FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200265100> ?p ?o ?g. }

• W4200265100 endingPage "101422" @default.
• W4200265100 startingPage "101422" @default.
• W4200265100 abstract "Growth differentiation factor 15 (GDF15) is known to play a role in feeding, nausea, and body weight, with action through the GFRAL-RET receptor complex in the area postrema (AP) and nucleus tractus solitarius (NTS). To further elucidate the underlying cell type-specific molecular mechanisms downstream of GDF15 signaling, we used a single nuclei RNA sequencing (snRNAseq) approach to profile AP and NTS cellular subtype-specific transcriptomes after systemic GDF15 treatment.AP and NTS micropunches were used for snRNAseq from Sprague Dawley rats 6 h following GDF15 or saline injection, and Seurat was used to identify cellular subtypes and cell type-specific alterations in gene expression that were due to the direct and secondary effects of systemic GDF15 treatment.Using the transcriptome profile of ∼35,000 individual AP/NTS nuclei, we identified 19 transcriptomically distinct cellular subtypes, including a single population Gfral and Ret positive excitatory neurons, representing the primary site of action for GDF15. A total of ∼600 cell type-specific differential expression events were identified in neurons and glia, including the identification of transcriptome alterations specific to the direct effects of GDF15 in the Gfral-Ret positive excitatory neurons and shared transcriptome alterations across neuronal and glial cell types. Downstream analyses identified shared and cell type-specific alterations in signaling pathways and upstream regulatory mechanisms of the observed transcriptome alterations.These data provide a considerable advance in our understanding of AP and NTS cell type-specific molecular mechanisms associated with GDF15 signaling. The identified cellular subtype-specific regulatory mechanism and signaling pathways likely represent important targets for future pharmacotherapies." @default.
• W4200265100 created "2021-12-31" @default.
• W4200265100 creator A5006965852 @default.
• W4200265100 creator A5010432339 @default.
• W4200265100 creator A5027913658 @default.
• W4200265100 creator A5057917339 @default.
• W4200265100 creator A5065258001 @default.
• W4200265100 creator A5079833063 @default.
• W4200265100 date "2022-02-01" @default.
• W4200265100 modified "2023-10-16" @default.
• W4200265100 title "Single nuclei RNA sequencing of the rat AP and NTS following GDF15 treatment" @default.
• W4200265100 cites W1507364611 @default.
• W4200265100 cites W1974379520 @default.
• W4200265100 cites W1975913194 @default.
• W4200265100 cites W1993434718 @default.
• W4200265100 cites W2010457001 @default.
• W4200265100 cites W2012779561 @default.
• W4200265100 cites W2020474446 @default.
• W4200265100 cites W2066775550 @default.
• W4200265100 cites W2072276134 @default.
• W4200265100 cites W2078910663 @default.
• W4200265100 cites W2082583113 @default.
• W4200265100 cites W2088864173 @default.
• W4200265100 cites W2098813173 @default.
• W4200265100 cites W2117304506 @default.
• W4200265100 cites W2126336330 @default.
• W4200265100 cites W2162143298 @default.
• W4200265100 cites W2166643977 @default.
• W4200265100 cites W2168430622 @default.
• W4200265100 cites W2314466696 @default.
• W4200265100 cites W2330887677 @default.
• W4200265100 cites W2333386541 @default.
• W4200265100 cites W2746853643 @default.
• W4200265100 cites W2748135921 @default.
• W4200265100 cites W2750180661 @default.
• W4200265100 cites W2759628183 @default.
• W4200265100 cites W2767260867 @default.
• W4200265100 cites W2805288114 @default.
• W4200265100 cites W2891822635 @default.
• W4200265100 cites W2897503007 @default.
• W4200265100 cites W2911867504 @default.
• W4200265100 cites W2942678593 @default.
• W4200265100 cites W2946016273 @default.
• W4200265100 cites W2951560273 @default.
• W4200265100 cites W3000046815 @default.
• W4200265100 cites W3017224205 @default.
• W4200265100 cites W3020538763 @default.
• W4200265100 cites W3045596688 @default.
• W4200265100 cites W3096036094 @default.
• W4200265100 cites W3106772924 @default.
• W4200265100 cites W3137550402 @default.
• W4200265100 cites W3154638647 @default.
• W4200265100 cites W3158537114 @default.
• W4200265100 cites W3175122357 @default.
• W4200265100 cites W3187492301 @default.
• W4200265100 cites W3192768025 @default.
• W4200265100 cites W3196868198 @default.
• W4200265100 cites W3197424737 @default.
• W4200265100 doi "https://doi.org/10.1016/j.molmet.2021.101422" @default.
• W4200265100 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34942400" @default.
• W4200265100 hasPublicationYear "2022" @default.
• W4200265100 type Work @default.
• W4200265100 citedByCount "6" @default.
• W4200265100 countsByYear W42002651002022 @default.
• W4200265100 countsByYear W42002651002023 @default.
• W4200265100 crossrefType "journal-article" @default.
• W4200265100 hasAuthorship W4200265100A5006965852 @default.
• W4200265100 hasAuthorship W4200265100A5010432339 @default.
• W4200265100 hasAuthorship W4200265100A5027913658 @default.
• W4200265100 hasAuthorship W4200265100A5057917339 @default.
• W4200265100 hasAuthorship W4200265100A5065258001 @default.
• W4200265100 hasAuthorship W4200265100A5079833063 @default.
• W4200265100 hasBestOaLocation W42002651003 @default.
• W4200265100 hasConcept C104317684 @default.
• W4200265100 hasConcept C136935669 @default.
• W4200265100 hasConcept C1491633281 @default.
• W4200265100 hasConcept C150194340 @default.
• W4200265100 hasConcept C162317418 @default.
• W4200265100 hasConcept C189014844 @default.
• W4200265100 hasConcept C2908647359 @default.
• W4200265100 hasConcept C54355233 @default.
• W4200265100 hasConcept C62478195 @default.
• W4200265100 hasConcept C71924100 @default.
• W4200265100 hasConcept C86803240 @default.
• W4200265100 hasConcept C95444343 @default.
• W4200265100 hasConcept C99454951 @default.
• W4200265100 hasConceptScore W4200265100C104317684 @default.
• W4200265100 hasConceptScore W4200265100C136935669 @default.
• W4200265100 hasConceptScore W4200265100C1491633281 @default.
• W4200265100 hasConceptScore W4200265100C150194340 @default.
• W4200265100 hasConceptScore W4200265100C162317418 @default.
• W4200265100 hasConceptScore W4200265100C189014844 @default.
• W4200265100 hasConceptScore W4200265100C2908647359 @default.
• W4200265100 hasConceptScore W4200265100C54355233 @default.
• W4200265100 hasConceptScore W4200265100C62478195 @default.
• W4200265100 hasConceptScore W4200265100C71924100 @default.
• W4200265100 hasConceptScore W4200265100C86803240 @default.
• W4200265100 hasConceptScore W4200265100C95444343 @default.

• next