Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200293148> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W4200293148 abstract "Abstract Background: The mechanisms underlying dysfunction of choroid plexus (ChP) blood-cerebrospinal fluid (CSF) barrier and lymphocyte invasion in neuroinflammatory responses to stroke are not well understood. In this study, we investigated whether stroke damaged the blood-CSF barrier integrity due to dysregulation of major ChP ion transport system Na+-K+-Cl- cotransporter (NKCC1) and regulatory Ste20-related proline-alanine-rich kinase (SPAK). Methods: Sham or ischemic stroke was induced in C57Bl/6J mice. Changes of the SPAK-NKCC1 complex and tight junction proteins (TJs) in the ChP were quantified by immunofluorescence staining and immunoblotting. Immune cell infiltration in the ChP was assessed by flow cytometry and immunostaining. Cultured ChP epithelium cells (CPECs) and cortical neurons were used to evaluate H2O2-mediated oxidative stress in stimulating the SPAK-NKCC1 complex and cellular damage. In vivo or in vitro pharmacological blockade of the ChP SPAK-NKCC1 cascade with SPAK inhibitor ZT-1a or NKCC1 inhibitor bumetanide were examined. Results: Ischemic stroke stimulated activation of the CPECs apical membrane SPAK-NKCC1 complex, NF-κB, and MMP9, which was associated with loss of the blood-CSF barrier integrity and increased immune cell infiltration into the ChP. Oxidative stress directly activated SPAK-NKCC1 pathway and resulted in apoptosis, neurodegeneration, and NKCC1-mediated ion influx. Pharmacological blockade of the SPAK-NKCC1 pathway protected the ChP barrier integrity, attenuated ChP immune cell infiltration or neuronal death. Conclusion: Stroke-induced pathological stimulation of the SPAK-NKCC1 cascade caused CPECs damage and disruption of TJs at the blood-CSF barrier. The ChP SPAK-NKCC1 complex emerged as a therapeutic target for attenuating ChP dysfunction and lymphocyte invasion after stroke." @default.
- W4200293148 created "2021-12-31" @default.
- W4200293148 creator A5001364115 @default.
- W4200293148 creator A5013649577 @default.
- W4200293148 creator A5020819058 @default.
- W4200293148 creator A5021389716 @default.
- W4200293148 creator A5021885543 @default.
- W4200293148 creator A5022478300 @default.
- W4200293148 creator A5033378538 @default.
- W4200293148 creator A5042241049 @default.
- W4200293148 creator A5047990812 @default.
- W4200293148 creator A5049938481 @default.
- W4200293148 creator A5050776065 @default.
- W4200293148 creator A5058936141 @default.
- W4200293148 creator A5076671960 @default.
- W4200293148 creator A5079477430 @default.
- W4200293148 creator A5085120246 @default.
- W4200293148 creator A5085545519 @default.
- W4200293148 creator A5086872650 @default.
- W4200293148 date "2021-12-29" @default.
- W4200293148 modified "2023-10-01" @default.
- W4200293148 title "Role of SPAK-NKCC1 Signaling Cascade in the Choroid Plexus Blood-CSF Barrier Damage After Stroke" @default.
- W4200293148 doi "https://doi.org/10.21203/rs.3.rs-1189368/v1" @default.
- W4200293148 hasPublicationYear "2021" @default.
- W4200293148 type Work @default.
- W4200293148 citedByCount "0" @default.
- W4200293148 crossrefType "posted-content" @default.
- W4200293148 hasAuthorship W4200293148A5001364115 @default.
- W4200293148 hasAuthorship W4200293148A5013649577 @default.
- W4200293148 hasAuthorship W4200293148A5020819058 @default.
- W4200293148 hasAuthorship W4200293148A5021389716 @default.
- W4200293148 hasAuthorship W4200293148A5021885543 @default.
- W4200293148 hasAuthorship W4200293148A5022478300 @default.
- W4200293148 hasAuthorship W4200293148A5033378538 @default.
- W4200293148 hasAuthorship W4200293148A5042241049 @default.
- W4200293148 hasAuthorship W4200293148A5047990812 @default.
- W4200293148 hasAuthorship W4200293148A5049938481 @default.
- W4200293148 hasAuthorship W4200293148A5050776065 @default.
- W4200293148 hasAuthorship W4200293148A5058936141 @default.
- W4200293148 hasAuthorship W4200293148A5076671960 @default.
- W4200293148 hasAuthorship W4200293148A5079477430 @default.
- W4200293148 hasAuthorship W4200293148A5085120246 @default.
- W4200293148 hasAuthorship W4200293148A5085545519 @default.
- W4200293148 hasAuthorship W4200293148A5086872650 @default.
- W4200293148 hasBestOaLocation W42002931481 @default.
- W4200293148 hasConcept C134018914 @default.
- W4200293148 hasConcept C185592680 @default.
- W4200293148 hasConcept C2776151105 @default.
- W4200293148 hasConcept C2776538271 @default.
- W4200293148 hasConcept C2778402981 @default.
- W4200293148 hasConcept C529278444 @default.
- W4200293148 hasConcept C55493867 @default.
- W4200293148 hasConcept C86803240 @default.
- W4200293148 hasConcept C95444343 @default.
- W4200293148 hasConcept C98274493 @default.
- W4200293148 hasConceptScore W4200293148C134018914 @default.
- W4200293148 hasConceptScore W4200293148C185592680 @default.
- W4200293148 hasConceptScore W4200293148C2776151105 @default.
- W4200293148 hasConceptScore W4200293148C2776538271 @default.
- W4200293148 hasConceptScore W4200293148C2778402981 @default.
- W4200293148 hasConceptScore W4200293148C529278444 @default.
- W4200293148 hasConceptScore W4200293148C55493867 @default.
- W4200293148 hasConceptScore W4200293148C86803240 @default.
- W4200293148 hasConceptScore W4200293148C95444343 @default.
- W4200293148 hasConceptScore W4200293148C98274493 @default.
- W4200293148 hasLocation W42002931481 @default.
- W4200293148 hasLocation W42002931482 @default.
- W4200293148 hasLocation W42002931483 @default.
- W4200293148 hasOpenAccess W4200293148 @default.
- W4200293148 hasPrimaryLocation W42002931481 @default.
- W4200293148 hasRelatedWork W1454519016 @default.
- W4200293148 hasRelatedWork W1529398063 @default.
- W4200293148 hasRelatedWork W1542251585 @default.
- W4200293148 hasRelatedWork W189350192 @default.
- W4200293148 hasRelatedWork W2071052390 @default.
- W4200293148 hasRelatedWork W2521299414 @default.
- W4200293148 hasRelatedWork W2899084033 @default.
- W4200293148 hasRelatedWork W2905019447 @default.
- W4200293148 hasRelatedWork W3165448279 @default.
- W4200293148 hasRelatedWork W7818955 @default.
- W4200293148 isParatext "false" @default.
- W4200293148 isRetracted "false" @default.
- W4200293148 workType "article" @default.