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- W4200301015 abstract "Compared to other tissues, white adipose tissue has a considerably less RNA and protein content for downstream applications such as real-time PCR and Western Blot, since it mostly contains lipids. RNA isolation from adipose tissue samples is also challenging as extra steps are required to avoid these lipids. Here, we present a procedure to collect three anatomically different white adipose tissues from mice, to process these samples and perform RNA isolation. We further describe the synthesis of cDNA and gene expression experiments using real-time PCR. The hereby described protocol allows the reduction of contamination from the animal's hair and blood on fat pads as well as cross-contamination between different fat pads during tissue collection. It has also been optimized to ensure adequate quantity and quality of the RNA extracted. This protocol can be widely applied to any mouse model where adipose tissue samples are required for routine experiments such as real-time PCR but is not intended for isolation from primary adipocytes cell culture." @default.
- W4200301015 created "2021-12-31" @default.
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- W4200301015 date "2018-01-31" @default.
- W4200301015 modified "2023-09-30" @default.
- W4200301015 title "Mouse Adipose Tissue Collection and Processing for RNA Analysis" @default.
- W4200301015 doi "https://doi.org/10.3791/57026-v" @default.
- W4200301015 hasPublicationYear "2018" @default.
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