• W4200264831 cites W1792056958 @default.
• W4200264831 cites W1978130780 @default.
• W4200264831 cites W1980805512 @default.
• W4200264831 cites W1987263725 @default.
• W4200264831 cites W1994123538 @default.
• W4200264831 cites W1997005774 @default.
• W4200264831 cites W1998002462 @default.
• W4200264831 cites W2011893864 @default.
• W4200264831 cites W2028831570 @default.
• W4200264831 cites W2034241642 @default.
• W4200264831 cites W2044193462 @default.
• W4200264831 cites W2083200314 @default.
• W4200264831 cites W2096347191 @default.
• W4200264831 cites W2109733403 @default.
• W4200264831 cites W2115917312 @default.
• W4200264831 cites W2121209352 @default.
• W4200264831 cites W2131143399 @default.
• W4200264831 cites W2132034333 @default.
• W4200264831 cites W2133036275 @default.
• W4200264831 cites W2138488044 @default.
• W4200264831 cites W2146277683 @default.
• W4200264831 cites W2157733066 @default.
• W4200264831 cites W2159094299 @default.
• W4200264831 cites W2160788656 @default.
• W4200264831 cites W2166549128 @default.
• W4200264831 cites W2284365217 @default.
• W4200264831 cites W2394500959 @default.
• W4200264831 cites W2495212599 @default.
• W4200264831 cites W2555314306 @default.
• W4200264831 cites W2560002598 @default.
• W4200264831 cites W2589788641 @default.
• W4200264831 cites W2768722273 @default.
• W4200264831 cites W2792290848 @default.
• W4200264831 cites W2809785330 @default.
• W4200264831 cites W2899780877 @default.
• W4200264831 cites W2902583913 @default.
• W4200264831 cites W2991889508 @default.
• W4200264831 cites W2998988013 @default.
• W4200264831 cites W3088020815 @default.
• W4200264831 cites W3092401676 @default.
• W4200264831 cites W3131386574 @default.
• W4200264831 cites W3157589800 @default.
• W4200264831 cites W4211014289 @default.
• W4200264831 doi "https://doi.org/10.1099/jgv.0.001704" @default.
• W4200264831 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34949310" @default.
• W4200264831 hasPublicationYear "2021" @default.
• W4200264831 type Work @default.
• W4200264831 citedByCount "1" @default.
• W4200264831 countsByYear W42002648312022 @default.
• W4200264831 crossrefType "journal-article" @default.
• W4200264831 hasAuthorship W4200264831A5000939253 @default.
• W4200264831 hasAuthorship W4200264831A5015986011 @default.
• W4200264831 hasAuthorship W4200264831A5019237114 @default.
• W4200264831 hasAuthorship W4200264831A5023250695 @default.
• W4200264831 hasAuthorship W4200264831A5036978179 @default.
• W4200264831 hasAuthorship W4200264831A5042384343 @default.
• W4200264831 hasAuthorship W4200264831A5043356374 @default.
• W4200264831 hasAuthorship W4200264831A5050720879 @default.
• W4200264831 hasAuthorship W4200264831A5055276286 @default.
• W4200264831 hasAuthorship W4200264831A5067069882 @default.
• W4200264831 hasAuthorship W4200264831A5088076863 @default.
• W4200264831 hasConcept C104317684 @default.
• W4200264831 hasConcept C135763542 @default.
• W4200264831 hasConcept C140704245 @default.
• W4200264831 hasConcept C159047783 @default.
• W4200264831 hasConcept C18103114 @default.
• W4200264831 hasConcept C189819185 @default.
• W4200264831 hasConcept C22744801 @default.
• W4200264831 hasConcept C2522874641 @default.
• W4200264831 hasConcept C2776408679 @default.
• W4200264831 hasConcept C2777103181 @default.
• W4200264831 hasConcept C2781426373 @default.
• W4200264831 hasConcept C2781463415 @default.
• W4200264831 hasConcept C54355233 @default.
• W4200264831 hasConcept C67705224 @default.
• W4200264831 hasConcept C81089528 @default.
• W4200264831 hasConcept C86803240 @default.
• W4200264831 hasConcept C89604277 @default.
• W4200264831 hasConcept C98108389 @default.
• W4200264831 hasConceptScore W4200264831C104317684 @default.
• W4200264831 hasConceptScore W4200264831C135763542 @default.
• W4200264831 hasConceptScore W4200264831C140704245 @default.
• W4200264831 hasConceptScore W4200264831C159047783 @default.

• next