Matches in SemOpenAlex for { <https://semopenalex.org/work/W4200295615> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W4200295615 abstract "This manuscript describes a protocol for detecting transcription termination defect in vivo. The strand-specific TRO protocol using BrUTP described here is a powerful experimental approach for analyzing the transcription termination defect under physiological conditions. Like the traditional TRO assay, it relies on the presence of a transcriptionally active polymerase beyond the 3′ end of the gene as an indicator of a transcription termination defect1. It overcomes two major problems encountered with the traditional TRO assay. First, it can detect if the polymerase reading through the termination signal is the one that initiated transcription from the promoter-proximal region, or if it is simply representing a pervasively transcribing polymerase that initiated non-specifically from somewhere in the body or the 3′ end of the gene. Secondly, it can distinguish if the transcriptionally active polymerase signal beyond the terminator region is truly the readthrough sense mRNA transcribing polymerase or a terminator-initiated non-coding anti-sense RNA signal. Briefly, the protocol involves permeabilizing the exponentially growing yeast cells, allowing the transcripts that initiated in vivo to elongate in the presence of the BrUTP nucleotide, purifying BrUTP-labelled RNA by the affinity approach, reverse transcribing the purified nascent RNA and amplifying the cDNA using strand-specific primers flanking the promoter and the terminator regions of the gene2." @default.
- W4200295615 created "2021-12-31" @default.
- W4200295615 creator A5023763143 @default.
- W4200295615 creator A5034317620 @default.
- W4200295615 creator A5044716428 @default.
- W4200295615 creator A5048572637 @default.
- W4200295615 creator A5066463135 @default.
- W4200295615 creator A5089035479 @default.
- W4200295615 date "2017-03-12" @default.
- W4200295615 modified "2023-10-16" @default.
- W4200295615 title "Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach" @default.
- W4200295615 doi "https://doi.org/10.3791/55446-v" @default.
- W4200295615 hasPublicationYear "2017" @default.
- W4200295615 type Work @default.
- W4200295615 citedByCount "0" @default.
- W4200295615 crossrefType "journal-article" @default.
- W4200295615 hasAuthorship W4200295615A5023763143 @default.
- W4200295615 hasAuthorship W4200295615A5034317620 @default.
- W4200295615 hasAuthorship W4200295615A5044716428 @default.
- W4200295615 hasAuthorship W4200295615A5048572637 @default.
- W4200295615 hasAuthorship W4200295615A5066463135 @default.
- W4200295615 hasAuthorship W4200295615A5089035479 @default.
- W4200295615 hasBestOaLocation W42002956152 @default.
- W4200295615 hasConcept C101762097 @default.
- W4200295615 hasConcept C104317684 @default.
- W4200295615 hasConcept C116403925 @default.
- W4200295615 hasConcept C121332964 @default.
- W4200295615 hasConcept C1276947 @default.
- W4200295615 hasConcept C138885662 @default.
- W4200295615 hasConcept C144825837 @default.
- W4200295615 hasConcept C150194340 @default.
- W4200295615 hasConcept C153911025 @default.
- W4200295615 hasConcept C172768829 @default.
- W4200295615 hasConcept C178046730 @default.
- W4200295615 hasConcept C179926584 @default.
- W4200295615 hasConcept C2776449523 @default.
- W4200295615 hasConcept C41895202 @default.
- W4200295615 hasConcept C51807208 @default.
- W4200295615 hasConcept C54355233 @default.
- W4200295615 hasConcept C64350747 @default.
- W4200295615 hasConcept C67705224 @default.
- W4200295615 hasConcept C82381507 @default.
- W4200295615 hasConcept C86803240 @default.
- W4200295615 hasConceptScore W4200295615C101762097 @default.
- W4200295615 hasConceptScore W4200295615C104317684 @default.
- W4200295615 hasConceptScore W4200295615C116403925 @default.
- W4200295615 hasConceptScore W4200295615C121332964 @default.
- W4200295615 hasConceptScore W4200295615C1276947 @default.
- W4200295615 hasConceptScore W4200295615C138885662 @default.
- W4200295615 hasConceptScore W4200295615C144825837 @default.
- W4200295615 hasConceptScore W4200295615C150194340 @default.
- W4200295615 hasConceptScore W4200295615C153911025 @default.
- W4200295615 hasConceptScore W4200295615C172768829 @default.
- W4200295615 hasConceptScore W4200295615C178046730 @default.
- W4200295615 hasConceptScore W4200295615C179926584 @default.
- W4200295615 hasConceptScore W4200295615C2776449523 @default.
- W4200295615 hasConceptScore W4200295615C41895202 @default.
- W4200295615 hasConceptScore W4200295615C51807208 @default.
- W4200295615 hasConceptScore W4200295615C54355233 @default.
- W4200295615 hasConceptScore W4200295615C64350747 @default.
- W4200295615 hasConceptScore W4200295615C67705224 @default.
- W4200295615 hasConceptScore W4200295615C82381507 @default.
- W4200295615 hasConceptScore W4200295615C86803240 @default.
- W4200295615 hasIssue "121" @default.
- W4200295615 hasLocation W42002956151 @default.
- W4200295615 hasLocation W42002956152 @default.
- W4200295615 hasOpenAccess W4200295615 @default.
- W4200295615 hasPrimaryLocation W42002956151 @default.
- W4200295615 hasRelatedWork W1555221542 @default.
- W4200295615 hasRelatedWork W1799149248 @default.
- W4200295615 hasRelatedWork W2035601659 @default.
- W4200295615 hasRelatedWork W2037031854 @default.
- W4200295615 hasRelatedWork W2087667588 @default.
- W4200295615 hasRelatedWork W2101005129 @default.
- W4200295615 hasRelatedWork W2146975493 @default.
- W4200295615 hasRelatedWork W2921178186 @default.
- W4200295615 hasRelatedWork W3118427689 @default.
- W4200295615 hasRelatedWork W2397929048 @default.
- W4200295615 isParatext "false" @default.
- W4200295615 isRetracted "false" @default.
- W4200295615 workType "article" @default.